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Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development

The ability of Candida albicans to form biofilms is a virulence factor that allows tissue attachment and subsequent infection of host tissues. Fungal biofilms have been particularly well studied, however the vast majority of these studies have been conducted under static conditions. Oral biofilms fo...

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Detalles Bibliográficos
Autores principales: McCall, Andrew, Edgerton, Mira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715965/
https://www.ncbi.nlm.nih.gov/pubmed/29371532
http://dx.doi.org/10.3390/jof3010013
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author McCall, Andrew
Edgerton, Mira
author_facet McCall, Andrew
Edgerton, Mira
author_sort McCall, Andrew
collection PubMed
description The ability of Candida albicans to form biofilms is a virulence factor that allows tissue attachment and subsequent infection of host tissues. Fungal biofilms have been particularly well studied, however the vast majority of these studies have been conducted under static conditions. Oral biofilms form in the presence of salivary flow, therefore we developed a novel flow system used for real-time imaging of fungal biofilm development. C. albicans wild-type (WT) cells readily attached to the substrate surface during the 2 h attachment phase, then formed heterogeneous biofilms after 18 h flow. Quantitative values for biomass, rates of attachment and detachment, and cell–cell adhesion events were obtained for C. albicans WT cells and for a hyperfilamentous mutant Δhog1. Attachment rates of C. albicans WT cells were nearly 2-fold higher than C. albicans Δhog1 cells, although Δhog1 cells formed 4-fold higher biomass. The reduced normalized detachment rate was the primary factor responsible for the increased biomass of Δhog1 biofilm, showing that cell detachment rates are an important predictor for ultimate biofilm mass under flow. Unlike static biofilms, C. albicans cells under constant laminar flow undergo continuous detachment and seeding that may be more representative of the development of in vivo biofilms.
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spelling pubmed-57159652018-01-19 Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development McCall, Andrew Edgerton, Mira J Fungi (Basel) Article The ability of Candida albicans to form biofilms is a virulence factor that allows tissue attachment and subsequent infection of host tissues. Fungal biofilms have been particularly well studied, however the vast majority of these studies have been conducted under static conditions. Oral biofilms form in the presence of salivary flow, therefore we developed a novel flow system used for real-time imaging of fungal biofilm development. C. albicans wild-type (WT) cells readily attached to the substrate surface during the 2 h attachment phase, then formed heterogeneous biofilms after 18 h flow. Quantitative values for biomass, rates of attachment and detachment, and cell–cell adhesion events were obtained for C. albicans WT cells and for a hyperfilamentous mutant Δhog1. Attachment rates of C. albicans WT cells were nearly 2-fold higher than C. albicans Δhog1 cells, although Δhog1 cells formed 4-fold higher biomass. The reduced normalized detachment rate was the primary factor responsible for the increased biomass of Δhog1 biofilm, showing that cell detachment rates are an important predictor for ultimate biofilm mass under flow. Unlike static biofilms, C. albicans cells under constant laminar flow undergo continuous detachment and seeding that may be more representative of the development of in vivo biofilms. MDPI 2017-03-06 /pmc/articles/PMC5715965/ /pubmed/29371532 http://dx.doi.org/10.3390/jof3010013 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
McCall, Andrew
Edgerton, Mira
Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development
title Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development
title_full Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development
title_fullStr Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development
title_full_unstemmed Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development
title_short Real-Time Approach to Flow Cell Imaging of Candida albicans Biofilm Development
title_sort real-time approach to flow cell imaging of candida albicans biofilm development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715965/
https://www.ncbi.nlm.nih.gov/pubmed/29371532
http://dx.doi.org/10.3390/jof3010013
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