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An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers

Mouse embryonic stem (ES) cells are a popular model system to study biological processes, though uncovering recessive phenotypes requires inactivating both alleles. Building upon resources from the International Knockout Mouse Consortium (IKMC), we developed a targeting vector for second allele inac...

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Detalles Bibliográficos
Autores principales:	Fisher, Cynthia L., Marks, Hendrik, Cho, Lily Ting-yin, Andrews, Robert, Wormald, Sam, Carroll, Thomas, Iyer, Vivek, Tate, Peri, Rosen, Barry, Stunnenberg, Hendrik G., Fisher, Amanda G., Skarnes, William C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716182/ https://www.ncbi.nlm.nih.gov/pubmed/28981838 http://dx.doi.org/10.1093/nar/gkx811

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