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Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer’s disease diagnostics
Gene expression profiles reflect the biologically diverse activities of cells under specific cell environments. Using the transcriptional response of cultured cells to blood composition, we developed a litmus gene assay to discriminate blood samples reflecting different sample qualities or disease c...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715155/ https://www.ncbi.nlm.nih.gov/pubmed/29203810 http://dx.doi.org/10.1038/s41598-017-17293-2 |
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author | Shim, Sung-Mi Kim, Jong-Hoon Jeon, Jae-Pil |
author_facet | Shim, Sung-Mi Kim, Jong-Hoon Jeon, Jae-Pil |
author_sort | Shim, Sung-Mi |
collection | PubMed |
description | Gene expression profiles reflect the biologically diverse activities of cells under specific cell environments. Using the transcriptional response of cultured cells to blood composition, we developed a litmus gene assay to discriminate blood samples reflecting different sample qualities or disease conditions. This cell-based litmus gene assay identified six genes (CCL20, CEMIP, IL1B, IL8, PRG2, PTGS2) as potential biomarkers of plasma quality control and the SPC25 gene as a diagnostic biomarker of Alzheimer’s disease (AD). In addition, the SPC25 gene expression level was significantly increased in the cell-based assay using serum samples from patients with mild cognitive impairment (MCI). In conclusion, we demonstrated the effectiveness and potential of a litmus gene assay to detect the orchestrated effects of circulating systemic factors, leading to the successful diagnosis of AD and MCI. This method is broadly applicable to the diagnosis of disease subtypes or patho-physiological stages of complex diseases and tumors. |
format | Online Article Text |
id | pubmed-5715155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57151552017-12-08 Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer’s disease diagnostics Shim, Sung-Mi Kim, Jong-Hoon Jeon, Jae-Pil Sci Rep Article Gene expression profiles reflect the biologically diverse activities of cells under specific cell environments. Using the transcriptional response of cultured cells to blood composition, we developed a litmus gene assay to discriminate blood samples reflecting different sample qualities or disease conditions. This cell-based litmus gene assay identified six genes (CCL20, CEMIP, IL1B, IL8, PRG2, PTGS2) as potential biomarkers of plasma quality control and the SPC25 gene as a diagnostic biomarker of Alzheimer’s disease (AD). In addition, the SPC25 gene expression level was significantly increased in the cell-based assay using serum samples from patients with mild cognitive impairment (MCI). In conclusion, we demonstrated the effectiveness and potential of a litmus gene assay to detect the orchestrated effects of circulating systemic factors, leading to the successful diagnosis of AD and MCI. This method is broadly applicable to the diagnosis of disease subtypes or patho-physiological stages of complex diseases and tumors. Nature Publishing Group UK 2017-12-04 /pmc/articles/PMC5715155/ /pubmed/29203810 http://dx.doi.org/10.1038/s41598-017-17293-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shim, Sung-Mi Kim, Jong-Hoon Jeon, Jae-Pil Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer’s disease diagnostics |
title | Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer’s disease diagnostics |
title_full | Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer’s disease diagnostics |
title_fullStr | Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer’s disease diagnostics |
title_full_unstemmed | Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer’s disease diagnostics |
title_short | Effective litmus gene test for monitoring the quality of blood samples: Application to Alzheimer’s disease diagnostics |
title_sort | effective litmus gene test for monitoring the quality of blood samples: application to alzheimer’s disease diagnostics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715155/ https://www.ncbi.nlm.nih.gov/pubmed/29203810 http://dx.doi.org/10.1038/s41598-017-17293-2 |
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