CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level

CRISPR/Cas9 is a powerful gene editing tool for gene knockout studies and functional genomic screens. Successful implementation of CRISPR often requires Cas9 to elicit efficient target knockout in a population of cells. In this study, we investigated the role of several key factors, including variat...

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Detalles Bibliográficos
Autores principales: Yuen, Garmen, Khan, Fehad J., Gao, Shaojian, Stommel, Jayne M., Batchelor, Eric, Wu, Xiaolin, Luo, Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5714203/
https://www.ncbi.nlm.nih.gov/pubmed/29036671
http://dx.doi.org/10.1093/nar/gkx843

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