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Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing

In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current mo...

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Autores principales: Park, Tae-Min, Kang, Donggu, Jang, Ilho, Yun, Won-Soo, Shim, Jin-Hyung, Jeong, Young Hun, Kwak, Jong-Young, Yoon, Sik, Jin, Songwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713317/
https://www.ncbi.nlm.nih.gov/pubmed/29112150
http://dx.doi.org/10.3390/ijms18112348
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author Park, Tae-Min
Kang, Donggu
Jang, Ilho
Yun, Won-Soo
Shim, Jin-Hyung
Jeong, Young Hun
Kwak, Jong-Young
Yoon, Sik
Jin, Songwan
author_facet Park, Tae-Min
Kang, Donggu
Jang, Ilho
Yun, Won-Soo
Shim, Jin-Hyung
Jeong, Young Hun
Kwak, Jong-Young
Yoon, Sik
Jin, Songwan
author_sort Park, Tae-Min
collection PubMed
description In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current models and humans. Here, we fabricated a cancer microtissue array in a multi-well format that exhibits heterogeneous and batch-to-batch structure by continuous deposition of collagen-suspended Hela cells on a fibroblast-layered nanofibrous membrane via inkjet printing. Expression of both Matrix Metalloproteinase 2 (MMP2) and Matrix Metalloproteinase 9 (MMP9) was higher in cancer microtissues than in fibroblast-free microtissues. The fabricated microtissues were treated with an anticancer drug, and high drug resistance to doxorubicin occurred in cancer microtissues but not in fibroblast-free microtissues. These results introduce an inkjet printing fabrication method for cancer microtissue arrays, which can be used for various applications such as early drug screening and gradual 3D cancer studies.
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spelling pubmed-57133172017-12-07 Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing Park, Tae-Min Kang, Donggu Jang, Ilho Yun, Won-Soo Shim, Jin-Hyung Jeong, Young Hun Kwak, Jong-Young Yoon, Sik Jin, Songwan Int J Mol Sci Article In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current models and humans. Here, we fabricated a cancer microtissue array in a multi-well format that exhibits heterogeneous and batch-to-batch structure by continuous deposition of collagen-suspended Hela cells on a fibroblast-layered nanofibrous membrane via inkjet printing. Expression of both Matrix Metalloproteinase 2 (MMP2) and Matrix Metalloproteinase 9 (MMP9) was higher in cancer microtissues than in fibroblast-free microtissues. The fabricated microtissues were treated with an anticancer drug, and high drug resistance to doxorubicin occurred in cancer microtissues but not in fibroblast-free microtissues. These results introduce an inkjet printing fabrication method for cancer microtissue arrays, which can be used for various applications such as early drug screening and gradual 3D cancer studies. MDPI 2017-11-07 /pmc/articles/PMC5713317/ /pubmed/29112150 http://dx.doi.org/10.3390/ijms18112348 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Tae-Min
Kang, Donggu
Jang, Ilho
Yun, Won-Soo
Shim, Jin-Hyung
Jeong, Young Hun
Kwak, Jong-Young
Yoon, Sik
Jin, Songwan
Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing
title Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing
title_full Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing
title_fullStr Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing
title_full_unstemmed Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing
title_short Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing
title_sort fabrication of in vitro cancer microtissue array on fibroblast-layered nanofibrous membrane by inkjet printing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713317/
https://www.ncbi.nlm.nih.gov/pubmed/29112150
http://dx.doi.org/10.3390/ijms18112348
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