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Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements

Plant phenomics offers unique opportunities to accelerate our understanding of gene function and plant response to different environments, and may be particularly useful for studying previously uncharacterized genes. One important type of poorly characterized genes is those derived from transposable...

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Autores principales: Joly-Lopez, Zoé, Forczek, Ewa, Vello, Emilio, Hoen, Douglas R., Tomita, Akiko, Bureau, Thomas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715367/
https://www.ncbi.nlm.nih.gov/pubmed/29250089
http://dx.doi.org/10.3389/fpls.2017.02027
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author Joly-Lopez, Zoé
Forczek, Ewa
Vello, Emilio
Hoen, Douglas R.
Tomita, Akiko
Bureau, Thomas E.
author_facet Joly-Lopez, Zoé
Forczek, Ewa
Vello, Emilio
Hoen, Douglas R.
Tomita, Akiko
Bureau, Thomas E.
author_sort Joly-Lopez, Zoé
collection PubMed
description Plant phenomics offers unique opportunities to accelerate our understanding of gene function and plant response to different environments, and may be particularly useful for studying previously uncharacterized genes. One important type of poorly characterized genes is those derived from transposable elements (TEs), which have departed from a mobility-driven lifestyle to attain new adaptive roles for the host (exapted TEs). We used phenomics approaches, coupled with reverse genetics, to analyze T-DNA insertion mutants of both previously reported and novel protein-coding exapted TEs in the model plant Arabidopsis thaliana. We show that mutations in most of these exapted TEs result in phenotypes, particularly when challenged by abiotic stress. We built statistical multi-dimensional phenotypic profiles and compared them to wild-type and known stress responsive mutant lines for each particular stress condition. We found that these exapted TEs may play roles in responses to phosphate limitation, tolerance to high salt concentration, freezing temperatures, and arsenic toxicity. These results not only experimentally validate a large set of putative functional exapted TEs recently discovered through computational analysis, but also uncover additional novel phenotypes for previously well-characterized exapted TEs in A. thaliana.
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spelling pubmed-57153672017-12-15 Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements Joly-Lopez, Zoé Forczek, Ewa Vello, Emilio Hoen, Douglas R. Tomita, Akiko Bureau, Thomas E. Front Plant Sci Plant Science Plant phenomics offers unique opportunities to accelerate our understanding of gene function and plant response to different environments, and may be particularly useful for studying previously uncharacterized genes. One important type of poorly characterized genes is those derived from transposable elements (TEs), which have departed from a mobility-driven lifestyle to attain new adaptive roles for the host (exapted TEs). We used phenomics approaches, coupled with reverse genetics, to analyze T-DNA insertion mutants of both previously reported and novel protein-coding exapted TEs in the model plant Arabidopsis thaliana. We show that mutations in most of these exapted TEs result in phenotypes, particularly when challenged by abiotic stress. We built statistical multi-dimensional phenotypic profiles and compared them to wild-type and known stress responsive mutant lines for each particular stress condition. We found that these exapted TEs may play roles in responses to phosphate limitation, tolerance to high salt concentration, freezing temperatures, and arsenic toxicity. These results not only experimentally validate a large set of putative functional exapted TEs recently discovered through computational analysis, but also uncover additional novel phenotypes for previously well-characterized exapted TEs in A. thaliana. Frontiers Media S.A. 2017-11-28 /pmc/articles/PMC5715367/ /pubmed/29250089 http://dx.doi.org/10.3389/fpls.2017.02027 Text en Copyright © 2017 Joly-Lopez, Forczek, Vello, Hoen, Tomita and Bureau. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Joly-Lopez, Zoé
Forczek, Ewa
Vello, Emilio
Hoen, Douglas R.
Tomita, Akiko
Bureau, Thomas E.
Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements
title Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements
title_full Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements
title_fullStr Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements
title_full_unstemmed Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements
title_short Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements
title_sort abiotic stress phenotypes are associated with conserved genes derived from transposable elements
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715367/
https://www.ncbi.nlm.nih.gov/pubmed/29250089
http://dx.doi.org/10.3389/fpls.2017.02027
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