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Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract
Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) b...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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F1000 Research Limited
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717476/ https://www.ncbi.nlm.nih.gov/pubmed/29259771 http://dx.doi.org/10.12688/f1000research.12734.1 |
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author | Nursyirwani, Nursyirwani Asmara, Widya Wahyuni, Agnesia Endang Tri Hastuti Triyanto, Triyanto Fauzi, Muhammad Muchlisin, Zainal Abidin |
author_facet | Nursyirwani, Nursyirwani Asmara, Widya Wahyuni, Agnesia Endang Tri Hastuti Triyanto, Triyanto Fauzi, Muhammad Muchlisin, Zainal Abidin |
author_sort | Nursyirwani, Nursyirwani |
collection | PubMed |
description | Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the temperatures of 10 (o)C and 45 (o)C, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following the Standard Analytical Profile Index, API 50 CH kit. The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus Enterococcus. 16S rDNA sequence analysis indicated that the isolates had 99% similarity to E. hirae ATCC 9790, registered in GenBank with accession number NR_075022.1. It was concluded that the three LAB isolates taken from the tiger grouper Epinephelus fuscoguttatus are E. hirae. |
format | Online Article Text |
id | pubmed-5717476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-57174762017-12-18 Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract Nursyirwani, Nursyirwani Asmara, Widya Wahyuni, Agnesia Endang Tri Hastuti Triyanto, Triyanto Fauzi, Muhammad Muchlisin, Zainal Abidin F1000Res Research Note Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper Epinephelus fuscoguttatus intestine. However, their genus or species have not been identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the temperatures of 10 (o)C and 45 (o)C, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following the Standard Analytical Profile Index, API 50 CH kit. The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus Enterococcus. 16S rDNA sequence analysis indicated that the isolates had 99% similarity to E. hirae ATCC 9790, registered in GenBank with accession number NR_075022.1. It was concluded that the three LAB isolates taken from the tiger grouper Epinephelus fuscoguttatus are E. hirae. F1000 Research Limited 2017-11-10 /pmc/articles/PMC5717476/ /pubmed/29259771 http://dx.doi.org/10.12688/f1000research.12734.1 Text en Copyright: © 2017 Nursyirwani N et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Note Nursyirwani, Nursyirwani Asmara, Widya Wahyuni, Agnesia Endang Tri Hastuti Triyanto, Triyanto Fauzi, Muhammad Muchlisin, Zainal Abidin Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper Epinephelus fuscoguttatus alimentary tract |
title | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper
Epinephelus fuscoguttatus alimentary tract |
title_full | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper
Epinephelus fuscoguttatus alimentary tract |
title_fullStr | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper
Epinephelus fuscoguttatus alimentary tract |
title_full_unstemmed | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper
Epinephelus fuscoguttatus alimentary tract |
title_short | Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper
Epinephelus fuscoguttatus alimentary tract |
title_sort | phenotype and genotype of lactic acid bacteria (lab) isolated from the tiger grouper
epinephelus fuscoguttatus alimentary tract |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717476/ https://www.ncbi.nlm.nih.gov/pubmed/29259771 http://dx.doi.org/10.12688/f1000research.12734.1 |
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