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Babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations
BACKGROUND: Babesia spp. are hemoparasites which infect the red blood cells of a large variety of mammals. In bats, the only known species of the genus is Babesia vesperuginis. However, except a few old reports, the host range and geographical distribution of this bat parasite have been poorly studi...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718032/ https://www.ncbi.nlm.nih.gov/pubmed/29208011 http://dx.doi.org/10.1186/s13071-017-2536-3 |
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author | Corduneanu, Alexandra Hrazdilová, Kristýna Sándor, Attila D. Matei, Ioana Adriana Ionică, Angela Monica Barti, Levente Ciocănău, Marius-Alexandru Măntoiu, Dragoş Ștefan Coroiu, Ioan Hornok, Sándor Fuehrer, Hans-Peter Leitner, Natascha Bagó, Zoltán Stefke, Katharina Modrý, David Mihalca, Andrei Daniel |
author_facet | Corduneanu, Alexandra Hrazdilová, Kristýna Sándor, Attila D. Matei, Ioana Adriana Ionică, Angela Monica Barti, Levente Ciocănău, Marius-Alexandru Măntoiu, Dragoş Ștefan Coroiu, Ioan Hornok, Sándor Fuehrer, Hans-Peter Leitner, Natascha Bagó, Zoltán Stefke, Katharina Modrý, David Mihalca, Andrei Daniel |
author_sort | Corduneanu, Alexandra |
collection | PubMed |
description | BACKGROUND: Babesia spp. are hemoparasites which infect the red blood cells of a large variety of mammals. In bats, the only known species of the genus is Babesia vesperuginis. However, except a few old reports, the host range and geographical distribution of this bat parasite have been poorly studied. This study aimed to investigate the presence of piroplasms in tissues of bats collected in four different countries from eastern and central Europe: Austria, Czech Republic, Hungary and Romania. METHODS: A total of 461 bat carcasses (24 species) were collected between 2001 and 2016 from caves, mines and buildings. PCR was performed using specific primers targeting a portion of the 18S rDNA nuclear gene and cytochrome c oxidase subunit 1 mitochondrial gene, followed by sequencing. RESULTS: The results of this study show for the first time the presence of B. vesperuginis in bats in central and eastern Europe. The phylogenetic analysis of the 18S rDNA nuclear gene revealed no variability between the sequences and the phylogenetic analysis of the cox1 mitochondrial gene proved that B. vesperuginis could be divided into two subclades. CONCLUSION: Our study showed a broad geographical distribution of B. vesperuginis in European bats, reporting its presence in five new host species (M. cf. alcathoe, M. bechsteinii, M. myotis, Pi. nathusii and V. murinus) and three new countries. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-017-2536-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5718032 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57180322017-12-08 Babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations Corduneanu, Alexandra Hrazdilová, Kristýna Sándor, Attila D. Matei, Ioana Adriana Ionică, Angela Monica Barti, Levente Ciocănău, Marius-Alexandru Măntoiu, Dragoş Ștefan Coroiu, Ioan Hornok, Sándor Fuehrer, Hans-Peter Leitner, Natascha Bagó, Zoltán Stefke, Katharina Modrý, David Mihalca, Andrei Daniel Parasit Vectors Research BACKGROUND: Babesia spp. are hemoparasites which infect the red blood cells of a large variety of mammals. In bats, the only known species of the genus is Babesia vesperuginis. However, except a few old reports, the host range and geographical distribution of this bat parasite have been poorly studied. This study aimed to investigate the presence of piroplasms in tissues of bats collected in four different countries from eastern and central Europe: Austria, Czech Republic, Hungary and Romania. METHODS: A total of 461 bat carcasses (24 species) were collected between 2001 and 2016 from caves, mines and buildings. PCR was performed using specific primers targeting a portion of the 18S rDNA nuclear gene and cytochrome c oxidase subunit 1 mitochondrial gene, followed by sequencing. RESULTS: The results of this study show for the first time the presence of B. vesperuginis in bats in central and eastern Europe. The phylogenetic analysis of the 18S rDNA nuclear gene revealed no variability between the sequences and the phylogenetic analysis of the cox1 mitochondrial gene proved that B. vesperuginis could be divided into two subclades. CONCLUSION: Our study showed a broad geographical distribution of B. vesperuginis in European bats, reporting its presence in five new host species (M. cf. alcathoe, M. bechsteinii, M. myotis, Pi. nathusii and V. murinus) and three new countries. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-017-2536-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-06 /pmc/articles/PMC5718032/ /pubmed/29208011 http://dx.doi.org/10.1186/s13071-017-2536-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Corduneanu, Alexandra Hrazdilová, Kristýna Sándor, Attila D. Matei, Ioana Adriana Ionică, Angela Monica Barti, Levente Ciocănău, Marius-Alexandru Măntoiu, Dragoş Ștefan Coroiu, Ioan Hornok, Sándor Fuehrer, Hans-Peter Leitner, Natascha Bagó, Zoltán Stefke, Katharina Modrý, David Mihalca, Andrei Daniel Babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations |
title | Babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations |
title_full | Babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations |
title_fullStr | Babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations |
title_full_unstemmed | Babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations |
title_short | Babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations |
title_sort | babesia vesperuginis, a neglected piroplasmid: new host and geographical records, and phylogenetic relations |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718032/ https://www.ncbi.nlm.nih.gov/pubmed/29208011 http://dx.doi.org/10.1186/s13071-017-2536-3 |
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