Cargando…
Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), one of the wild relatives of wheat, exhibits resistance to stripe rust. In this study, wheat-A. crist...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713371/ https://www.ncbi.nlm.nih.gov/pubmed/29137188 http://dx.doi.org/10.3390/ijms18112403 |
_version_ | 1783283409549787136 |
---|---|
author | Zhang, Zhi Song, Liqiang Han, Haiming Zhou, Shenghui Zhang, Jinpeng Yang, Xinming Li, Xiuquan Liu, Weihua Li, Lihui |
author_facet | Zhang, Zhi Song, Liqiang Han, Haiming Zhou, Shenghui Zhang, Jinpeng Yang, Xinming Li, Xiuquan Liu, Weihua Li, Lihui |
author_sort | Zhang, Zhi |
collection | PubMed |
description | Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), one of the wild relatives of wheat, exhibits resistance to stripe rust. In this study, wheat-A. cristatum 6P disomic addition line 4844-12 also exhibited resistance to stripe rust. To identify the stripe rust resistance locus from A. cristatum 6P, ten translocation lines, five deletion lines and the BC(2)F(2) and BC(3)F(2) populations of two wheat-A. cristatum 6P whole-arm translocation lines were tested with a mixture of two races of Pst in two sites during 2015–2016 and 2016–2017, being genotyped with genomic in situ hybridization (GISH) and molecular markers. The result indicated that the locus conferring stripe rust resistance was located on the terminal 20% of 6P short arm’s length. Twenty-nine 6P-specific sequence-tagged-site (STS) markers mapped on the resistance locus have been acquired, which will be helpful for the fine mapping of the stripe rust resistance locus. The stripe rust-resistant translocation lines were found to carry some favorable agronomic traits, which could facilitate their use in wheat improvement. Collectively, the stripe rust resistance locus from A. cristatum 6P could be a novel resistance source and the screened stripe rust-resistant materials will be valuable for wheat disease breeding. |
format | Online Article Text |
id | pubmed-5713371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-57133712017-12-07 Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat Zhang, Zhi Song, Liqiang Han, Haiming Zhou, Shenghui Zhang, Jinpeng Yang, Xinming Li, Xiuquan Liu, Weihua Li, Lihui Int J Mol Sci Article Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), one of the wild relatives of wheat, exhibits resistance to stripe rust. In this study, wheat-A. cristatum 6P disomic addition line 4844-12 also exhibited resistance to stripe rust. To identify the stripe rust resistance locus from A. cristatum 6P, ten translocation lines, five deletion lines and the BC(2)F(2) and BC(3)F(2) populations of two wheat-A. cristatum 6P whole-arm translocation lines were tested with a mixture of two races of Pst in two sites during 2015–2016 and 2016–2017, being genotyped with genomic in situ hybridization (GISH) and molecular markers. The result indicated that the locus conferring stripe rust resistance was located on the terminal 20% of 6P short arm’s length. Twenty-nine 6P-specific sequence-tagged-site (STS) markers mapped on the resistance locus have been acquired, which will be helpful for the fine mapping of the stripe rust resistance locus. The stripe rust-resistant translocation lines were found to carry some favorable agronomic traits, which could facilitate their use in wheat improvement. Collectively, the stripe rust resistance locus from A. cristatum 6P could be a novel resistance source and the screened stripe rust-resistant materials will be valuable for wheat disease breeding. MDPI 2017-11-13 /pmc/articles/PMC5713371/ /pubmed/29137188 http://dx.doi.org/10.3390/ijms18112403 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Zhi Song, Liqiang Han, Haiming Zhou, Shenghui Zhang, Jinpeng Yang, Xinming Li, Xiuquan Liu, Weihua Li, Lihui Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat |
title | Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat |
title_full | Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat |
title_fullStr | Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat |
title_full_unstemmed | Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat |
title_short | Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat |
title_sort | physical localization of a locus from agropyron cristatum conferring resistance to stripe rust in common wheat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713371/ https://www.ncbi.nlm.nih.gov/pubmed/29137188 http://dx.doi.org/10.3390/ijms18112403 |
work_keys_str_mv | AT zhangzhi physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat AT songliqiang physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat AT hanhaiming physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat AT zhoushenghui physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat AT zhangjinpeng physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat AT yangxinming physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat AT lixiuquan physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat AT liuweihua physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat AT lilihui physicallocalizationofalocusfromagropyroncristatumconferringresistancetostriperustincommonwheat |