Cargando…

Identification and characterization of T reg–like cells in zebrafish

Regulatory T (T reg) cells are a specialized sublineage of T lymphocytes that suppress autoreactive T cells. Functional studies of T reg cells in vitro have defined multiple suppression mechanisms, and studies of T reg–deficient humans and mice have made clear the important role that these cells pla...

Descripción completa

Detalles Bibliográficos
Autores principales: Kasheta, Melissa, Painter, Corrie A., Moore, Finola E., Lobbardi, Riadh, Bryll, Alysia, Freiman, Eli, Stachura, David, Rogers, Arlin B., Houvras, Yariv, Langenau, David M., Ceol, Craig J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716030/
https://www.ncbi.nlm.nih.gov/pubmed/29066577
http://dx.doi.org/10.1084/jem.20162084
_version_ 1783283863522377728
author Kasheta, Melissa
Painter, Corrie A.
Moore, Finola E.
Lobbardi, Riadh
Bryll, Alysia
Freiman, Eli
Stachura, David
Rogers, Arlin B.
Houvras, Yariv
Langenau, David M.
Ceol, Craig J.
author_facet Kasheta, Melissa
Painter, Corrie A.
Moore, Finola E.
Lobbardi, Riadh
Bryll, Alysia
Freiman, Eli
Stachura, David
Rogers, Arlin B.
Houvras, Yariv
Langenau, David M.
Ceol, Craig J.
author_sort Kasheta, Melissa
collection PubMed
description Regulatory T (T reg) cells are a specialized sublineage of T lymphocytes that suppress autoreactive T cells. Functional studies of T reg cells in vitro have defined multiple suppression mechanisms, and studies of T reg–deficient humans and mice have made clear the important role that these cells play in preventing autoimmunity. However, many questions remain about how T reg cells act in vivo. Specifically, it is not clear which suppression mechanisms are most important, where T reg cells act, and how they get there. To begin to address these issues, we sought to identify T reg cells in zebrafish, a model system that provides unparalleled advantages in live-cell imaging and high-throughput genetic analyses. Using a FOXP3 orthologue as a marker, we identified CD4-enriched, mature T lymphocytes with properties of T reg cells. Zebrafish mutant for foxp3a displayed excess T lymphocytes, splenomegaly, and a profound inflammatory phenotype that was suppressed by genetic ablation of lymphocytes. This study identifies T reg–like cells in zebrafish, providing both a model to study the normal functions of these cells in vivo and mutants to explore the consequences of their loss.
format Online
Article
Text
id pubmed-5716030
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-57160302018-06-04 Identification and characterization of T reg–like cells in zebrafish Kasheta, Melissa Painter, Corrie A. Moore, Finola E. Lobbardi, Riadh Bryll, Alysia Freiman, Eli Stachura, David Rogers, Arlin B. Houvras, Yariv Langenau, David M. Ceol, Craig J. J Exp Med Research Articles Regulatory T (T reg) cells are a specialized sublineage of T lymphocytes that suppress autoreactive T cells. Functional studies of T reg cells in vitro have defined multiple suppression mechanisms, and studies of T reg–deficient humans and mice have made clear the important role that these cells play in preventing autoimmunity. However, many questions remain about how T reg cells act in vivo. Specifically, it is not clear which suppression mechanisms are most important, where T reg cells act, and how they get there. To begin to address these issues, we sought to identify T reg cells in zebrafish, a model system that provides unparalleled advantages in live-cell imaging and high-throughput genetic analyses. Using a FOXP3 orthologue as a marker, we identified CD4-enriched, mature T lymphocytes with properties of T reg cells. Zebrafish mutant for foxp3a displayed excess T lymphocytes, splenomegaly, and a profound inflammatory phenotype that was suppressed by genetic ablation of lymphocytes. This study identifies T reg–like cells in zebrafish, providing both a model to study the normal functions of these cells in vivo and mutants to explore the consequences of their loss. The Rockefeller University Press 2017-12-04 /pmc/articles/PMC5716030/ /pubmed/29066577 http://dx.doi.org/10.1084/jem.20162084 Text en © 2017 Kasheta et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Kasheta, Melissa
Painter, Corrie A.
Moore, Finola E.
Lobbardi, Riadh
Bryll, Alysia
Freiman, Eli
Stachura, David
Rogers, Arlin B.
Houvras, Yariv
Langenau, David M.
Ceol, Craig J.
Identification and characterization of T reg–like cells in zebrafish
title Identification and characterization of T reg–like cells in zebrafish
title_full Identification and characterization of T reg–like cells in zebrafish
title_fullStr Identification and characterization of T reg–like cells in zebrafish
title_full_unstemmed Identification and characterization of T reg–like cells in zebrafish
title_short Identification and characterization of T reg–like cells in zebrafish
title_sort identification and characterization of t reg–like cells in zebrafish
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716030/
https://www.ncbi.nlm.nih.gov/pubmed/29066577
http://dx.doi.org/10.1084/jem.20162084
work_keys_str_mv AT kashetamelissa identificationandcharacterizationoftreglikecellsinzebrafish
AT paintercorriea identificationandcharacterizationoftreglikecellsinzebrafish
AT moorefinolae identificationandcharacterizationoftreglikecellsinzebrafish
AT lobbardiriadh identificationandcharacterizationoftreglikecellsinzebrafish
AT bryllalysia identificationandcharacterizationoftreglikecellsinzebrafish
AT freimaneli identificationandcharacterizationoftreglikecellsinzebrafish
AT stachuradavid identificationandcharacterizationoftreglikecellsinzebrafish
AT rogersarlinb identificationandcharacterizationoftreglikecellsinzebrafish
AT houvrasyariv identificationandcharacterizationoftreglikecellsinzebrafish
AT langenaudavidm identificationandcharacterizationoftreglikecellsinzebrafish
AT ceolcraigj identificationandcharacterizationoftreglikecellsinzebrafish