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Determining the target protein localization in 3D using the combination of FIB-SEM and APEX2

Determining the cellular localization of proteins of interest at nanometer resolution is necessary for elucidating their functions. Besides super-resolution fluorescence microscopy, conventional electron microscopy (EM) combined with immunolabeling or clonable EM tags provides a unique approach to c...

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Detalles Bibliográficos
Autores principales:	Shi, Yang, Wang, Li, Zhang, Jianguo, Zhai, Yujia, Sun, Fei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2017
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719812/ https://www.ncbi.nlm.nih.gov/pubmed/29238746 http://dx.doi.org/10.1007/s41048-017-0043-x

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