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Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat

Accumulating evidence demonstrates that microcirculation plays a role in the pathogenesis of hypertension. In the current study, we demonstrated that pancreatic islet microvascular vasomotion of spontaneously hypertensive rats (SHRs) lost the ability to regulate blood flow perfusion and exhibited a...

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Autores principales: Liu, Mingming, Zhang, Xiaoyan, Wang, Bing, Wu, Qingbin, Li, Bingwei, Li, Ailing, Zhang, Honggang, Xiu, Ruijuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719042/
https://www.ncbi.nlm.nih.gov/pubmed/29213078
http://dx.doi.org/10.1038/s41598-017-17013-w
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author Liu, Mingming
Zhang, Xiaoyan
Wang, Bing
Wu, Qingbin
Li, Bingwei
Li, Ailing
Zhang, Honggang
Xiu, Ruijuan
author_facet Liu, Mingming
Zhang, Xiaoyan
Wang, Bing
Wu, Qingbin
Li, Bingwei
Li, Ailing
Zhang, Honggang
Xiu, Ruijuan
author_sort Liu, Mingming
collection PubMed
description Accumulating evidence demonstrates that microcirculation plays a role in the pathogenesis of hypertension. In the current study, we demonstrated that pancreatic islet microvascular vasomotion of spontaneously hypertensive rats (SHRs) lost the ability to regulate blood flow perfusion and exhibited a lower microvascular blood perfusion pattern which was negative correlated with blood glucose level. SHRs administrated with insulin revealed an improvement of pancreatic islet microvascular vasomotion and blood perfusion pattern. In vitro, the expressions of endothelial nitric oxide synthase (eNOS) and phospho-eNOS(ser1177) (p-eNOS(ser1177)) were significantly decreased in high glucose exposed islet endothelial cells (iECs), accompanied with a higher ratio of eNOS monomer to eNOS dimer and a significantly increased malondialdehyde and nitrite levels. Meanwhile, barrier function, tube formation and migration capacities of high glucose exposed iECs were significantly inhibited. In contrast, iECs dysfunction induced by glucose toxicity and oxidative stress was attenuated or improved by supplement with insulin, L-arginine and β-mercaptoethanol. In summary, our findings suggest that functional status of pancreatic islet microvascular vasomotion is impaired in SHRs and provide evidence that treatment with insulin, L-arginine and β-mercaptoethanol improves endothelium-dependent microvascular vasomotion and meliorates iECs function due to anti-hyperglycemic and anti-oxidative effects, partly through mechanism involving regulation of eNOS and p-eNOS(ser1177).
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spelling pubmed-57190422017-12-08 Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat Liu, Mingming Zhang, Xiaoyan Wang, Bing Wu, Qingbin Li, Bingwei Li, Ailing Zhang, Honggang Xiu, Ruijuan Sci Rep Article Accumulating evidence demonstrates that microcirculation plays a role in the pathogenesis of hypertension. In the current study, we demonstrated that pancreatic islet microvascular vasomotion of spontaneously hypertensive rats (SHRs) lost the ability to regulate blood flow perfusion and exhibited a lower microvascular blood perfusion pattern which was negative correlated with blood glucose level. SHRs administrated with insulin revealed an improvement of pancreatic islet microvascular vasomotion and blood perfusion pattern. In vitro, the expressions of endothelial nitric oxide synthase (eNOS) and phospho-eNOS(ser1177) (p-eNOS(ser1177)) were significantly decreased in high glucose exposed islet endothelial cells (iECs), accompanied with a higher ratio of eNOS monomer to eNOS dimer and a significantly increased malondialdehyde and nitrite levels. Meanwhile, barrier function, tube formation and migration capacities of high glucose exposed iECs were significantly inhibited. In contrast, iECs dysfunction induced by glucose toxicity and oxidative stress was attenuated or improved by supplement with insulin, L-arginine and β-mercaptoethanol. In summary, our findings suggest that functional status of pancreatic islet microvascular vasomotion is impaired in SHRs and provide evidence that treatment with insulin, L-arginine and β-mercaptoethanol improves endothelium-dependent microvascular vasomotion and meliorates iECs function due to anti-hyperglycemic and anti-oxidative effects, partly through mechanism involving regulation of eNOS and p-eNOS(ser1177). Nature Publishing Group UK 2017-12-06 /pmc/articles/PMC5719042/ /pubmed/29213078 http://dx.doi.org/10.1038/s41598-017-17013-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Mingming
Zhang, Xiaoyan
Wang, Bing
Wu, Qingbin
Li, Bingwei
Li, Ailing
Zhang, Honggang
Xiu, Ruijuan
Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat
title Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat
title_full Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat
title_fullStr Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat
title_full_unstemmed Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat
title_short Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat
title_sort functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719042/
https://www.ncbi.nlm.nih.gov/pubmed/29213078
http://dx.doi.org/10.1038/s41598-017-17013-w
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