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Identification and characterization of T reg–like cells in zebrafish
Regulatory T (T reg) cells are a specialized sublineage of T lymphocytes that suppress autoreactive T cells. Functional studies of T reg cells in vitro have defined multiple suppression mechanisms, and studies of T reg–deficient humans and mice have made clear the important role that these cells pla...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716030/ https://www.ncbi.nlm.nih.gov/pubmed/29066577 http://dx.doi.org/10.1084/jem.20162084 |
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author | Kasheta, Melissa Painter, Corrie A. Moore, Finola E. Lobbardi, Riadh Bryll, Alysia Freiman, Eli Stachura, David Rogers, Arlin B. Houvras, Yariv Langenau, David M. Ceol, Craig J. |
author_facet | Kasheta, Melissa Painter, Corrie A. Moore, Finola E. Lobbardi, Riadh Bryll, Alysia Freiman, Eli Stachura, David Rogers, Arlin B. Houvras, Yariv Langenau, David M. Ceol, Craig J. |
author_sort | Kasheta, Melissa |
collection | PubMed |
description | Regulatory T (T reg) cells are a specialized sublineage of T lymphocytes that suppress autoreactive T cells. Functional studies of T reg cells in vitro have defined multiple suppression mechanisms, and studies of T reg–deficient humans and mice have made clear the important role that these cells play in preventing autoimmunity. However, many questions remain about how T reg cells act in vivo. Specifically, it is not clear which suppression mechanisms are most important, where T reg cells act, and how they get there. To begin to address these issues, we sought to identify T reg cells in zebrafish, a model system that provides unparalleled advantages in live-cell imaging and high-throughput genetic analyses. Using a FOXP3 orthologue as a marker, we identified CD4-enriched, mature T lymphocytes with properties of T reg cells. Zebrafish mutant for foxp3a displayed excess T lymphocytes, splenomegaly, and a profound inflammatory phenotype that was suppressed by genetic ablation of lymphocytes. This study identifies T reg–like cells in zebrafish, providing both a model to study the normal functions of these cells in vivo and mutants to explore the consequences of their loss. |
format | Online Article Text |
id | pubmed-5716030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-57160302018-06-04 Identification and characterization of T reg–like cells in zebrafish Kasheta, Melissa Painter, Corrie A. Moore, Finola E. Lobbardi, Riadh Bryll, Alysia Freiman, Eli Stachura, David Rogers, Arlin B. Houvras, Yariv Langenau, David M. Ceol, Craig J. J Exp Med Research Articles Regulatory T (T reg) cells are a specialized sublineage of T lymphocytes that suppress autoreactive T cells. Functional studies of T reg cells in vitro have defined multiple suppression mechanisms, and studies of T reg–deficient humans and mice have made clear the important role that these cells play in preventing autoimmunity. However, many questions remain about how T reg cells act in vivo. Specifically, it is not clear which suppression mechanisms are most important, where T reg cells act, and how they get there. To begin to address these issues, we sought to identify T reg cells in zebrafish, a model system that provides unparalleled advantages in live-cell imaging and high-throughput genetic analyses. Using a FOXP3 orthologue as a marker, we identified CD4-enriched, mature T lymphocytes with properties of T reg cells. Zebrafish mutant for foxp3a displayed excess T lymphocytes, splenomegaly, and a profound inflammatory phenotype that was suppressed by genetic ablation of lymphocytes. This study identifies T reg–like cells in zebrafish, providing both a model to study the normal functions of these cells in vivo and mutants to explore the consequences of their loss. The Rockefeller University Press 2017-12-04 /pmc/articles/PMC5716030/ /pubmed/29066577 http://dx.doi.org/10.1084/jem.20162084 Text en © 2017 Kasheta et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Kasheta, Melissa Painter, Corrie A. Moore, Finola E. Lobbardi, Riadh Bryll, Alysia Freiman, Eli Stachura, David Rogers, Arlin B. Houvras, Yariv Langenau, David M. Ceol, Craig J. Identification and characterization of T reg–like cells in zebrafish |
title | Identification and characterization of T reg–like cells in zebrafish |
title_full | Identification and characterization of T reg–like cells in zebrafish |
title_fullStr | Identification and characterization of T reg–like cells in zebrafish |
title_full_unstemmed | Identification and characterization of T reg–like cells in zebrafish |
title_short | Identification and characterization of T reg–like cells in zebrafish |
title_sort | identification and characterization of t reg–like cells in zebrafish |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716030/ https://www.ncbi.nlm.nih.gov/pubmed/29066577 http://dx.doi.org/10.1084/jem.20162084 |
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