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The human kidney capsule contains a functionally distinct mesenchymal stromal cell population

We recently demonstrated that the adult human kidney cortex contains a perivascular stromal cell (kPSC) that shows organotypic properties and is important for repair and stabilisation of kidney function. Not only the kidney cortex but also the kidney capsule contains stromal cells that are important...

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Autores principales: Leuning, Daniëlle G., Engelse, Marten A., Lievers, Ellen, Bijkerk, Roel, Reinders, Marlies E. J., de Boer, Hetty C., van Kooten, Cees, Rabelink, Ton J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716605/
https://www.ncbi.nlm.nih.gov/pubmed/29206835
http://dx.doi.org/10.1371/journal.pone.0187118
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author Leuning, Daniëlle G.
Engelse, Marten A.
Lievers, Ellen
Bijkerk, Roel
Reinders, Marlies E. J.
de Boer, Hetty C.
van Kooten, Cees
Rabelink, Ton J.
author_facet Leuning, Daniëlle G.
Engelse, Marten A.
Lievers, Ellen
Bijkerk, Roel
Reinders, Marlies E. J.
de Boer, Hetty C.
van Kooten, Cees
Rabelink, Ton J.
author_sort Leuning, Daniëlle G.
collection PubMed
description We recently demonstrated that the adult human kidney cortex contains a perivascular stromal cell (kPSC) that shows organotypic properties and is important for repair and stabilisation of kidney function. Not only the kidney cortex but also the kidney capsule contains stromal cells that are important for the three dimensional organisation of the kidney during nephrogenesis. They provide the barrier function of the capsule which is critical for homeostatic processes such as pressure natriuresis. We postulated that stromal cells derived from the kidney capsule may therefore also have specific properties and functions. To this end, we isolated these capsule mesenchymal stromal cells (cMSC) from human cadaveric kidneys that were not suitable for transplantation. There were several similarities between cMSCs and kPSCs including support of vascular plexus formation, phenotypic marker expression and resistance against myofibroblast transformation. However, compared to kPSCs, cMSCs showed distinct mRNA and miRNA expression profiles, showed increased immunosuppressive capacity, and displayed strongly reduced HGF production, contributing to the inability to enhance kidney epithelial repair. Therefore cMSCs are a distinct, novel human kidney-derived MSC-population and these data underpin the large functional diversity of phenotypic similar stromal cells in relation to their anatomic site, even within one organ.
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spelling pubmed-57166052017-12-15 The human kidney capsule contains a functionally distinct mesenchymal stromal cell population Leuning, Daniëlle G. Engelse, Marten A. Lievers, Ellen Bijkerk, Roel Reinders, Marlies E. J. de Boer, Hetty C. van Kooten, Cees Rabelink, Ton J. PLoS One Research Article We recently demonstrated that the adult human kidney cortex contains a perivascular stromal cell (kPSC) that shows organotypic properties and is important for repair and stabilisation of kidney function. Not only the kidney cortex but also the kidney capsule contains stromal cells that are important for the three dimensional organisation of the kidney during nephrogenesis. They provide the barrier function of the capsule which is critical for homeostatic processes such as pressure natriuresis. We postulated that stromal cells derived from the kidney capsule may therefore also have specific properties and functions. To this end, we isolated these capsule mesenchymal stromal cells (cMSC) from human cadaveric kidneys that were not suitable for transplantation. There were several similarities between cMSCs and kPSCs including support of vascular plexus formation, phenotypic marker expression and resistance against myofibroblast transformation. However, compared to kPSCs, cMSCs showed distinct mRNA and miRNA expression profiles, showed increased immunosuppressive capacity, and displayed strongly reduced HGF production, contributing to the inability to enhance kidney epithelial repair. Therefore cMSCs are a distinct, novel human kidney-derived MSC-population and these data underpin the large functional diversity of phenotypic similar stromal cells in relation to their anatomic site, even within one organ. Public Library of Science 2017-12-05 /pmc/articles/PMC5716605/ /pubmed/29206835 http://dx.doi.org/10.1371/journal.pone.0187118 Text en © 2017 Leuning et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Leuning, Daniëlle G.
Engelse, Marten A.
Lievers, Ellen
Bijkerk, Roel
Reinders, Marlies E. J.
de Boer, Hetty C.
van Kooten, Cees
Rabelink, Ton J.
The human kidney capsule contains a functionally distinct mesenchymal stromal cell population
title The human kidney capsule contains a functionally distinct mesenchymal stromal cell population
title_full The human kidney capsule contains a functionally distinct mesenchymal stromal cell population
title_fullStr The human kidney capsule contains a functionally distinct mesenchymal stromal cell population
title_full_unstemmed The human kidney capsule contains a functionally distinct mesenchymal stromal cell population
title_short The human kidney capsule contains a functionally distinct mesenchymal stromal cell population
title_sort human kidney capsule contains a functionally distinct mesenchymal stromal cell population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716605/
https://www.ncbi.nlm.nih.gov/pubmed/29206835
http://dx.doi.org/10.1371/journal.pone.0187118
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