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Hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice

The utilisation of nanoparticles as the means of targeted delivery of therapeutics and/or imaging agents could greatly enhance the specific transport of biologically active payloads to target tissues while avoiding or reducing undesired side-effects. To allow for this to become a reality, the questi...

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Autores principales: Kermanizadeh, Ali, Jacobsen, Nicklas R., Roursgaard, Martin, Loft, Steffen, Møller, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717320/
https://www.ncbi.nlm.nih.gov/pubmed/29234737
http://dx.doi.org/10.1016/j.heliyon.2017.e00458
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author Kermanizadeh, Ali
Jacobsen, Nicklas R.
Roursgaard, Martin
Loft, Steffen
Møller, Peter
author_facet Kermanizadeh, Ali
Jacobsen, Nicklas R.
Roursgaard, Martin
Loft, Steffen
Møller, Peter
author_sort Kermanizadeh, Ali
collection PubMed
description The utilisation of nanoparticles as the means of targeted delivery of therapeutics and/or imaging agents could greatly enhance the specific transport of biologically active payloads to target tissues while avoiding or reducing undesired side-effects. To allow for this to become a reality, the question of potential toxicological effects needs to be addressed. In the present investigation, a cationic liposome with prospective for medical applications was constructed and thoroughly assessed for any material-induced hepatic adverse effects in vivo − in healthy and alcoholic hepatic disease models and in vitro − (HepG2 cells). The data demonstrated that intravenous injection of liposomes did not cause any significant in vivo hepatic toxicity (inflammation, alterations in blood parameters, anti-oxidant depletion, acute phase response and histopathology) at doses of 200 μg per mouse in either healthy or chronically alcohol fed mice. Additionally, the in vitro material-induced adverse effects (cytotoxicity, inflammation or albumin secretion) were all also minimal. The data from this study demonstrated that the intravenous injection of cationic liposomes does not cause hepatic toxicity. This investigation is important as it investigates the toxicity of a nano-sized material in a model of alcoholic hepatic disease in vitro and in vivo. This is an area of research in the field of nanotoxicology that is currently almost entirely overlooked.
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spelling pubmed-57173202017-12-11 Hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice Kermanizadeh, Ali Jacobsen, Nicklas R. Roursgaard, Martin Loft, Steffen Møller, Peter Heliyon Article The utilisation of nanoparticles as the means of targeted delivery of therapeutics and/or imaging agents could greatly enhance the specific transport of biologically active payloads to target tissues while avoiding or reducing undesired side-effects. To allow for this to become a reality, the question of potential toxicological effects needs to be addressed. In the present investigation, a cationic liposome with prospective for medical applications was constructed and thoroughly assessed for any material-induced hepatic adverse effects in vivo − in healthy and alcoholic hepatic disease models and in vitro − (HepG2 cells). The data demonstrated that intravenous injection of liposomes did not cause any significant in vivo hepatic toxicity (inflammation, alterations in blood parameters, anti-oxidant depletion, acute phase response and histopathology) at doses of 200 μg per mouse in either healthy or chronically alcohol fed mice. Additionally, the in vitro material-induced adverse effects (cytotoxicity, inflammation or albumin secretion) were all also minimal. The data from this study demonstrated that the intravenous injection of cationic liposomes does not cause hepatic toxicity. This investigation is important as it investigates the toxicity of a nano-sized material in a model of alcoholic hepatic disease in vitro and in vivo. This is an area of research in the field of nanotoxicology that is currently almost entirely overlooked. Elsevier 2017-12-01 /pmc/articles/PMC5717320/ /pubmed/29234737 http://dx.doi.org/10.1016/j.heliyon.2017.e00458 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Kermanizadeh, Ali
Jacobsen, Nicklas R.
Roursgaard, Martin
Loft, Steffen
Møller, Peter
Hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice
title Hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice
title_full Hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice
title_fullStr Hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice
title_full_unstemmed Hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice
title_short Hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice
title_sort hepatic toxicity assessment of cationic liposome exposure in healthy and chronic alcohol fed mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717320/
https://www.ncbi.nlm.nih.gov/pubmed/29234737
http://dx.doi.org/10.1016/j.heliyon.2017.e00458
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