Cargando…

Transcriptomic analysis of wound xylem formation in Pinus canariensis

BACKGROUND: Woody plants, especially trees, usually must face several injuries caused by different agents during their lives. Healing of injuries in stem and branches, affecting the vascular cambium and xylem can take several years. In conifers, healing takes place mainly from the remaining vascular...

Descripción completa

Detalles Bibliográficos
Autores principales: Chano, V., Collada, C., Soto, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715621/
https://www.ncbi.nlm.nih.gov/pubmed/29202766
http://dx.doi.org/10.1186/s12870-017-1183-3
_version_ 1783283802701824000
author Chano, V.
Collada, C.
Soto, A.
author_facet Chano, V.
Collada, C.
Soto, A.
author_sort Chano, V.
collection PubMed
description BACKGROUND: Woody plants, especially trees, usually must face several injuries caused by different agents during their lives. Healing of injuries in stem and branches, affecting the vascular cambium and xylem can take several years. In conifers, healing takes place mainly from the remaining vascular cambium in the margin of the wound. The woundwood formed in conifers during healing usually presents malformed and disordered tracheids as well as abundant traumatic resin ducts. These characteristics affect its functionality as water conductor and its technological properties. RESULTS: In this work we analyze for the first time the transcriptomic basis of the formation of traumatic wood in conifers, and reveal some differences with normal early- and late-wood. Microarray analysis of the differentiating traumatic wood, confirmed by quantitative RT-PCR, has revealed alterations in the transcription profile of up to 1408 genes during the first period of healing. We have grouped these genes in twelve clusters, according to their transcription profiles, and have distinguished accordingly two main phases during this first healing. CONCLUSIONS: Wounding induces a complete rearrangement of the transcriptional program in the cambial zone close to the injuries. At the first instance, radial growth is stopped, and a complete set of defensive genes, mostly related to biotic stress, are induced. Later on, cambial activity is restored in the lateral borders of the wound, even at a high rate. During this second stage certain genes related to early-wood formation, including genes involved in cell wall formation and transcription factors, are significantly overexpressed, while certain late-wood related genes are repressed. Additionally, significant alterations in the transcription profile of abundant non annotated genes are reported. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-017-1183-3) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5715621
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-57156212017-12-08 Transcriptomic analysis of wound xylem formation in Pinus canariensis Chano, V. Collada, C. Soto, A. BMC Plant Biol Research Article BACKGROUND: Woody plants, especially trees, usually must face several injuries caused by different agents during their lives. Healing of injuries in stem and branches, affecting the vascular cambium and xylem can take several years. In conifers, healing takes place mainly from the remaining vascular cambium in the margin of the wound. The woundwood formed in conifers during healing usually presents malformed and disordered tracheids as well as abundant traumatic resin ducts. These characteristics affect its functionality as water conductor and its technological properties. RESULTS: In this work we analyze for the first time the transcriptomic basis of the formation of traumatic wood in conifers, and reveal some differences with normal early- and late-wood. Microarray analysis of the differentiating traumatic wood, confirmed by quantitative RT-PCR, has revealed alterations in the transcription profile of up to 1408 genes during the first period of healing. We have grouped these genes in twelve clusters, according to their transcription profiles, and have distinguished accordingly two main phases during this first healing. CONCLUSIONS: Wounding induces a complete rearrangement of the transcriptional program in the cambial zone close to the injuries. At the first instance, radial growth is stopped, and a complete set of defensive genes, mostly related to biotic stress, are induced. Later on, cambial activity is restored in the lateral borders of the wound, even at a high rate. During this second stage certain genes related to early-wood formation, including genes involved in cell wall formation and transcription factors, are significantly overexpressed, while certain late-wood related genes are repressed. Additionally, significant alterations in the transcription profile of abundant non annotated genes are reported. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-017-1183-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-04 /pmc/articles/PMC5715621/ /pubmed/29202766 http://dx.doi.org/10.1186/s12870-017-1183-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Chano, V.
Collada, C.
Soto, A.
Transcriptomic analysis of wound xylem formation in Pinus canariensis
title Transcriptomic analysis of wound xylem formation in Pinus canariensis
title_full Transcriptomic analysis of wound xylem formation in Pinus canariensis
title_fullStr Transcriptomic analysis of wound xylem formation in Pinus canariensis
title_full_unstemmed Transcriptomic analysis of wound xylem formation in Pinus canariensis
title_short Transcriptomic analysis of wound xylem formation in Pinus canariensis
title_sort transcriptomic analysis of wound xylem formation in pinus canariensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715621/
https://www.ncbi.nlm.nih.gov/pubmed/29202766
http://dx.doi.org/10.1186/s12870-017-1183-3
work_keys_str_mv AT chanov transcriptomicanalysisofwoundxylemformationinpinuscanariensis
AT colladac transcriptomicanalysisofwoundxylemformationinpinuscanariensis
AT sotoa transcriptomicanalysisofwoundxylemformationinpinuscanariensis