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Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract

Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) b...

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Autores principales: Nursyirwani, Nursyirwani, Asmara, Widya, Wahyuni, Agnesia Endang Tri Hastuti, Triyanto, Triyanto, Fauzi, Muhammad, Muchlisin, Zainal Abidin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717476/
https://www.ncbi.nlm.nih.gov/pubmed/29259771
http://dx.doi.org/10.12688/f1000research.12734.1
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author Nursyirwani, Nursyirwani
Asmara, Widya
Wahyuni, Agnesia Endang Tri Hastuti
Triyanto, Triyanto
Fauzi, Muhammad
Muchlisin, Zainal Abidin
author_facet Nursyirwani, Nursyirwani
Asmara, Widya
Wahyuni, Agnesia Endang Tri Hastuti
Triyanto, Triyanto
Fauzi, Muhammad
Muchlisin, Zainal Abidin
author_sort Nursyirwani, Nursyirwani
collection PubMed
description Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the  temperatures of 10 (o)C and 45 (o)C, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following  the Standard Analytical Profile Index, API 50 CH kit.  The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus Enterococcus. 16S rDNA sequence analysis indicated that the isolates had 99% similarity to E. hirae ATCC 9790, registered in GenBank with accession number NR_075022.1. It was concluded that the three LAB isolates taken from the tiger grouper Epinephelus fuscoguttatus are E. hirae.
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spelling pubmed-57174762017-12-18 Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract Nursyirwani, Nursyirwani Asmara, Widya Wahyuni, Agnesia Endang Tri Hastuti Triyanto, Triyanto Fauzi, Muhammad Muchlisin, Zainal Abidin F1000Res Research Note Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the  temperatures of 10 (o)C and 45 (o)C, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following  the Standard Analytical Profile Index, API 50 CH kit.  The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus Enterococcus. 16S rDNA sequence analysis indicated that the isolates had 99% similarity to E. hirae ATCC 9790, registered in GenBank with accession number NR_075022.1. It was concluded that the three LAB isolates taken from the tiger grouper Epinephelus fuscoguttatus are E. hirae. F1000 Research Limited 2017-11-10 /pmc/articles/PMC5717476/ /pubmed/29259771 http://dx.doi.org/10.12688/f1000research.12734.1 Text en Copyright: © 2017 Nursyirwani N et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Note
Nursyirwani, Nursyirwani
Asmara, Widya
Wahyuni, Agnesia Endang Tri Hastuti
Triyanto, Triyanto
Fauzi, Muhammad
Muchlisin, Zainal Abidin
Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract
title Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract
title_full Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract
title_fullStr Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract
title_full_unstemmed Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract
title_short Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract
title_sort phenotype and genotype of  lactic acid bacteria (lab) isolated from the tiger grouper epinephelus fuscoguttatus alimentary tract
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717476/
https://www.ncbi.nlm.nih.gov/pubmed/29259771
http://dx.doi.org/10.12688/f1000research.12734.1
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