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Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis
High-mobility group box-1 (HMGB-1) is expressed in almost all cells, and its dysregulated expression correlates with inflammatory diseases, ischemia, and cancer. Some of these conditions accompany HMGB-1-mediated abnormal angiogenesis. Thus far, the mechanism of HMGB-1-induced angiogenesis remains l...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Biochemistry and Molecular Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720474/ https://www.ncbi.nlm.nih.gov/pubmed/29065965 http://dx.doi.org/10.5483/BMBRep.2017.50.11.129 |
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author | Kim, Won Kyu Kwon, Yujin Park, Minhee Yun, Seongju Kwon, Ja-Young Kim, Hoguen |
author_facet | Kim, Won Kyu Kwon, Yujin Park, Minhee Yun, Seongju Kwon, Ja-Young Kim, Hoguen |
author_sort | Kim, Won Kyu |
collection | PubMed |
description | High-mobility group box-1 (HMGB-1) is expressed in almost all cells, and its dysregulated expression correlates with inflammatory diseases, ischemia, and cancer. Some of these conditions accompany HMGB-1-mediated abnormal angiogenesis. Thus far, the mechanism of HMGB-1-induced angiogenesis remains largely unknown. In this study, we performed time-dependent DNA microarray analysis of endothelial cells (ECs) after HMGB-1 or VEGF treatment. The pathway analysis of each gene set upregulated by HMGB-1 or VEGF showed that most HMGB-1-induced angiogenic pathways were also activated by VEGF, although the activation time and gene sets belonging to the pathways differed. In addition, HMGB-1 upregulated some VEGFR signaling-related angiogenic factors including EGR1 and, importantly, novel angiogenic factors, such as ABL2, CEACAM1, KIT, and VIPR1, which are reported to independently promote angiogenesis under physiological and pathological conditions. Our findings suggest that HMGB-1 independently induces angiogenesis by activating HMGB-1-specific angiogenic factors and also functions as an accelerator for VEGF-mediated conventional angiogenesis. |
format | Online Article Text |
id | pubmed-5720474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-57204742017-12-12 Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis Kim, Won Kyu Kwon, Yujin Park, Minhee Yun, Seongju Kwon, Ja-Young Kim, Hoguen BMB Rep Articles High-mobility group box-1 (HMGB-1) is expressed in almost all cells, and its dysregulated expression correlates with inflammatory diseases, ischemia, and cancer. Some of these conditions accompany HMGB-1-mediated abnormal angiogenesis. Thus far, the mechanism of HMGB-1-induced angiogenesis remains largely unknown. In this study, we performed time-dependent DNA microarray analysis of endothelial cells (ECs) after HMGB-1 or VEGF treatment. The pathway analysis of each gene set upregulated by HMGB-1 or VEGF showed that most HMGB-1-induced angiogenic pathways were also activated by VEGF, although the activation time and gene sets belonging to the pathways differed. In addition, HMGB-1 upregulated some VEGFR signaling-related angiogenic factors including EGR1 and, importantly, novel angiogenic factors, such as ABL2, CEACAM1, KIT, and VIPR1, which are reported to independently promote angiogenesis under physiological and pathological conditions. Our findings suggest that HMGB-1 independently induces angiogenesis by activating HMGB-1-specific angiogenic factors and also functions as an accelerator for VEGF-mediated conventional angiogenesis. Korean Society for Biochemistry and Molecular Biology 2017-11 2017-11-30 /pmc/articles/PMC5720474/ /pubmed/29065965 http://dx.doi.org/10.5483/BMBRep.2017.50.11.129 Text en Copyright © 2017 by the The Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Kim, Won Kyu Kwon, Yujin Park, Minhee Yun, Seongju Kwon, Ja-Young Kim, Hoguen Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis |
title | Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis |
title_full | Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis |
title_fullStr | Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis |
title_full_unstemmed | Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis |
title_short | Identification of specifically activated angiogenic molecules in HMGB-1-induced angiogenesis |
title_sort | identification of specifically activated angiogenic molecules in hmgb-1-induced angiogenesis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720474/ https://www.ncbi.nlm.nih.gov/pubmed/29065965 http://dx.doi.org/10.5483/BMBRep.2017.50.11.129 |
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