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Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study

BACKGROUND: Despite the several strategies available for the management of biofilm-associated ventilator-associated pneumonia, data regarding the efficacy of applying antibiotics to the subglottic space (SS) are scarce. We created an in vitro model to assess the efficacy of antibiotic lock therapy (...

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Autores principales: Pérez-Granda, María Jesús, Latorre, María Consuelo, Alonso, Beatriz, Hortal, Javier, Samaniego, Rafael, Bouza, Emilio, Guembe, María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715999/
https://www.ncbi.nlm.nih.gov/pubmed/29202722
http://dx.doi.org/10.1186/s12879-017-2856-0
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author Pérez-Granda, María Jesús
Latorre, María Consuelo
Alonso, Beatriz
Hortal, Javier
Samaniego, Rafael
Bouza, Emilio
Guembe, María
author_facet Pérez-Granda, María Jesús
Latorre, María Consuelo
Alonso, Beatriz
Hortal, Javier
Samaniego, Rafael
Bouza, Emilio
Guembe, María
author_sort Pérez-Granda, María Jesús
collection PubMed
description BACKGROUND: Despite the several strategies available for the management of biofilm-associated ventilator-associated pneumonia, data regarding the efficacy of applying antibiotics to the subglottic space (SS) are scarce. We created an in vitro model to assess the efficacy of antibiotic lock therapy (ALT) applied in the SS for eradication of Pseudomonas aeruginosa biofilm in endotracheal tubes (ETTs). METHODS: We applied 2 h of ALT to a P. aeruginosa biofilm in ETTs using a single dose (SD) and a 5-day therapy model (5D). We used sterile saline lock therapy (SLT) as the positive control. We compared colony count and the percentage of live cells between both models. RESULTS: The median (IQR) cfu counts/ml and percentage of live cells in the SD-ALT and SD-SLT groups were, respectively, 3.12 × 10(5) (9.7 × 10(4)-0) vs. 8.16 × 10(7) (7.0 × 10(7)-0) (p = 0.05) and 53.2% (50.9%-57.2%) vs. 91.5% (87.3%-93.9%) (p < 0.001). The median (IQR) cfu counts/ml and percentage of live cells in the 5D-ALT and 5D-SLT groups were, respectively, 0 (0-0) vs. 3.2 × 10(7) (2.32 × 10(7)-0) (p = 0.03) and 40.6% (36.6%-60.0%) vs. 90.3% (84.8%-93.9%) (p < 0.001). CONCLUSION: We demonstrated a statistically significant decrease in the viability of P. aeruginosa biofilm after application of 5D-ALT in the SS. Future clinical studies to assess ALT in patients under mechanical ventilation are needed.
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spelling pubmed-57159992017-12-08 Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study Pérez-Granda, María Jesús Latorre, María Consuelo Alonso, Beatriz Hortal, Javier Samaniego, Rafael Bouza, Emilio Guembe, María BMC Infect Dis Research Article BACKGROUND: Despite the several strategies available for the management of biofilm-associated ventilator-associated pneumonia, data regarding the efficacy of applying antibiotics to the subglottic space (SS) are scarce. We created an in vitro model to assess the efficacy of antibiotic lock therapy (ALT) applied in the SS for eradication of Pseudomonas aeruginosa biofilm in endotracheal tubes (ETTs). METHODS: We applied 2 h of ALT to a P. aeruginosa biofilm in ETTs using a single dose (SD) and a 5-day therapy model (5D). We used sterile saline lock therapy (SLT) as the positive control. We compared colony count and the percentage of live cells between both models. RESULTS: The median (IQR) cfu counts/ml and percentage of live cells in the SD-ALT and SD-SLT groups were, respectively, 3.12 × 10(5) (9.7 × 10(4)-0) vs. 8.16 × 10(7) (7.0 × 10(7)-0) (p = 0.05) and 53.2% (50.9%-57.2%) vs. 91.5% (87.3%-93.9%) (p < 0.001). The median (IQR) cfu counts/ml and percentage of live cells in the 5D-ALT and 5D-SLT groups were, respectively, 0 (0-0) vs. 3.2 × 10(7) (2.32 × 10(7)-0) (p = 0.03) and 40.6% (36.6%-60.0%) vs. 90.3% (84.8%-93.9%) (p < 0.001). CONCLUSION: We demonstrated a statistically significant decrease in the viability of P. aeruginosa biofilm after application of 5D-ALT in the SS. Future clinical studies to assess ALT in patients under mechanical ventilation are needed. BioMed Central 2017-12-04 /pmc/articles/PMC5715999/ /pubmed/29202722 http://dx.doi.org/10.1186/s12879-017-2856-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Pérez-Granda, María Jesús
Latorre, María Consuelo
Alonso, Beatriz
Hortal, Javier
Samaniego, Rafael
Bouza, Emilio
Guembe, María
Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study
title Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study
title_full Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study
title_fullStr Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study
title_full_unstemmed Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study
title_short Eradication of P. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study
title_sort eradication of p. aeruginosa biofilm in endotracheal tubes based on lock therapy: results from an in vitro study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715999/
https://www.ncbi.nlm.nih.gov/pubmed/29202722
http://dx.doi.org/10.1186/s12879-017-2856-0
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