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Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh

BACKGROUND: Arsenic has antimicrobial properties at high doses yet few studies have examined its effect on gut microbiota. This warrants investigation since arsenic exposure increases the risk of many diseases in which gut microbiota have been shown to play a role. We examined the association betwee...

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Autores principales: Dong, Xiaoxi, Shulzhenko, Natalia, Lemaitre, Julien, Greer, Renee L., Peremyslova, Kate, Quamruzzaman, Quazi, Rahman, Mahmudar, Hasan, Omar Sharif Ibn, Joya, Sakila Afroz, Golam, Mostofa, Christiani, David C., Morgun, Andriy, Kile, Molly L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718612/
https://www.ncbi.nlm.nih.gov/pubmed/29211769
http://dx.doi.org/10.1371/journal.pone.0188487
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author Dong, Xiaoxi
Shulzhenko, Natalia
Lemaitre, Julien
Greer, Renee L.
Peremyslova, Kate
Quamruzzaman, Quazi
Rahman, Mahmudar
Hasan, Omar Sharif Ibn
Joya, Sakila Afroz
Golam, Mostofa
Christiani, David C.
Morgun, Andriy
Kile, Molly L.
author_facet Dong, Xiaoxi
Shulzhenko, Natalia
Lemaitre, Julien
Greer, Renee L.
Peremyslova, Kate
Quamruzzaman, Quazi
Rahman, Mahmudar
Hasan, Omar Sharif Ibn
Joya, Sakila Afroz
Golam, Mostofa
Christiani, David C.
Morgun, Andriy
Kile, Molly L.
author_sort Dong, Xiaoxi
collection PubMed
description BACKGROUND: Arsenic has antimicrobial properties at high doses yet few studies have examined its effect on gut microbiota. This warrants investigation since arsenic exposure increases the risk of many diseases in which gut microbiota have been shown to play a role. We examined the association between arsenic exposure from drinking water and the composition of intestinal microbiota in children exposed to low and high arsenic levels during prenatal development and early life. RESULTS: 16S rRNA gene sequencing revealed that children with high arsenic exposure had a higher abundance of Proteobacteria in their stool compared to matched controls with low arsenic exposure. Furthermore, whole metagenome shotgun sequencing identified 332 bacterial SEED functions that were enriched in the high exposure group. A separate model showed that these genes, which included genes involved in virulence and multidrug resistance, were positively correlated with arsenic concentration within the group of children in the high arsenic group. We performed reference free genome assembly, and identified strains of E.coli as contributors to the arsenic enriched SEED functions. Further genome annotation of the E.coli genome revealed two strains containing two different arsenic resistance operons that are not present in the gut microbiome of a recently described European human cohort (Metagenomics of the Human Intestinal Tract, MetaHIT). We then performed quantification by qPCR of two arsenic resistant genes (ArsB, ArsC). We observed that the expression of these two operons was higher among the children with high arsenic exposure compared to matched controls. CONCLUSIONS: This preliminary study indicates that arsenic exposure early in life was associated with altered gut microbiota in Bangladeshi children. The enrichment of E.coli arsenic resistance genes in the high exposure group provides an insight into the possible mechanisms of how this toxic compound could affect gut microbiota.
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spelling pubmed-57186122017-12-15 Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh Dong, Xiaoxi Shulzhenko, Natalia Lemaitre, Julien Greer, Renee L. Peremyslova, Kate Quamruzzaman, Quazi Rahman, Mahmudar Hasan, Omar Sharif Ibn Joya, Sakila Afroz Golam, Mostofa Christiani, David C. Morgun, Andriy Kile, Molly L. PLoS One Research Article BACKGROUND: Arsenic has antimicrobial properties at high doses yet few studies have examined its effect on gut microbiota. This warrants investigation since arsenic exposure increases the risk of many diseases in which gut microbiota have been shown to play a role. We examined the association between arsenic exposure from drinking water and the composition of intestinal microbiota in children exposed to low and high arsenic levels during prenatal development and early life. RESULTS: 16S rRNA gene sequencing revealed that children with high arsenic exposure had a higher abundance of Proteobacteria in their stool compared to matched controls with low arsenic exposure. Furthermore, whole metagenome shotgun sequencing identified 332 bacterial SEED functions that were enriched in the high exposure group. A separate model showed that these genes, which included genes involved in virulence and multidrug resistance, were positively correlated with arsenic concentration within the group of children in the high arsenic group. We performed reference free genome assembly, and identified strains of E.coli as contributors to the arsenic enriched SEED functions. Further genome annotation of the E.coli genome revealed two strains containing two different arsenic resistance operons that are not present in the gut microbiome of a recently described European human cohort (Metagenomics of the Human Intestinal Tract, MetaHIT). We then performed quantification by qPCR of two arsenic resistant genes (ArsB, ArsC). We observed that the expression of these two operons was higher among the children with high arsenic exposure compared to matched controls. CONCLUSIONS: This preliminary study indicates that arsenic exposure early in life was associated with altered gut microbiota in Bangladeshi children. The enrichment of E.coli arsenic resistance genes in the high exposure group provides an insight into the possible mechanisms of how this toxic compound could affect gut microbiota. Public Library of Science 2017-12-06 /pmc/articles/PMC5718612/ /pubmed/29211769 http://dx.doi.org/10.1371/journal.pone.0188487 Text en © 2017 Dong et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dong, Xiaoxi
Shulzhenko, Natalia
Lemaitre, Julien
Greer, Renee L.
Peremyslova, Kate
Quamruzzaman, Quazi
Rahman, Mahmudar
Hasan, Omar Sharif Ibn
Joya, Sakila Afroz
Golam, Mostofa
Christiani, David C.
Morgun, Andriy
Kile, Molly L.
Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh
title Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh
title_full Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh
title_fullStr Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh
title_full_unstemmed Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh
title_short Arsenic exposure and intestinal microbiota in children from Sirajdikhan, Bangladesh
title_sort arsenic exposure and intestinal microbiota in children from sirajdikhan, bangladesh
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5718612/
https://www.ncbi.nlm.nih.gov/pubmed/29211769
http://dx.doi.org/10.1371/journal.pone.0188487
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