Cargando…

Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains

RNA-guided endonucleases (RGENs) have invigorated the field of site-specific nucleases. The success of Streptococcus pyogenes Cas9 (SpCas9) has led to the discovery of several other CRISPR-associated RGENs. As more RGENs become available, it will be necessary to refine their activity before they can...

Descripción completa

Detalles Bibliográficos
Autores principales:	Spencer, Jeffrey M., Zhang, Xiaoliu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715146/ https://www.ncbi.nlm.nih.gov/pubmed/29203891 http://dx.doi.org/10.1038/s41598-017-17081-y

Ejemplares similares

Structural and functional insights into the bona fide catalytic state of Streptococcus pyogenes Cas9 HNH nuclease domain
por: Zuo, Zhicheng, et al.
Publicado: (2019)

CRISPR/Cas9 recombineering‐mediated deep mutational scanning of essential genes in Escherichia coli
por: Choudhury, Alaksh, et al.
Publicado: (2020)

Molecular mechanisms of Streptococcus pyogenes Cas9: a single-molecule perspective
por: Zhang, Qian, et al.
Publicado: (2021)

CRISPR-Cas in Streptococcus pyogenes
por: Le Rhun, Anaïs, et al.
Publicado: (2019)

Active-Site Models of Streptococcus pyogenes Cas9 in DNA Cleavage State
por: Tang, Honghai, et al.
Publicado: (2021)

Functional and Proteomic Analysis of Streptococcus pyogenes Virulence Upon Loss of Its Native Cas9 Nuclease
por: Gao, Nina J., et al.
Publicado: (2019)

Mapping the functional landscape of the receptor binding domain of T7 bacteriophage by deep mutational scanning
por: Huss, Phil, et al.
Publicado: (2021)

Erratum: Active-Site Models of Streptococcus pyogenes Cas9 in DNA Cleavage State
por: Office, Frontiers Production
Publicado: (2021)

Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys
por: Luo, Xin, et al.
Publicado: (2019)

Conserved regulatory motifs in the juxtamembrane domain and kinase N-lobe revealed through deep mutational scanning of the MET receptor tyrosine kinase domain
por: Estevam, Gabriella O., et al.
Publicado: (2023)

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9
por: Collias, D., et al.
Publicado: (2020)

Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity
por: Aschenbrenner, Sabine, et al.
Publicado: (2020)

Deep mutational scanning of an RRM domain of the Saccharomyces cerevisiae poly(A)-binding protein
por: Melamed, Daniel, et al.
Publicado: (2013)

Probing biophysical sequence constraints within the transmembrane domains of rhodopsin by deep mutational scanning
por: Penn, Wesley D., et al.
Publicado: (2020)

Deep Mutational Scanning Reveals the Structural Basis for α-Synuclein Activity
por: Newberry, Robert W., et al.
Publicado: (2020)

Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding
por: Starr, Tyler N., et al.
Publicado: (2020)

Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding
por: Starr, Tyler N., et al.
Publicado: (2020)

Molecular and functional correction of a deep intronic splicing mutation in CFTR by CRISPR-Cas9 gene editing
por: Walker, Amy J., et al.
Publicado: (2023)

Novel human mutation and CRISPR/Cas genome-edited mice reveal the importance of C-terminal domain of MSX1 in tooth and palate development
por: Mitsui, Silvia Naomi, et al.
Publicado: (2016)

Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9
por: Zhu, Xing, et al.
Publicado: (2019)

Structural and functional characterization of G protein–coupled receptors with deep mutational scanning
por: Jones, Eric M, et al.
Publicado: (2020)

Applications of Deep Mutational Scanning in Virology
por: Burton, Thomas D., et al.
Publicado: (2021)

CYP2C9 and CYP2C19: Deep Mutational Scanning and Functional Characterization of Genomic Missense Variants
por: Zhang, Lingxin, et al.
Publicado: (2020)

SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with high generalization performance
por: Kim, Hui Kwon, et al.
Publicado: (2019)

Deep mutational scanning reveals the functional constraints and evolutionary potential of the influenza A virus PB1 protein
por: Li, Yuan, et al.
Publicado: (2023)

Comprehensive Deep Mutational Scanning Reveals the Immune-Escaping Hotspots of SARS-CoV-2 Receptor-Binding Domain Targeting Neutralizing Antibodies
por: Tsai, Keng-Chang, et al.
Publicado: (2021)

Deep mutational scanning of the plasminogen activator inhibitor-1 functional landscape
por: Huttinger, Zachary M., et al.
Publicado: (2021)

In vivo multiplex gene targeting with Streptococcus pyogens and Campylobacter jejuni Cas9 for pancreatic cancer modeling in wild-type animal
por: Chang, Yoo Jin, et al.
Publicado: (2020)

Microfluidic deep mutational scanning of the human executioner caspases reveals differences in structure and regulation
por: Roychowdhury, Hridindu, et al.
Publicado: (2022)

Deep mutational scanning reveals the molecular determinants of RNA polymerase-mediated adaptation and tradeoffs
por: Choudhury, Alaksh, et al.
Publicado: (2023)

Structure and Dynamics of Cas9 HNH Domain Catalytic State
por: Zuo, Zhicheng, et al.
Publicado: (2017)

Deep mutational scanning of proteins in mammalian cells
por: Maes, Stefanie, et al.
Publicado: (2023)

Whole-genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine
por: Wang, Xianhang, et al.
Publicado: (2021)

Stabilization of the SARS-CoV-2 Receptor Binding Domain by Protein Core Redesign and Deep Mutational Scanning
por: Leonard, Alison C., et al.
Publicado: (2021)

Probing the Dynamics of Streptococcus pyogenes Cas9 Endonuclease Bound to the sgRNA Complex Using Hydrogen-Deuterium Exchange Mass Spectrometry
por: Zhdanova, Polina V., et al.
Publicado: (2022)

A conserved maternal-specific repressive domain in Zelda revealed by Cas9-mediated mutagenesis in Drosophila melanogaster
por: Hamm, Danielle C., et al.
Publicado: (2017)

Correction to: Microfluidic deep mutational scanning of the human executioner caspases reveals differences in structure and regulation
por: Roychowdhury, Hridindu, et al.
Publicado: (2022)

Precise CRISPR-Cas9-mediated mutation of a membrane trafficking domain in the Drosophila vesicular monoamine transporter gene
por: Asuncion, James D., et al.
Publicado: (2023)

Real‐time observation of flexible domain movements in CRISPR–Cas9
por: Osuka, Saki, et al.
Publicado: (2018)

A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the Yeast S. cerevisiae
por: Zhang, Yadan, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_75ghud4dc9od0g56bdrdnua0qm