Cargando…
Rice NAD(+)-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes
Sirtuins, a family of proteins with homology to the yeast silent information regulator 2 (Sir2), are NAD(+)-dependent histone deacetylases and play crucial roles in energy sensing and regulation in yeast and animal cells. Plants are autotrophic organisms and display distinct features of carbon and e...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716216/ https://www.ncbi.nlm.nih.gov/pubmed/28981755 http://dx.doi.org/10.1093/nar/gkx825 |
_version_ | 1783283901904453632 |
---|---|
author | Zhang, Hua Zhao, Yu Zhou, Dao-Xiu |
author_facet | Zhang, Hua Zhao, Yu Zhou, Dao-Xiu |
author_sort | Zhang, Hua |
collection | PubMed |
description | Sirtuins, a family of proteins with homology to the yeast silent information regulator 2 (Sir2), are NAD(+)-dependent histone deacetylases and play crucial roles in energy sensing and regulation in yeast and animal cells. Plants are autotrophic organisms and display distinct features of carbon and energy metabolism. It remains largely unexplored whether and how plant cells sense energy/redox status to control carbon metabolic flux under various growth conditions. In this work, we show that the rice nuclear sirtuin OsSRT1 not only functions as an epigenetic regulator to repress glycolytic genes expression and glycolysis in seedlings, but also inhibits transcriptional activity of glyceraldehyde-3-phosphatedehydrogenase (GAPDH) that is enriched on glycolytic genes promoters and stimulates their expression. We show that OsSRT1 reduces GAPDH lysine acetylation and nuclear accumulation that are enhanced by oxidative stress. Mass spectrometry identified six acetylated lysines regulated by OsSRT1. OsSRT1-dependent lysine deacetylation of OsGAPDH1 represses transcriptional activity of the protein. The results indicate that OsSRT1 represses glycolysis by both regulating epigenetic modification of histone and inhibiting the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes in rice. |
format | Online Article Text |
id | pubmed-5716216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-57162162020-08-18 Rice NAD(+)-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes Zhang, Hua Zhao, Yu Zhou, Dao-Xiu Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Sirtuins, a family of proteins with homology to the yeast silent information regulator 2 (Sir2), are NAD(+)-dependent histone deacetylases and play crucial roles in energy sensing and regulation in yeast and animal cells. Plants are autotrophic organisms and display distinct features of carbon and energy metabolism. It remains largely unexplored whether and how plant cells sense energy/redox status to control carbon metabolic flux under various growth conditions. In this work, we show that the rice nuclear sirtuin OsSRT1 not only functions as an epigenetic regulator to repress glycolytic genes expression and glycolysis in seedlings, but also inhibits transcriptional activity of glyceraldehyde-3-phosphatedehydrogenase (GAPDH) that is enriched on glycolytic genes promoters and stimulates their expression. We show that OsSRT1 reduces GAPDH lysine acetylation and nuclear accumulation that are enhanced by oxidative stress. Mass spectrometry identified six acetylated lysines regulated by OsSRT1. OsSRT1-dependent lysine deacetylation of OsGAPDH1 represses transcriptional activity of the protein. The results indicate that OsSRT1 represses glycolysis by both regulating epigenetic modification of histone and inhibiting the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes in rice. Oxford University Press 2017-12-01 2017-09-13 /pmc/articles/PMC5716216/ /pubmed/28981755 http://dx.doi.org/10.1093/nar/gkx825 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Gene regulation, Chromatin and Epigenetics Zhang, Hua Zhao, Yu Zhou, Dao-Xiu Rice NAD(+)-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes |
title | Rice NAD(+)-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes |
title_full | Rice NAD(+)-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes |
title_fullStr | Rice NAD(+)-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes |
title_full_unstemmed | Rice NAD(+)-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes |
title_short | Rice NAD(+)-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes |
title_sort | rice nad(+)-dependent histone deacetylase ossrt1 represses glycolysis and regulates the moonlighting function of gapdh as a transcriptional activator of glycolytic genes |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716216/ https://www.ncbi.nlm.nih.gov/pubmed/28981755 http://dx.doi.org/10.1093/nar/gkx825 |
work_keys_str_mv | AT zhanghua ricenaddependenthistonedeacetylaseossrt1repressesglycolysisandregulatesthemoonlightingfunctionofgapdhasatranscriptionalactivatorofglycolyticgenes AT zhaoyu ricenaddependenthistonedeacetylaseossrt1repressesglycolysisandregulatesthemoonlightingfunctionofgapdhasatranscriptionalactivatorofglycolyticgenes AT zhoudaoxiu ricenaddependenthistonedeacetylaseossrt1repressesglycolysisandregulatesthemoonlightingfunctionofgapdhasatranscriptionalactivatorofglycolyticgenes |