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Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd

Oxidosqualene cyclases (OSCs) are enzymes that play a key role in control of the biosynthesis of phytosterols and triterpene saponins. In order to uncover OSC genes from Polygala tenuifolia seedlings induced by methyl jasmonate (MeJA), RNA-sequencing analysis was performed using the Illumina sequenc...

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Autores principales: Jin, Mei Lan, Lee, Woo Moon, Kim, Ok Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713394/
https://www.ncbi.nlm.nih.gov/pubmed/29140303
http://dx.doi.org/10.3390/ijms18112426
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author Jin, Mei Lan
Lee, Woo Moon
Kim, Ok Tae
author_facet Jin, Mei Lan
Lee, Woo Moon
Kim, Ok Tae
author_sort Jin, Mei Lan
collection PubMed
description Oxidosqualene cyclases (OSCs) are enzymes that play a key role in control of the biosynthesis of phytosterols and triterpene saponins. In order to uncover OSC genes from Polygala tenuifolia seedlings induced by methyl jasmonate (MeJA), RNA-sequencing analysis was performed using the Illumina sequencing platform. A total of 148,488,632 high-quality reads from two samples (control and the MeJA treated) were generated. We screened genes related to phytosterol and triterpene saponin biosynthesis and analyzed the transcriptional changes of differentially expressed unigene (DEUG) values calculated by fragments per kilobase million (FPKM). In our datasets, two full-length cDNAs of putative OSC genes, PtCAS1, and PtCAS2, were found, in addition to the PtBS (β-amyrin synthase) gene reported in our previous studies and the two cycloartenol synthase genes of P. tenuifolia. All genes were isolated and characterized in yeast cells. The functional expression of the two PtCAS genes in yeast cells showed that the genes all produce a cycloartenol as the sole product. When qRT-PCR analysis from different tissues was performed, the expressions of PtCAS1 and PtCAS2 were highest in flowers and roots, respectively. After MeJA treatment, the transcripts of PtCAS1 and PtCAS2 genes increased by 1.5- and 2-fold, respectively. Given these results, we discuss the potential roles of the two PtCAS genes in relation to triterpenoid biosynthesis.
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spelling pubmed-57133942017-12-07 Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd Jin, Mei Lan Lee, Woo Moon Kim, Ok Tae Int J Mol Sci Article Oxidosqualene cyclases (OSCs) are enzymes that play a key role in control of the biosynthesis of phytosterols and triterpene saponins. In order to uncover OSC genes from Polygala tenuifolia seedlings induced by methyl jasmonate (MeJA), RNA-sequencing analysis was performed using the Illumina sequencing platform. A total of 148,488,632 high-quality reads from two samples (control and the MeJA treated) were generated. We screened genes related to phytosterol and triterpene saponin biosynthesis and analyzed the transcriptional changes of differentially expressed unigene (DEUG) values calculated by fragments per kilobase million (FPKM). In our datasets, two full-length cDNAs of putative OSC genes, PtCAS1, and PtCAS2, were found, in addition to the PtBS (β-amyrin synthase) gene reported in our previous studies and the two cycloartenol synthase genes of P. tenuifolia. All genes were isolated and characterized in yeast cells. The functional expression of the two PtCAS genes in yeast cells showed that the genes all produce a cycloartenol as the sole product. When qRT-PCR analysis from different tissues was performed, the expressions of PtCAS1 and PtCAS2 were highest in flowers and roots, respectively. After MeJA treatment, the transcripts of PtCAS1 and PtCAS2 genes increased by 1.5- and 2-fold, respectively. Given these results, we discuss the potential roles of the two PtCAS genes in relation to triterpenoid biosynthesis. MDPI 2017-11-15 /pmc/articles/PMC5713394/ /pubmed/29140303 http://dx.doi.org/10.3390/ijms18112426 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jin, Mei Lan
Lee, Woo Moon
Kim, Ok Tae
Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd
title Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd
title_full Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd
title_fullStr Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd
title_full_unstemmed Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd
title_short Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd
title_sort two cycloartenol synthases for phytosterol biosynthesis in polygala tenuifolia willd
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713394/
https://www.ncbi.nlm.nih.gov/pubmed/29140303
http://dx.doi.org/10.3390/ijms18112426
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