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Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells

The cutaneous wound-healing program is a product of a complex interplay among diverse cell types within the skin. One fundamental process that is mediated by these reciprocal interactions is the mobilization of local stem cell pools to promote tissue regeneration and repair. Using the ablation of ep...

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Autores principales: Lee, Pedro, Gund, Rupali, Dutta, Abhik, Pincha, Neha, Rana, Isha, Ghosh, Subhasri, Witherden, Deborah, Kandyba, Eve, MacLeod, Amanda, Kobielak, Krzysztof, Havran, Wendy L, Jamora, Colin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5714500/
https://www.ncbi.nlm.nih.gov/pubmed/29199946
http://dx.doi.org/10.7554/eLife.28875
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author Lee, Pedro
Gund, Rupali
Dutta, Abhik
Pincha, Neha
Rana, Isha
Ghosh, Subhasri
Witherden, Deborah
Kandyba, Eve
MacLeod, Amanda
Kobielak, Krzysztof
Havran, Wendy L
Jamora, Colin
author_facet Lee, Pedro
Gund, Rupali
Dutta, Abhik
Pincha, Neha
Rana, Isha
Ghosh, Subhasri
Witherden, Deborah
Kandyba, Eve
MacLeod, Amanda
Kobielak, Krzysztof
Havran, Wendy L
Jamora, Colin
author_sort Lee, Pedro
collection PubMed
description The cutaneous wound-healing program is a product of a complex interplay among diverse cell types within the skin. One fundamental process that is mediated by these reciprocal interactions is the mobilization of local stem cell pools to promote tissue regeneration and repair. Using the ablation of epidermal caspase-8 as a model of wound healing in Mus musculus, we analyzed the signaling components responsible for epithelial stem cell proliferation. We found that IL-1α and IL-7 secreted from keratinocytes work in tandem to expand the activated population of resident epidermal γδT-cells. A downstream effect of activated γδT-cells is the preferential proliferation of hair follicle stem cells. By contrast, IL-1α-dependent stimulation of dermal fibroblasts optimally stimulates epidermal stem cell proliferation. These findings provide new mechanistic insights into the regulation and function of epidermal cell–immune cell interactions and into how components that are classically associated with inflammation can differentially influence distinct stem cell niches within a tissue.
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spelling pubmed-57145002017-12-06 Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells Lee, Pedro Gund, Rupali Dutta, Abhik Pincha, Neha Rana, Isha Ghosh, Subhasri Witherden, Deborah Kandyba, Eve MacLeod, Amanda Kobielak, Krzysztof Havran, Wendy L Jamora, Colin eLife Cell Biology The cutaneous wound-healing program is a product of a complex interplay among diverse cell types within the skin. One fundamental process that is mediated by these reciprocal interactions is the mobilization of local stem cell pools to promote tissue regeneration and repair. Using the ablation of epidermal caspase-8 as a model of wound healing in Mus musculus, we analyzed the signaling components responsible for epithelial stem cell proliferation. We found that IL-1α and IL-7 secreted from keratinocytes work in tandem to expand the activated population of resident epidermal γδT-cells. A downstream effect of activated γδT-cells is the preferential proliferation of hair follicle stem cells. By contrast, IL-1α-dependent stimulation of dermal fibroblasts optimally stimulates epidermal stem cell proliferation. These findings provide new mechanistic insights into the regulation and function of epidermal cell–immune cell interactions and into how components that are classically associated with inflammation can differentially influence distinct stem cell niches within a tissue. eLife Sciences Publications, Ltd 2017-12-04 /pmc/articles/PMC5714500/ /pubmed/29199946 http://dx.doi.org/10.7554/eLife.28875 Text en © 2017, Lee et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Lee, Pedro
Gund, Rupali
Dutta, Abhik
Pincha, Neha
Rana, Isha
Ghosh, Subhasri
Witherden, Deborah
Kandyba, Eve
MacLeod, Amanda
Kobielak, Krzysztof
Havran, Wendy L
Jamora, Colin
Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells
title Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells
title_full Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells
title_fullStr Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells
title_full_unstemmed Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells
title_short Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells
title_sort stimulation of hair follicle stem cell proliferation through an il-1 dependent activation of γδt-cells
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5714500/
https://www.ncbi.nlm.nih.gov/pubmed/29199946
http://dx.doi.org/10.7554/eLife.28875
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