Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia

BACKGROUND AND OBJECTIVES: The cytotoxin-associated gene (cag) pathogenicity island is reported to be a major virulence factor of Helicobacter pylori infection. It is previously reported that the cagA-positive strains are more virulent, so it can be postulated that the cagA-positive gastritis will b...

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Autores principales: Fakhre-Yaseri, Hashem, Baradaran-Moghaddam, Ali, Shekaraby, Mehdi, Baradaran, Hamid Reza, Soltani-Arabshahi, Seyed Kamran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715283/
https://www.ncbi.nlm.nih.gov/pubmed/29214001
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author Fakhre-Yaseri, Hashem
Baradaran-Moghaddam, Ali
Shekaraby, Mehdi
Baradaran, Hamid Reza
Soltani-Arabshahi, Seyed Kamran
author_facet Fakhre-Yaseri, Hashem
Baradaran-Moghaddam, Ali
Shekaraby, Mehdi
Baradaran, Hamid Reza
Soltani-Arabshahi, Seyed Kamran
author_sort Fakhre-Yaseri, Hashem
collection PubMed
description BACKGROUND AND OBJECTIVES: The cytotoxin-associated gene (cag) pathogenicity island is reported to be a major virulence factor of Helicobacter pylori infection. It is previously reported that the cagA-positive strains are more virulent, so it can be postulated that the cagA-positive gastritis will be more severe and the serum immunoglobulin G (IgG) and A(IgA) anti-CagA antibody titer will be higher. The aim of this study was to compare the relationship between IgG and IgA anti-CagA antibody and the cagA gene expression in patients with dyspepsia. Serum samples obtained from 130 dyspeptic patients with positive H. pylori in histological and Geimsa staining were tested for serum IgG and IgA anti-CagA antibody using the enzyme-linked immunosorbent Assay. The expression of the cagA gene was determined using PCR on the biopsy samples, taken via endoscopy. RESULTS: In our material, the sensitivity of IgG anti-CagA antibody in identifying patients with a proven infection with the cagA-positive strains was 97.67%, and the negative likelihood ratios was 0.06. There was not significant correlation between serum IgA anti-CagA and the expression of the cagA gene among the dyspeptic patients. CONCLUSION: The IgG antibody titer was significantly higher in our patients with the cagA-positive H. pylori strain. However, in daily practice, the level of the IgG antibody titer cannot predict whether or not an individual carries a cagA-positive H. pylori strain, because there is a major overlap in the IgG antibody titer between the cagA-positive and cagA-negative patients.
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spelling pubmed-57152832017-12-06 Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia Fakhre-Yaseri, Hashem Baradaran-Moghaddam, Ali Shekaraby, Mehdi Baradaran, Hamid Reza Soltani-Arabshahi, Seyed Kamran Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: The cytotoxin-associated gene (cag) pathogenicity island is reported to be a major virulence factor of Helicobacter pylori infection. It is previously reported that the cagA-positive strains are more virulent, so it can be postulated that the cagA-positive gastritis will be more severe and the serum immunoglobulin G (IgG) and A(IgA) anti-CagA antibody titer will be higher. The aim of this study was to compare the relationship between IgG and IgA anti-CagA antibody and the cagA gene expression in patients with dyspepsia. Serum samples obtained from 130 dyspeptic patients with positive H. pylori in histological and Geimsa staining were tested for serum IgG and IgA anti-CagA antibody using the enzyme-linked immunosorbent Assay. The expression of the cagA gene was determined using PCR on the biopsy samples, taken via endoscopy. RESULTS: In our material, the sensitivity of IgG anti-CagA antibody in identifying patients with a proven infection with the cagA-positive strains was 97.67%, and the negative likelihood ratios was 0.06. There was not significant correlation between serum IgA anti-CagA and the expression of the cagA gene among the dyspeptic patients. CONCLUSION: The IgG antibody titer was significantly higher in our patients with the cagA-positive H. pylori strain. However, in daily practice, the level of the IgG antibody titer cannot predict whether or not an individual carries a cagA-positive H. pylori strain, because there is a major overlap in the IgG antibody titer between the cagA-positive and cagA-negative patients. Tehran University of Medical Sciences 2017-04 /pmc/articles/PMC5715283/ /pubmed/29214001 Text en Copyright© 2017 Iranian Neuroscience Society http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Fakhre-Yaseri, Hashem
Baradaran-Moghaddam, Ali
Shekaraby, Mehdi
Baradaran, Hamid Reza
Soltani-Arabshahi, Seyed Kamran
Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia
title Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia
title_full Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia
title_fullStr Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia
title_full_unstemmed Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia
title_short Evaluating the relationship between serum immunoglobulin G (IgG) and A (IgA) anti-CagA antibody and the cagA gene in patients with dyspepsia
title_sort evaluating the relationship between serum immunoglobulin g (igg) and a (iga) anti-caga antibody and the caga gene in patients with dyspepsia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715283/
https://www.ncbi.nlm.nih.gov/pubmed/29214001
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