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Can sexual transmission support the enzootic cycle of Trypanosoma cruzi?
BACKGROUND: Trypanosoma cruzi circulates in sylvatic habitats, mainly through blood-feeding triatomines, although other routes also contribute to its dispersion. Sexual transmission of T. cruzi is an understudied topic, especially among wild mammals. Because of the difficulties inherent to field wor...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719536/ https://www.ncbi.nlm.nih.gov/pubmed/29211102 http://dx.doi.org/10.1590/0074-02760170025 |
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author | Rios, Adriano Ribeiro, Marcelle Sousa, Alessandro Pimentel, Fernando Hagström, Luciana Andrade, Rafael Alves, Rozeneide M Rosa, Ana de Cássia Teixeira, Antônio RL Nitz, Nadjar Hecht, Mariana M |
author_facet | Rios, Adriano Ribeiro, Marcelle Sousa, Alessandro Pimentel, Fernando Hagström, Luciana Andrade, Rafael Alves, Rozeneide M Rosa, Ana de Cássia Teixeira, Antônio RL Nitz, Nadjar Hecht, Mariana M |
author_sort | Rios, Adriano |
collection | PubMed |
description | BACKGROUND: Trypanosoma cruzi circulates in sylvatic habitats, mainly through blood-feeding triatomines, although other routes also contribute to its dispersion. Sexual transmission of T. cruzi is an understudied topic, especially among wild mammals. Because of the difficulties inherent to field work, experimentally infected mice are frequently used to evaluate the transmission of T. cruzi. OBJECTIVE: This study aimed to evaluate the sexual transmission of T. cruzi in acutely infected mice. METHODS: Male and female mice in the acute phase of Chagas disease were mated with naïve partners. Then, parasitological tests, immunohistochemistry, serological assays, and polymerase chain reaction (PCR) assays were used to detect infection. FINDINGS: Parasitological analysis showed trypomastigotes in the blood of 20% of the naïve mice after mating with infected partners. Serological assays detected anti-T. cruzi antibodies in all naïve females mated with infected males and in 60% of naïve males mated with infected females. PCR showed T. cruzi nDNA bands for all naïve mice mated with infected partners. The possibility of sexual transmission was also confirmed by visualisation of amastigotes in the testes. MAIN CONCLUSIONS: Our results demonstrate that sexual transmission of T. cruzi is an ordinary event that may contribute to maintenance of the parasite's enzootic cycle. |
format | Online Article Text |
id | pubmed-5719536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-57195362018-01-01 Can sexual transmission support the enzootic cycle of Trypanosoma cruzi? Rios, Adriano Ribeiro, Marcelle Sousa, Alessandro Pimentel, Fernando Hagström, Luciana Andrade, Rafael Alves, Rozeneide M Rosa, Ana de Cássia Teixeira, Antônio RL Nitz, Nadjar Hecht, Mariana M Mem Inst Oswaldo Cruz Article BACKGROUND: Trypanosoma cruzi circulates in sylvatic habitats, mainly through blood-feeding triatomines, although other routes also contribute to its dispersion. Sexual transmission of T. cruzi is an understudied topic, especially among wild mammals. Because of the difficulties inherent to field work, experimentally infected mice are frequently used to evaluate the transmission of T. cruzi. OBJECTIVE: This study aimed to evaluate the sexual transmission of T. cruzi in acutely infected mice. METHODS: Male and female mice in the acute phase of Chagas disease were mated with naïve partners. Then, parasitological tests, immunohistochemistry, serological assays, and polymerase chain reaction (PCR) assays were used to detect infection. FINDINGS: Parasitological analysis showed trypomastigotes in the blood of 20% of the naïve mice after mating with infected partners. Serological assays detected anti-T. cruzi antibodies in all naïve females mated with infected males and in 60% of naïve males mated with infected females. PCR showed T. cruzi nDNA bands for all naïve mice mated with infected partners. The possibility of sexual transmission was also confirmed by visualisation of amastigotes in the testes. MAIN CONCLUSIONS: Our results demonstrate that sexual transmission of T. cruzi is an ordinary event that may contribute to maintenance of the parasite's enzootic cycle. Instituto Oswaldo Cruz, Ministério da Saúde 2018-01 /pmc/articles/PMC5719536/ /pubmed/29211102 http://dx.doi.org/10.1590/0074-02760170025 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Rios, Adriano Ribeiro, Marcelle Sousa, Alessandro Pimentel, Fernando Hagström, Luciana Andrade, Rafael Alves, Rozeneide M Rosa, Ana de Cássia Teixeira, Antônio RL Nitz, Nadjar Hecht, Mariana M Can sexual transmission support the enzootic cycle of Trypanosoma cruzi? |
title | Can sexual transmission support the enzootic cycle of Trypanosoma cruzi? |
title_full | Can sexual transmission support the enzootic cycle of Trypanosoma cruzi? |
title_fullStr | Can sexual transmission support the enzootic cycle of Trypanosoma cruzi? |
title_full_unstemmed | Can sexual transmission support the enzootic cycle of Trypanosoma cruzi? |
title_short | Can sexual transmission support the enzootic cycle of Trypanosoma cruzi? |
title_sort | can sexual transmission support the enzootic cycle of trypanosoma cruzi? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719536/ https://www.ncbi.nlm.nih.gov/pubmed/29211102 http://dx.doi.org/10.1590/0074-02760170025 |
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