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Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin
Bacterial pathogens that colonize host surfaces are subjected to physical stresses such as fluid flow and cell surface contacts. How bacteria respond to such mechanical cues is an important yet poorly understood issue. Staphylococcus aureus uses a repertoire of surface proteins to resist shear stres...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717387/ https://www.ncbi.nlm.nih.gov/pubmed/29208742 http://dx.doi.org/10.1128/mBio.01748-17 |
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author | Vitry, Pauline Valotteau, Claire Feuillie, Cécile Bernard, Simon Alsteens, David Geoghegan, Joan A. Dufrêne, Yves F. |
author_facet | Vitry, Pauline Valotteau, Claire Feuillie, Cécile Bernard, Simon Alsteens, David Geoghegan, Joan A. Dufrêne, Yves F. |
author_sort | Vitry, Pauline |
collection | PubMed |
description | Bacterial pathogens that colonize host surfaces are subjected to physical stresses such as fluid flow and cell surface contacts. How bacteria respond to such mechanical cues is an important yet poorly understood issue. Staphylococcus aureus uses a repertoire of surface proteins to resist shear stress during the colonization of host tissues, but whether their adhesive functions can be modulated by physical forces is not known. Here, we show that the interaction of S. aureus clumping factor B (ClfB) with the squamous epithelial cell envelope protein loricrin is enhanced by mechanical force. We find that ClfB mediates S. aureus adhesion to loricrin through weak and strong molecular interactions both in a laboratory strain and in a clinical isolate. Strong forces (~1,500 pN), among the strongest measured for a receptor-ligand bond, are consistent with a high-affinity “dock, lock, and latch” binding mechanism involving dynamic conformational changes in the adhesin. Notably, we demonstrate that the strength of the ClfB-loricrin bond increases as mechanical force is applied. These findings favor a two-state model whereby bacterial adhesion to loricrin is enhanced through force-induced conformational changes in the ClfB molecule, from a weakly binding folded state to a strongly binding extended state. This force-sensitive mechanism may provide S. aureus with a means to finely tune its adhesive properties during the colonization of host surfaces, helping cells to attach firmly under high shear stress and to detach and spread under low shear stress. |
format | Online Article Text |
id | pubmed-5717387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-57173872017-12-14 Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin Vitry, Pauline Valotteau, Claire Feuillie, Cécile Bernard, Simon Alsteens, David Geoghegan, Joan A. Dufrêne, Yves F. mBio Research Article Bacterial pathogens that colonize host surfaces are subjected to physical stresses such as fluid flow and cell surface contacts. How bacteria respond to such mechanical cues is an important yet poorly understood issue. Staphylococcus aureus uses a repertoire of surface proteins to resist shear stress during the colonization of host tissues, but whether their adhesive functions can be modulated by physical forces is not known. Here, we show that the interaction of S. aureus clumping factor B (ClfB) with the squamous epithelial cell envelope protein loricrin is enhanced by mechanical force. We find that ClfB mediates S. aureus adhesion to loricrin through weak and strong molecular interactions both in a laboratory strain and in a clinical isolate. Strong forces (~1,500 pN), among the strongest measured for a receptor-ligand bond, are consistent with a high-affinity “dock, lock, and latch” binding mechanism involving dynamic conformational changes in the adhesin. Notably, we demonstrate that the strength of the ClfB-loricrin bond increases as mechanical force is applied. These findings favor a two-state model whereby bacterial adhesion to loricrin is enhanced through force-induced conformational changes in the ClfB molecule, from a weakly binding folded state to a strongly binding extended state. This force-sensitive mechanism may provide S. aureus with a means to finely tune its adhesive properties during the colonization of host surfaces, helping cells to attach firmly under high shear stress and to detach and spread under low shear stress. American Society for Microbiology 2017-12-05 /pmc/articles/PMC5717387/ /pubmed/29208742 http://dx.doi.org/10.1128/mBio.01748-17 Text en Copyright © 2017 Vitry et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Vitry, Pauline Valotteau, Claire Feuillie, Cécile Bernard, Simon Alsteens, David Geoghegan, Joan A. Dufrêne, Yves F. Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin |
title | Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin |
title_full | Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin |
title_fullStr | Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin |
title_full_unstemmed | Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin |
title_short | Force-Induced Strengthening of the Interaction between Staphylococcus aureus Clumping Factor B and Loricrin |
title_sort | force-induced strengthening of the interaction between staphylococcus aureus clumping factor b and loricrin |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717387/ https://www.ncbi.nlm.nih.gov/pubmed/29208742 http://dx.doi.org/10.1128/mBio.01748-17 |
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