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Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation
Excessive long-term fluoride intake is associated with several health problems, including infertility. However, limited information is available on the toxic effects of fluoride exposure on the female reproductive system, especially oocyte maturation. In this study, we investigated the toxic effect...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719058/ https://www.ncbi.nlm.nih.gov/pubmed/29213094 http://dx.doi.org/10.1038/s41598-017-17357-3 |
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author | Liang, Shuang Nie, Zheng-Wen Zhao, Minghui Niu, Ying-Jie Shin, Kyung-Tae Cui, Xiang-Shun |
author_facet | Liang, Shuang Nie, Zheng-Wen Zhao, Minghui Niu, Ying-Jie Shin, Kyung-Tae Cui, Xiang-Shun |
author_sort | Liang, Shuang |
collection | PubMed |
description | Excessive long-term fluoride intake is associated with several health problems, including infertility. However, limited information is available on the toxic effects of fluoride exposure on the female reproductive system, especially oocyte maturation. In this study, we investigated the toxic effect of sodium fluoride (NaF) exposure on porcine oocyte maturation and its possible underlying mechanisms. Our results showed that NaF exposure during porcine oocyte maturation inhibited cumulus cell expansion and impaired polar body extrusion. Cell cycle analysis showed that NaF exposure blocked meiotic resumption, disturbed spindle dynamics, disrupted chromosome separation, and increased aneuploidy in porcine oocytes. Moreover, NaF exposure disturbed mitochondrial function, triggered DNA damage response, and induced early apoptosis in porcine oocytes. NaF exposure also induced oxidative stress, decreased GSH level, and increased cathepsin B activity in and impaired the further development potential of porcine oocytes, as indicated by a decrease in blastocyst formation rate, increase in apoptosis, and inhibition of cell proliferation. Together, these results indicate that NaF exposure impairs the maturation capacity of porcine oocytes by inhibiting cumulus cell expansion, disturbing cytoskeletal dynamics, and blocking nuclear and cytoplasmic maturation, thus decreasing the quality and affecting the subsequent embryonic development potential of porcine oocytes. |
format | Online Article Text |
id | pubmed-5719058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57190582017-12-08 Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation Liang, Shuang Nie, Zheng-Wen Zhao, Minghui Niu, Ying-Jie Shin, Kyung-Tae Cui, Xiang-Shun Sci Rep Article Excessive long-term fluoride intake is associated with several health problems, including infertility. However, limited information is available on the toxic effects of fluoride exposure on the female reproductive system, especially oocyte maturation. In this study, we investigated the toxic effect of sodium fluoride (NaF) exposure on porcine oocyte maturation and its possible underlying mechanisms. Our results showed that NaF exposure during porcine oocyte maturation inhibited cumulus cell expansion and impaired polar body extrusion. Cell cycle analysis showed that NaF exposure blocked meiotic resumption, disturbed spindle dynamics, disrupted chromosome separation, and increased aneuploidy in porcine oocytes. Moreover, NaF exposure disturbed mitochondrial function, triggered DNA damage response, and induced early apoptosis in porcine oocytes. NaF exposure also induced oxidative stress, decreased GSH level, and increased cathepsin B activity in and impaired the further development potential of porcine oocytes, as indicated by a decrease in blastocyst formation rate, increase in apoptosis, and inhibition of cell proliferation. Together, these results indicate that NaF exposure impairs the maturation capacity of porcine oocytes by inhibiting cumulus cell expansion, disturbing cytoskeletal dynamics, and blocking nuclear and cytoplasmic maturation, thus decreasing the quality and affecting the subsequent embryonic development potential of porcine oocytes. Nature Publishing Group UK 2017-12-06 /pmc/articles/PMC5719058/ /pubmed/29213094 http://dx.doi.org/10.1038/s41598-017-17357-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liang, Shuang Nie, Zheng-Wen Zhao, Minghui Niu, Ying-Jie Shin, Kyung-Tae Cui, Xiang-Shun Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation |
title | Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation |
title_full | Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation |
title_fullStr | Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation |
title_full_unstemmed | Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation |
title_short | Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation |
title_sort | sodium fluoride exposure exerts toxic effects on porcine oocyte maturation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719058/ https://www.ncbi.nlm.nih.gov/pubmed/29213094 http://dx.doi.org/10.1038/s41598-017-17357-3 |
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