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Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression
CRISPR/Cas9 technology enables efficient, rapid and cost-effective targeted genomic modification in a wide variety of cellular contexts including cultured cells. Some applications such as generation of double knock-outs, large deletions and paired-nickase cleavage require simultaneous expression of...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718404/ https://www.ncbi.nlm.nih.gov/pubmed/29211736 http://dx.doi.org/10.1371/journal.pone.0187236 |