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FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1
Flavin adenine dinucleotide (FAD), synthesized from riboflavin, is redox cofactor in energy production and plays an important role in cell survival. More recently, riboflavin deficiency has been linked to developmental disorders, but its role in stem cell differentiation remains unclear. Here, we sh...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715241/ https://www.ncbi.nlm.nih.gov/pubmed/29226080 http://dx.doi.org/10.1002/2211-5463.12331 |
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author | Hirano, Kazumi Namihira, Masakazu |
author_facet | Hirano, Kazumi Namihira, Masakazu |
author_sort | Hirano, Kazumi |
collection | PubMed |
description | Flavin adenine dinucleotide (FAD), synthesized from riboflavin, is redox cofactor in energy production and plays an important role in cell survival. More recently, riboflavin deficiency has been linked to developmental disorders, but its role in stem cell differentiation remains unclear. Here, we show that FAD treatment, using DMSO as a solvent, enabled an increase in the amount of intracellular FAD and promoted neuronal differentiation of human neural stem cells (NSCs) derived not only from fetal brain, but also from induced pluripotent stem cells. Depression of FAD‐dependent histone demethylase, lysine‐specific demethylase‐1 (LSD1), prevented FAD‐induced neuronal differentiation. Furthermore, FAD influx facilitated nuclear localization of LSD1 and its enzymatic activity. Together, these findings led us to propose that FAD contributes to proper neuronal production from NSCs in the human fetal brain during development. |
format | Online Article Text |
id | pubmed-5715241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57152412017-12-08 FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1 Hirano, Kazumi Namihira, Masakazu FEBS Open Bio Research Articles Flavin adenine dinucleotide (FAD), synthesized from riboflavin, is redox cofactor in energy production and plays an important role in cell survival. More recently, riboflavin deficiency has been linked to developmental disorders, but its role in stem cell differentiation remains unclear. Here, we show that FAD treatment, using DMSO as a solvent, enabled an increase in the amount of intracellular FAD and promoted neuronal differentiation of human neural stem cells (NSCs) derived not only from fetal brain, but also from induced pluripotent stem cells. Depression of FAD‐dependent histone demethylase, lysine‐specific demethylase‐1 (LSD1), prevented FAD‐induced neuronal differentiation. Furthermore, FAD influx facilitated nuclear localization of LSD1 and its enzymatic activity. Together, these findings led us to propose that FAD contributes to proper neuronal production from NSCs in the human fetal brain during development. John Wiley and Sons Inc. 2017-10-17 /pmc/articles/PMC5715241/ /pubmed/29226080 http://dx.doi.org/10.1002/2211-5463.12331 Text en © 2017 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Hirano, Kazumi Namihira, Masakazu FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1 |
title |
FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1 |
title_full |
FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1 |
title_fullStr |
FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1 |
title_full_unstemmed |
FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1 |
title_short |
FAD influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of LSD1 |
title_sort | fad influx enhances neuronal differentiation of human neural stem cells by facilitating nuclear localization of lsd1 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715241/ https://www.ncbi.nlm.nih.gov/pubmed/29226080 http://dx.doi.org/10.1002/2211-5463.12331 |
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