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Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells
OBJECTIVE: Caveolin-3 (CAV3) protein is known to be expressed specifically in various myocytes, but its physiological function remains unclear. CAV3, located at the cell membrane, may promote the sensitivity of the Akt signaling pathway, which is closely related to glucose metabolism and to cell gro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716543/ https://www.ncbi.nlm.nih.gov/pubmed/29206848 http://dx.doi.org/10.1371/journal.pone.0189004 |
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author | Shang, Lina Chen, Tingting Deng, Yufeng Huang, Yiyuan Huang, Yuanheng Xian, Jing Lu, Wensheng Yang, Lihui Huang, Qin |
author_facet | Shang, Lina Chen, Tingting Deng, Yufeng Huang, Yiyuan Huang, Yuanheng Xian, Jing Lu, Wensheng Yang, Lihui Huang, Qin |
author_sort | Shang, Lina |
collection | PubMed |
description | OBJECTIVE: Caveolin-3 (CAV3) protein is known to be expressed specifically in various myocytes, but its physiological function remains unclear. CAV3, located at the cell membrane, may promote the sensitivity of the Akt signaling pathway, which is closely related to glucose metabolism and to cell growth and proliferation. METHODS: The CAV3 gene was stably transfected into C2C12 muscle cells, and the effects were evaluated by biochemical assays, WB and confocal microscopy for the observation of cellular glucose metabolism, growth and proliferation, and the effect of CAV3 on the Akt signaling pathway with no insulin stimulation. RESULTS: After C2C12 cells were transfected with the mouse CAV3 gene, which increased CAV3 expression, the abundance of the CAV3 and GLUT4 proteins on the cell membrane increased, but the total GLUT4 protein content of the cell was unchanged. Glucose uptake was increased, and this did not affect the glycogen synthesis, but the cell surface area and cell proliferation increased. While there were significant increases in p-Akt and p-p70s6K, which is a downstream component of Akt signaling, the level of GSK3β protein, another component of Akt signaling did not change. CONCLUSIONS: The muscle, CAV3 protein can activate Akt signaling, increase GLUT4 protein localization in the cell membrane, increase glucose uptake, and promote myocyte growth and proliferation. CAV3 protein has a physiological role in glycometabolism, growth and proliferation, independent of insulin stimulation. |
format | Online Article Text |
id | pubmed-5716543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57165432017-12-15 Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells Shang, Lina Chen, Tingting Deng, Yufeng Huang, Yiyuan Huang, Yuanheng Xian, Jing Lu, Wensheng Yang, Lihui Huang, Qin PLoS One Research Article OBJECTIVE: Caveolin-3 (CAV3) protein is known to be expressed specifically in various myocytes, but its physiological function remains unclear. CAV3, located at the cell membrane, may promote the sensitivity of the Akt signaling pathway, which is closely related to glucose metabolism and to cell growth and proliferation. METHODS: The CAV3 gene was stably transfected into C2C12 muscle cells, and the effects were evaluated by biochemical assays, WB and confocal microscopy for the observation of cellular glucose metabolism, growth and proliferation, and the effect of CAV3 on the Akt signaling pathway with no insulin stimulation. RESULTS: After C2C12 cells were transfected with the mouse CAV3 gene, which increased CAV3 expression, the abundance of the CAV3 and GLUT4 proteins on the cell membrane increased, but the total GLUT4 protein content of the cell was unchanged. Glucose uptake was increased, and this did not affect the glycogen synthesis, but the cell surface area and cell proliferation increased. While there were significant increases in p-Akt and p-p70s6K, which is a downstream component of Akt signaling, the level of GSK3β protein, another component of Akt signaling did not change. CONCLUSIONS: The muscle, CAV3 protein can activate Akt signaling, increase GLUT4 protein localization in the cell membrane, increase glucose uptake, and promote myocyte growth and proliferation. CAV3 protein has a physiological role in glycometabolism, growth and proliferation, independent of insulin stimulation. Public Library of Science 2017-12-05 /pmc/articles/PMC5716543/ /pubmed/29206848 http://dx.doi.org/10.1371/journal.pone.0189004 Text en © 2017 Shang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shang, Lina Chen, Tingting Deng, Yufeng Huang, Yiyuan Huang, Yuanheng Xian, Jing Lu, Wensheng Yang, Lihui Huang, Qin Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells |
title | Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells |
title_full | Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells |
title_fullStr | Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells |
title_full_unstemmed | Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells |
title_short | Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells |
title_sort | caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716543/ https://www.ncbi.nlm.nih.gov/pubmed/29206848 http://dx.doi.org/10.1371/journal.pone.0189004 |
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