Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes

Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure targeting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent...

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Detalles Bibliográficos
Autores principales: Xie, Tao-Rong, Liu, Chun-Feng, Kang, Jian-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719795/
https://www.ncbi.nlm.nih.gov/pubmed/29238745
http://dx.doi.org/10.1007/s41048-017-0039-6
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author Xie, Tao-Rong
Liu, Chun-Feng
Kang, Jian-Sheng
author_facet Xie, Tao-Rong
Liu, Chun-Feng
Kang, Jian-Sheng
author_sort Xie, Tao-Rong
collection PubMed
description Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure targeting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms. Using this approach, we are able to demonstrate that the efficacy of norepinephrine-induced thermogenesis is low, and measure the maximum transient rate of temperature increase in brown adipocytes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s41048-017-0039-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-57197952017-12-11 Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes Xie, Tao-Rong Liu, Chun-Feng Kang, Jian-Sheng Biophys Rep Method Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure targeting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms. Using this approach, we are able to demonstrate that the efficacy of norepinephrine-induced thermogenesis is low, and measure the maximum transient rate of temperature increase in brown adipocytes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s41048-017-0039-6) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-05-13 2017 /pmc/articles/PMC5719795/ /pubmed/29238745 http://dx.doi.org/10.1007/s41048-017-0039-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Method
Xie, Tao-Rong
Liu, Chun-Feng
Kang, Jian-Sheng
Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes
title Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes
title_full Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes
title_fullStr Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes
title_full_unstemmed Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes
title_short Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes
title_sort dye-based mito-thermometry and its application in thermogenesis of brown adipocytes
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719795/
https://www.ncbi.nlm.nih.gov/pubmed/29238745
http://dx.doi.org/10.1007/s41048-017-0039-6
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AT kangjiansheng dyebasedmitothermometryanditsapplicationinthermogenesisofbrownadipocytes

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