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Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles

Although nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated in several cell lines with different methods and on multiple nanoparticle batche...

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Autores principales: Sulheim, Einar, Iversen, Tore-Geir, To Nakstad, Vu, Klinkenberg, Geir, Sletta, Håvard, Schmid, Ruth, Hatletveit, Anne Rein, Wågbø, Ane Marit, Sundan, Anders, Skotland, Tore, Sandvig, Kirsten, Mørch, Ýrr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713421/
https://www.ncbi.nlm.nih.gov/pubmed/29156588
http://dx.doi.org/10.3390/ijms18112454
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author Sulheim, Einar
Iversen, Tore-Geir
To Nakstad, Vu
Klinkenberg, Geir
Sletta, Håvard
Schmid, Ruth
Hatletveit, Anne Rein
Wågbø, Ane Marit
Sundan, Anders
Skotland, Tore
Sandvig, Kirsten
Mørch, Ýrr
author_facet Sulheim, Einar
Iversen, Tore-Geir
To Nakstad, Vu
Klinkenberg, Geir
Sletta, Håvard
Schmid, Ruth
Hatletveit, Anne Rein
Wågbø, Ane Marit
Sundan, Anders
Skotland, Tore
Sandvig, Kirsten
Mørch, Ýrr
author_sort Sulheim, Einar
collection PubMed
description Although nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated in several cell lines with different methods and on multiple nanoparticle batches. Here we report the toxicity of poly(alkyl cyanoacrylate) nanoparticles in 12 different cell lines after synthesizing and analyzing 19 different nanoparticle batches and report that large variations were obtained when using different cell lines or various toxicity assays. Surprisingly, we found that nanoparticles with intermediate degradation rates were less toxic than particles that were degraded faster or more slowly in a cell-free system. The toxicity did not vary significantly with either the three different combinations of polyethylene glycol surfactants or with particle size (range 100–200 nm). No acute pro- or anti-inflammatory activity on cells in whole blood was observed.
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spelling pubmed-57134212017-12-07 Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles Sulheim, Einar Iversen, Tore-Geir To Nakstad, Vu Klinkenberg, Geir Sletta, Håvard Schmid, Ruth Hatletveit, Anne Rein Wågbø, Ane Marit Sundan, Anders Skotland, Tore Sandvig, Kirsten Mørch, Ýrr Int J Mol Sci Article Although nanotoxicology has become a large research field, assessment of cytotoxicity is often reduced to analysis of one cell line only. Cytotoxicity of nanoparticles is complex and should, preferentially, be evaluated in several cell lines with different methods and on multiple nanoparticle batches. Here we report the toxicity of poly(alkyl cyanoacrylate) nanoparticles in 12 different cell lines after synthesizing and analyzing 19 different nanoparticle batches and report that large variations were obtained when using different cell lines or various toxicity assays. Surprisingly, we found that nanoparticles with intermediate degradation rates were less toxic than particles that were degraded faster or more slowly in a cell-free system. The toxicity did not vary significantly with either the three different combinations of polyethylene glycol surfactants or with particle size (range 100–200 nm). No acute pro- or anti-inflammatory activity on cells in whole blood was observed. MDPI 2017-11-18 /pmc/articles/PMC5713421/ /pubmed/29156588 http://dx.doi.org/10.3390/ijms18112454 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sulheim, Einar
Iversen, Tore-Geir
To Nakstad, Vu
Klinkenberg, Geir
Sletta, Håvard
Schmid, Ruth
Hatletveit, Anne Rein
Wågbø, Ane Marit
Sundan, Anders
Skotland, Tore
Sandvig, Kirsten
Mørch, Ýrr
Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles
title Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles
title_full Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles
title_fullStr Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles
title_full_unstemmed Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles
title_short Cytotoxicity of Poly(Alkyl Cyanoacrylate) Nanoparticles
title_sort cytotoxicity of poly(alkyl cyanoacrylate) nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5713421/
https://www.ncbi.nlm.nih.gov/pubmed/29156588
http://dx.doi.org/10.3390/ijms18112454
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