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Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks
BACKGROUND: Campylobacter jejuni is a major cause of foodborne disease having chickens as an important reservoir. Its control at the farm would lower the contamination of the final products and therefore also lower the risk of transmission to humans. At the farm, C. jejuni is rarely found in chicken...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717825/ https://www.ncbi.nlm.nih.gov/pubmed/29208016 http://dx.doi.org/10.1186/s13028-017-0346-4 |
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author | Thibodeau, Alexandre Fravalo, Philippe Perron, Audrey Lewandowski, Sylvette Laurent- Letellier, Ann |
author_facet | Thibodeau, Alexandre Fravalo, Philippe Perron, Audrey Lewandowski, Sylvette Laurent- Letellier, Ann |
author_sort | Thibodeau, Alexandre |
collection | PubMed |
description | BACKGROUND: Campylobacter jejuni is a major cause of foodborne disease having chickens as an important reservoir. Its control at the farm would lower the contamination of the final products and therefore also lower the risk of transmission to humans. At the farm, C. jejuni is rarely found in chickens before they reach 2 weeks of age. Past studies have shown that maternal antibodies could hamper C. jejuni gut colonization. The objective of this study was to compare protocols to use in order to produce anti-C. jejuni antibodies derived from egg yolks in the perspective to be used as feed additives for the control of chicken C. jejuni colonization. Laying hens were naturally contaminated with four well-characterized strains or injected with either outer membrane proteins or formalin-killed whole bacteria derived from these same strains. Eggs were collected and IgYs present in the yolks were extracted. The amount and the specificity of the recovered antibodies were characterized. RESULTS: It was observed that injection yielded eggs with superior concentrations of both total and anti-C. jejuni antibodies. Equivalent performances for antibodies recovered from all protocols were observed for the ability of the antibodies to agglutinate the live C. jejuni homologous strains, to hinder their motility or to lyse the bacteria. Western blot analyses showed that proteins from all strains could be recognized by all IgY extracts. All these characteristics were strain specific. The characterization assays were also made for heterologous strains and weaker results were observed when compared to the homologous strains. CONCLUSIONS: Based on these results, only an IgY quantitative based selection can be made in regards to which protocol would give the best anti-C. jejuni IgY enriched egg-yolks as all tested protocols were equivalent in terms of the recovered antibody ability to recognized the tested C. jejuni strains. |
format | Online Article Text |
id | pubmed-5717825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57178252017-12-08 Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks Thibodeau, Alexandre Fravalo, Philippe Perron, Audrey Lewandowski, Sylvette Laurent- Letellier, Ann Acta Vet Scand Research BACKGROUND: Campylobacter jejuni is a major cause of foodborne disease having chickens as an important reservoir. Its control at the farm would lower the contamination of the final products and therefore also lower the risk of transmission to humans. At the farm, C. jejuni is rarely found in chickens before they reach 2 weeks of age. Past studies have shown that maternal antibodies could hamper C. jejuni gut colonization. The objective of this study was to compare protocols to use in order to produce anti-C. jejuni antibodies derived from egg yolks in the perspective to be used as feed additives for the control of chicken C. jejuni colonization. Laying hens were naturally contaminated with four well-characterized strains or injected with either outer membrane proteins or formalin-killed whole bacteria derived from these same strains. Eggs were collected and IgYs present in the yolks were extracted. The amount and the specificity of the recovered antibodies were characterized. RESULTS: It was observed that injection yielded eggs with superior concentrations of both total and anti-C. jejuni antibodies. Equivalent performances for antibodies recovered from all protocols were observed for the ability of the antibodies to agglutinate the live C. jejuni homologous strains, to hinder their motility or to lyse the bacteria. Western blot analyses showed that proteins from all strains could be recognized by all IgY extracts. All these characteristics were strain specific. The characterization assays were also made for heterologous strains and weaker results were observed when compared to the homologous strains. CONCLUSIONS: Based on these results, only an IgY quantitative based selection can be made in regards to which protocol would give the best anti-C. jejuni IgY enriched egg-yolks as all tested protocols were equivalent in terms of the recovered antibody ability to recognized the tested C. jejuni strains. BioMed Central 2017-12-06 /pmc/articles/PMC5717825/ /pubmed/29208016 http://dx.doi.org/10.1186/s13028-017-0346-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Thibodeau, Alexandre Fravalo, Philippe Perron, Audrey Lewandowski, Sylvette Laurent- Letellier, Ann Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks |
title | Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks |
title_full | Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks |
title_fullStr | Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks |
title_full_unstemmed | Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks |
title_short | Production and characterization of anti-Campylobacter jejuni IgY derived from egg yolks |
title_sort | production and characterization of anti-campylobacter jejuni igy derived from egg yolks |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717825/ https://www.ncbi.nlm.nih.gov/pubmed/29208016 http://dx.doi.org/10.1186/s13028-017-0346-4 |
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