Cargando…
Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh
BACKGROUND: Arsenic has antimicrobial properties at high doses yet few studies have examined its effect on gut microbiota. This warrants investigation since arsenic exposure increases the risk of many diseases in which gut microbiota have been shown to play a role. We examined the association betwee...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718612/ https://www.ncbi.nlm.nih.gov/pubmed/29211769 http://dx.doi.org/10.1371/journal.pone.0188487 |
_version_ | 1783284350663524352 |
---|---|
author | Dong, Xiaoxi Shulzhenko, Natalia Lemaitre, Julien Greer, Renee L. Peremyslova, Kate Quamruzzaman, Quazi Rahman, Mahmudar Hasan, Omar Sharif Ibn Joya, Sakila Afroz Golam, Mostofa Christiani, David C. Morgun, Andriy Kile, Molly L. |
author_facet | Dong, Xiaoxi Shulzhenko, Natalia Lemaitre, Julien Greer, Renee L. Peremyslova, Kate Quamruzzaman, Quazi Rahman, Mahmudar Hasan, Omar Sharif Ibn Joya, Sakila Afroz Golam, Mostofa Christiani, David C. Morgun, Andriy Kile, Molly L. |
author_sort | Dong, Xiaoxi |
collection | PubMed |
description | BACKGROUND: Arsenic has antimicrobial properties at high doses yet few studies have examined its effect on gut microbiota. This warrants investigation since arsenic exposure increases the risk of many diseases in which gut microbiota have been shown to play a role. We examined the association between arsenic exposure from drinking water and the composition of intestinal microbiota in children exposed to low and high arsenic levels during prenatal development and early life. RESULTS: 16S rRNA gene sequencing revealed that children with high arsenic exposure had a higher abundance of Proteobacteria in their stool compared to matched controls with low arsenic exposure. Furthermore, whole metagenome shotgun sequencing identified 332 bacterial SEED functions that were enriched in the high exposure group. A separate model showed that these genes, which included genes involved in virulence and multidrug resistance, were positively correlated with arsenic concentration within the group of children in the high arsenic group. We performed reference free genome assembly, and identified strains of E.coli as contributors to the arsenic enriched SEED functions. Further genome annotation of the E.coli genome revealed two strains containing two different arsenic resistance operons that are not present in the gut microbiome of a recently described European human cohort (Metagenomics of the Human Intestinal Tract, MetaHIT). We then performed quantification by qPCR of two arsenic resistant genes (ArsB, ArsC). We observed that the expression of these two operons was higher among the children with high arsenic exposure compared to matched controls. CONCLUSIONS: This preliminary study indicates that arsenic exposure early in life was associated with altered gut microbiota in Bangladeshi children. The enrichment of E.coli arsenic resistance genes in the high exposure group provides an insight into the possible mechanisms of how this toxic compound could affect gut microbiota. |
format | Online Article Text |
id | pubmed-5718612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57186122017-12-15 Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh Dong, Xiaoxi Shulzhenko, Natalia Lemaitre, Julien Greer, Renee L. Peremyslova, Kate Quamruzzaman, Quazi Rahman, Mahmudar Hasan, Omar Sharif Ibn Joya, Sakila Afroz Golam, Mostofa Christiani, David C. Morgun, Andriy Kile, Molly L. PLoS One Research Article BACKGROUND: Arsenic has antimicrobial properties at high doses yet few studies have examined its effect on gut microbiota. This warrants investigation since arsenic exposure increases the risk of many diseases in which gut microbiota have been shown to play a role. We examined the association between arsenic exposure from drinking water and the composition of intestinal microbiota in children exposed to low and high arsenic levels during prenatal development and early life. RESULTS: 16S rRNA gene sequencing revealed that children with high arsenic exposure had a higher abundance of Proteobacteria in their stool compared to matched controls with low arsenic exposure. Furthermore, whole metagenome shotgun sequencing identified 332 bacterial SEED functions that were enriched in the high exposure group. A separate model showed that these genes, which included genes involved in virulence and multidrug resistance, were positively correlated with arsenic concentration within the group of children in the high arsenic group. We performed reference free genome assembly, and identified strains of E.coli as contributors to the arsenic enriched SEED functions. Further genome annotation of the E.coli genome revealed two strains containing two different arsenic resistance operons that are not present in the gut microbiome of a recently described European human cohort (Metagenomics of the Human Intestinal Tract, MetaHIT). We then performed quantification by qPCR of two arsenic resistant genes (ArsB, ArsC). We observed that the expression of these two operons was higher among the children with high arsenic exposure compared to matched controls. CONCLUSIONS: This preliminary study indicates that arsenic exposure early in life was associated with altered gut microbiota in Bangladeshi children. The enrichment of E.coli arsenic resistance genes in the high exposure group provides an insight into the possible mechanisms of how this toxic compound could affect gut microbiota. Public Library of Science 2017-12-06 /pmc/articles/PMC5718612/ /pubmed/29211769 http://dx.doi.org/10.1371/journal.pone.0188487 Text en © 2017 Dong et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dong, Xiaoxi Shulzhenko, Natalia Lemaitre, Julien Greer, Renee L. Peremyslova, Kate Quamruzzaman, Quazi Rahman, Mahmudar Hasan, Omar Sharif Ibn Joya, Sakila Afroz Golam, Mostofa Christiani, David C. Morgun, Andriy Kile, Molly L. Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh |
title | Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh |
title_full | Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh |
title_fullStr | Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh |
title_full_unstemmed | Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh |
title_short | Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh |
title_sort | arsenic exposure and intestinal microbiota in children from sirajdikhan, bangladesh |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718612/ https://www.ncbi.nlm.nih.gov/pubmed/29211769 http://dx.doi.org/10.1371/journal.pone.0188487 |
work_keys_str_mv | AT dongxiaoxi arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT shulzhenkonatalia arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT lemaitrejulien arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT greerreneel arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT peremyslovakate arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT quamruzzamanquazi arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT rahmanmahmudar arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT hasanomarsharifibn arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT joyasakilaafroz arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT golammostofa arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT christianidavidc arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT morgunandriy arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh AT kilemollyl arsenicexposureandintestinalmicrobiotainchildrenfromsirajdikhanbangladesh |