Cargando…

The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles

Glioblastoma multiforme (GBM) is the most common form of malignant glioma. Recent studies point out that gliomas exploit ion channels and transporters, including Na, K-ATPase, to sustain their singular growth and invasion as they invade the brain parenchyma. Moreover, the different isoforms of the β...

Descripción completa

Detalles Bibliográficos
Autores principales: Rotoli, Deborah, Cejas, Mariana-Mayela, Maeso, María-del-Carmen, Pérez-Rodríguez, Natalia-Dolores, Morales, Manuel, Ávila, Julio, Mobasheri, Ali, Martín-Vasallo, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713338/
https://www.ncbi.nlm.nih.gov/pubmed/29117147
http://dx.doi.org/10.3390/ijms18112369
_version_ 1783283401662398464
author Rotoli, Deborah
Cejas, Mariana-Mayela
Maeso, María-del-Carmen
Pérez-Rodríguez, Natalia-Dolores
Morales, Manuel
Ávila, Julio
Mobasheri, Ali
Martín-Vasallo, Pablo
author_facet Rotoli, Deborah
Cejas, Mariana-Mayela
Maeso, María-del-Carmen
Pérez-Rodríguez, Natalia-Dolores
Morales, Manuel
Ávila, Julio
Mobasheri, Ali
Martín-Vasallo, Pablo
author_sort Rotoli, Deborah
collection PubMed
description Glioblastoma multiforme (GBM) is the most common form of malignant glioma. Recent studies point out that gliomas exploit ion channels and transporters, including Na, K-ATPase, to sustain their singular growth and invasion as they invade the brain parenchyma. Moreover, the different isoforms of the β-subunit of Na, K-ATPase have been implicated in regulating cellular dynamics, particularly during cancer progression. The aim of this study was to determine the Na, K-ATPase β subunit isoform subcellular expression patterns in all cell types responsible for microenvironment heterogeneity of GBM using immunohistochemical analysis. All three isoforms, β1, β2/AMOG (Adhesion Molecule On Glia) and β3, were found to be expressed in GBM samples. Generally, β1 isoform was not expressed by astrocytes, in both primary and secondary GBM, although other cell types (endothelial cells, pericytes, telocytes, macrophages) did express this isoform. β2/AMOG and β3 positive expression was observed in the cytoplasm, membrane and nuclear envelope of astrocytes and GFAP (Glial Fibrillary Acidic Protein) negative cells. Interestingly, differences in isoforms expression have been observed between primary and secondary GBM: in secondary GBM, β2 isoform expression in astrocytes was lower than that observed in primary GBM, while the expression of the β3 subunit was more intense. These changes in β subunit isoforms expression in GBM could be related to a different ionic handling, to a different relationship between astrocyte and neuron (β2/AMOG) and to changes in the moonlighting roles of Na, K-ATPase β subunits as adaptor proteins and transcription factors.
format Online
Article
Text
id pubmed-5713338
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57133382017-12-07 The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles Rotoli, Deborah Cejas, Mariana-Mayela Maeso, María-del-Carmen Pérez-Rodríguez, Natalia-Dolores Morales, Manuel Ávila, Julio Mobasheri, Ali Martín-Vasallo, Pablo Int J Mol Sci Article Glioblastoma multiforme (GBM) is the most common form of malignant glioma. Recent studies point out that gliomas exploit ion channels and transporters, including Na, K-ATPase, to sustain their singular growth and invasion as they invade the brain parenchyma. Moreover, the different isoforms of the β-subunit of Na, K-ATPase have been implicated in regulating cellular dynamics, particularly during cancer progression. The aim of this study was to determine the Na, K-ATPase β subunit isoform subcellular expression patterns in all cell types responsible for microenvironment heterogeneity of GBM using immunohistochemical analysis. All three isoforms, β1, β2/AMOG (Adhesion Molecule On Glia) and β3, were found to be expressed in GBM samples. Generally, β1 isoform was not expressed by astrocytes, in both primary and secondary GBM, although other cell types (endothelial cells, pericytes, telocytes, macrophages) did express this isoform. β2/AMOG and β3 positive expression was observed in the cytoplasm, membrane and nuclear envelope of astrocytes and GFAP (Glial Fibrillary Acidic Protein) negative cells. Interestingly, differences in isoforms expression have been observed between primary and secondary GBM: in secondary GBM, β2 isoform expression in astrocytes was lower than that observed in primary GBM, while the expression of the β3 subunit was more intense. These changes in β subunit isoforms expression in GBM could be related to a different ionic handling, to a different relationship between astrocyte and neuron (β2/AMOG) and to changes in the moonlighting roles of Na, K-ATPase β subunits as adaptor proteins and transcription factors. MDPI 2017-11-08 /pmc/articles/PMC5713338/ /pubmed/29117147 http://dx.doi.org/10.3390/ijms18112369 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rotoli, Deborah
Cejas, Mariana-Mayela
Maeso, María-del-Carmen
Pérez-Rodríguez, Natalia-Dolores
Morales, Manuel
Ávila, Julio
Mobasheri, Ali
Martín-Vasallo, Pablo
The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles
title The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles
title_full The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles
title_fullStr The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles
title_full_unstemmed The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles
title_short The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles
title_sort na, k-atpase β-subunit isoforms expression in glioblastoma multiforme: moonlighting roles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713338/
https://www.ncbi.nlm.nih.gov/pubmed/29117147
http://dx.doi.org/10.3390/ijms18112369
work_keys_str_mv AT rotolideborah thenakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT cejasmarianamayela thenakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT maesomariadelcarmen thenakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT perezrodrigueznataliadolores thenakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT moralesmanuel thenakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT avilajulio thenakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT mobasheriali thenakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT martinvasallopablo thenakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT rotolideborah nakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT cejasmarianamayela nakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT maesomariadelcarmen nakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT perezrodrigueznataliadolores nakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT moralesmanuel nakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT avilajulio nakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT mobasheriali nakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles
AT martinvasallopablo nakatpasebsubunitisoformsexpressioninglioblastomamultiformemoonlightingroles