Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression

CRISPR/Cas9 technology enables efficient, rapid and cost-effective targeted genomic modification in a wide variety of cellular contexts including cultured cells. Some applications such as generation of double knock-outs, large deletions and paired-nickase cleavage require simultaneous expression of...

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Detalles Bibliográficos
Autores principales: Adikusuma, Fatwa, Pfitzner, Chandran, Thomas, Paul Quinton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718404/
https://www.ncbi.nlm.nih.gov/pubmed/29211736
http://dx.doi.org/10.1371/journal.pone.0187236

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718404/
https://www.ncbi.nlm.nih.gov/pubmed/29211736
http://dx.doi.org/10.1371/journal.pone.0187236

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