Cargando…
Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGFβ stimulation in cancer
Transforming growth factor β (TGFβ) is a key regulator of epithelial-to-mesenchymal transition (EMT) during embryogenesis and in tumors. The effect of TGFβ, on ΕΜΤ, is conveyed by induction of the pro-invasive transcription factor Snail1. In this study, we report that TGFβ stimulates Snail1 sumoylat...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716685/ https://www.ncbi.nlm.nih.gov/pubmed/29228645 http://dx.doi.org/10.18632/oncotarget.20097 |
_version_ | 1783284000085770240 |
---|---|
author | Gudey, Shyam Kumar Sundar, Reshma Heldin, Carl-Henrik Bergh, Anders Landström, Marene |
author_facet | Gudey, Shyam Kumar Sundar, Reshma Heldin, Carl-Henrik Bergh, Anders Landström, Marene |
author_sort | Gudey, Shyam Kumar |
collection | PubMed |
description | Transforming growth factor β (TGFβ) is a key regulator of epithelial-to-mesenchymal transition (EMT) during embryogenesis and in tumors. The effect of TGFβ, on ΕΜΤ, is conveyed by induction of the pro-invasive transcription factor Snail1. In this study, we report that TGFβ stimulates Snail1 sumoylation in aggressive prostate, breast and lung cancer cells. Sumoylation of Snail1 lysine residue 234 confers its transcriptional activity, inducing the expression of classical EMT genes, as well as TGFβ receptor I (TβRI) and the transcriptional repressor Hes1. Mutation of Snail1 lysine residue 234 to arginine (K234R) abolished sumoylation of Snail1, as well as its migratory and invasive properties in human prostate cancer cells. An increased immunohistochemical expression of Snail1, Sumo1, TβRI, Hes1, and c-Jun was observed in aggressive prostate cancer tissues, consistent with their functional roles in tumorigenesis. |
format | Online Article Text |
id | pubmed-5716685 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57166852017-12-08 Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGFβ stimulation in cancer Gudey, Shyam Kumar Sundar, Reshma Heldin, Carl-Henrik Bergh, Anders Landström, Marene Oncotarget Priority Research Paper Transforming growth factor β (TGFβ) is a key regulator of epithelial-to-mesenchymal transition (EMT) during embryogenesis and in tumors. The effect of TGFβ, on ΕΜΤ, is conveyed by induction of the pro-invasive transcription factor Snail1. In this study, we report that TGFβ stimulates Snail1 sumoylation in aggressive prostate, breast and lung cancer cells. Sumoylation of Snail1 lysine residue 234 confers its transcriptional activity, inducing the expression of classical EMT genes, as well as TGFβ receptor I (TβRI) and the transcriptional repressor Hes1. Mutation of Snail1 lysine residue 234 to arginine (K234R) abolished sumoylation of Snail1, as well as its migratory and invasive properties in human prostate cancer cells. An increased immunohistochemical expression of Snail1, Sumo1, TβRI, Hes1, and c-Jun was observed in aggressive prostate cancer tissues, consistent with their functional roles in tumorigenesis. Impact Journals LLC 2017-08-09 /pmc/articles/PMC5716685/ /pubmed/29228645 http://dx.doi.org/10.18632/oncotarget.20097 Text en Copyright: © 2017 Gudey et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Priority Research Paper Gudey, Shyam Kumar Sundar, Reshma Heldin, Carl-Henrik Bergh, Anders Landström, Marene Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGFβ stimulation in cancer |
title | Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGFβ stimulation in cancer |
title_full | Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGFβ stimulation in cancer |
title_fullStr | Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGFβ stimulation in cancer |
title_full_unstemmed | Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGFβ stimulation in cancer |
title_short | Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGFβ stimulation in cancer |
title_sort | pro-invasive properties of snail1 are regulated by sumoylation in response to tgfβ stimulation in cancer |
topic | Priority Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5716685/ https://www.ncbi.nlm.nih.gov/pubmed/29228645 http://dx.doi.org/10.18632/oncotarget.20097 |
work_keys_str_mv | AT gudeyshyamkumar proinvasivepropertiesofsnail1areregulatedbysumoylationinresponsetotgfbstimulationincancer AT sundarreshma proinvasivepropertiesofsnail1areregulatedbysumoylationinresponsetotgfbstimulationincancer AT heldincarlhenrik proinvasivepropertiesofsnail1areregulatedbysumoylationinresponsetotgfbstimulationincancer AT berghanders proinvasivepropertiesofsnail1areregulatedbysumoylationinresponsetotgfbstimulationincancer AT landstrommarene proinvasivepropertiesofsnail1areregulatedbysumoylationinresponsetotgfbstimulationincancer |