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Changes in the gut microbiota composition and the plasma ghrelin level in patients with Helicobacter pylori-infected patients with eradication therapy

OBJECTIVE: To investigate the influence of antimicrobials on both the gut microbiota structure and the plasma ghrelin level using Helicobacter pylori-infected patients who underwent eradication therapy. DESIGN: Twenty H. pylori-infected patients (mean age 68.3 years old) who underwent eradication th...

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Autores principales: Yanagi, Hidetaka, Tsuda, Ayumi, Matsushima, Masashi, Takahashi, Shunsuke, Ozawa, Genki, Koga, Yasuhiro, Takagi, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717420/
https://www.ncbi.nlm.nih.gov/pubmed/29225907
http://dx.doi.org/10.1136/bmjgast-2017-000182
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author Yanagi, Hidetaka
Tsuda, Ayumi
Matsushima, Masashi
Takahashi, Shunsuke
Ozawa, Genki
Koga, Yasuhiro
Takagi, Atsushi
author_facet Yanagi, Hidetaka
Tsuda, Ayumi
Matsushima, Masashi
Takahashi, Shunsuke
Ozawa, Genki
Koga, Yasuhiro
Takagi, Atsushi
author_sort Yanagi, Hidetaka
collection PubMed
description OBJECTIVE: To investigate the influence of antimicrobials on both the gut microbiota structure and the plasma ghrelin level using Helicobacter pylori-infected patients who underwent eradication therapy. DESIGN: Twenty H. pylori-infected patients (mean age 68.3 years old) who underwent eradication therapy participated in the study. For the therapy, patients had 1 week of triple therapy consisting of amoxicillin, clarithromycin and proton-pump inhibitors. Stool and blood samples were obtained before (S1), immediately after (S2) and/or 3 months after (S3) the therapies. The concentrations of ghrelin and leptin in the blood were assayed using an ELISA. The V3-V4 region of the 16S rRNA gene was amplified using bacterial DNA from the stool, and about 50 000 high-quality amplicons per sample were grouped into operational taxonomic units for bacteriological analyses. RESULTS: The Bacteroidetes:Firmicutes (B:F) ratio was significantly greater at S3 than S1 (P<0.01). This increase in the B:F ratio between S3 and S1 was found in 15 out of 20 patients. A significant decrease in the concentration of active ghrelin (P=0.003) in the plasma was observed between S3 and S1. There was a statistically significant correlation between the rate of patients whose B:F ratio increased and that of patients whose active ghrelin level decreased between S3 and S1 according to Fisher’s exact probability test (P=0.03). CONCLUSIONS: Changes in the gut microbiota, such as the B:F ratio after treatment with antimicrobials, might cause a change in the plasma ghrelin level, as the direct and earliest target of antimicrobials would be the microbiota rather than the hormone-secreting system.
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spelling pubmed-57174202017-12-08 Changes in the gut microbiota composition and the plasma ghrelin level in patients with Helicobacter pylori-infected patients with eradication therapy Yanagi, Hidetaka Tsuda, Ayumi Matsushima, Masashi Takahashi, Shunsuke Ozawa, Genki Koga, Yasuhiro Takagi, Atsushi BMJ Open Gastroenterol Gut Microbiota OBJECTIVE: To investigate the influence of antimicrobials on both the gut microbiota structure and the plasma ghrelin level using Helicobacter pylori-infected patients who underwent eradication therapy. DESIGN: Twenty H. pylori-infected patients (mean age 68.3 years old) who underwent eradication therapy participated in the study. For the therapy, patients had 1 week of triple therapy consisting of amoxicillin, clarithromycin and proton-pump inhibitors. Stool and blood samples were obtained before (S1), immediately after (S2) and/or 3 months after (S3) the therapies. The concentrations of ghrelin and leptin in the blood were assayed using an ELISA. The V3-V4 region of the 16S rRNA gene was amplified using bacterial DNA from the stool, and about 50 000 high-quality amplicons per sample were grouped into operational taxonomic units for bacteriological analyses. RESULTS: The Bacteroidetes:Firmicutes (B:F) ratio was significantly greater at S3 than S1 (P<0.01). This increase in the B:F ratio between S3 and S1 was found in 15 out of 20 patients. A significant decrease in the concentration of active ghrelin (P=0.003) in the plasma was observed between S3 and S1. There was a statistically significant correlation between the rate of patients whose B:F ratio increased and that of patients whose active ghrelin level decreased between S3 and S1 according to Fisher’s exact probability test (P=0.03). CONCLUSIONS: Changes in the gut microbiota, such as the B:F ratio after treatment with antimicrobials, might cause a change in the plasma ghrelin level, as the direct and earliest target of antimicrobials would be the microbiota rather than the hormone-secreting system. BMJ Publishing Group 2017-11-26 /pmc/articles/PMC5717420/ /pubmed/29225907 http://dx.doi.org/10.1136/bmjgast-2017-000182 Text en © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted. This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Gut Microbiota
Yanagi, Hidetaka
Tsuda, Ayumi
Matsushima, Masashi
Takahashi, Shunsuke
Ozawa, Genki
Koga, Yasuhiro
Takagi, Atsushi
Changes in the gut microbiota composition and the plasma ghrelin level in patients with Helicobacter pylori-infected patients with eradication therapy
title Changes in the gut microbiota composition and the plasma ghrelin level in patients with Helicobacter pylori-infected patients with eradication therapy
title_full Changes in the gut microbiota composition and the plasma ghrelin level in patients with Helicobacter pylori-infected patients with eradication therapy
title_fullStr Changes in the gut microbiota composition and the plasma ghrelin level in patients with Helicobacter pylori-infected patients with eradication therapy
title_full_unstemmed Changes in the gut microbiota composition and the plasma ghrelin level in patients with Helicobacter pylori-infected patients with eradication therapy
title_short Changes in the gut microbiota composition and the plasma ghrelin level in patients with Helicobacter pylori-infected patients with eradication therapy
title_sort changes in the gut microbiota composition and the plasma ghrelin level in patients with helicobacter pylori-infected patients with eradication therapy
topic Gut Microbiota
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717420/
https://www.ncbi.nlm.nih.gov/pubmed/29225907
http://dx.doi.org/10.1136/bmjgast-2017-000182
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