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Monitoring wound healing in a 3D wound model by hyperspectral imaging and efficient clustering
Wound healing is a complex and dynamic process with different distinct and overlapping phases from homeostasis, inflammation and proliferation to remodelling. Monitoring the healing response of injured tissue is of high importance for basic research and clinical practice. In traditional application,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720791/ https://www.ncbi.nlm.nih.gov/pubmed/29216188 http://dx.doi.org/10.1371/journal.pone.0186425 |
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author | Wahabzada, Mirwaes Besser, Manuela Khosravani, Milad Kuska, Matheus Thomas Kersting, Kristian Mahlein, Anne-Katrin Stürmer, Ewa |
author_facet | Wahabzada, Mirwaes Besser, Manuela Khosravani, Milad Kuska, Matheus Thomas Kersting, Kristian Mahlein, Anne-Katrin Stürmer, Ewa |
author_sort | Wahabzada, Mirwaes |
collection | PubMed |
description | Wound healing is a complex and dynamic process with different distinct and overlapping phases from homeostasis, inflammation and proliferation to remodelling. Monitoring the healing response of injured tissue is of high importance for basic research and clinical practice. In traditional application, biological markers characterize normal and abnormal wound healing. Understanding functional relationships of these biological processes is essential for developing new treatment strategies. However, most of the present techniques (in vitro or in vivo) include invasive microscopic or analytical tissue sampling. In the present study, a non-invasive alternative for monitoring processes during wound healing is introduced. Within this context, hyperspectral imaging (HSI) is an emerging and innovative non-invasive imaging technique with different opportunities in medical applications. HSI acquires the spectral reflectance of an object, depending on its biochemical and structural characteristics. An in-vitro 3-dimensional (3-D) wound model was established and incubated without and with acute and chronic wound fluid (AWF, CWF), respectively. Hyperspectral images of each individual specimen of this 3-D wound model were assessed at day 0/5/10 in vitro, and reflectance spectra were evaluated. For analysing the complex hyperspectral data, an efficient unsupervised approach for clustering massive hyperspectral data was designed, based on efficient hierarchical decomposition of spectral information according to archetypal data points. It represents, to the best of our knowledge, the first application of an advanced Data Mining approach in context of non-invasive analysis of wounds using hyperspectral imagery. By this, temporal and spatial pattern of hyperspectral clusters were determined within the tissue discs and among the different treatments. Results from non-invasive imaging were compared to the number of cells in the various clusters, assessed by Hematoxylin/Eosin (H/E) staining. It was possible to correlate cell quantity and spectral reflectance during wound closure in a 3-D wound model in vitro. |
format | Online Article Text |
id | pubmed-5720791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57207912017-12-15 Monitoring wound healing in a 3D wound model by hyperspectral imaging and efficient clustering Wahabzada, Mirwaes Besser, Manuela Khosravani, Milad Kuska, Matheus Thomas Kersting, Kristian Mahlein, Anne-Katrin Stürmer, Ewa PLoS One Research Article Wound healing is a complex and dynamic process with different distinct and overlapping phases from homeostasis, inflammation and proliferation to remodelling. Monitoring the healing response of injured tissue is of high importance for basic research and clinical practice. In traditional application, biological markers characterize normal and abnormal wound healing. Understanding functional relationships of these biological processes is essential for developing new treatment strategies. However, most of the present techniques (in vitro or in vivo) include invasive microscopic or analytical tissue sampling. In the present study, a non-invasive alternative for monitoring processes during wound healing is introduced. Within this context, hyperspectral imaging (HSI) is an emerging and innovative non-invasive imaging technique with different opportunities in medical applications. HSI acquires the spectral reflectance of an object, depending on its biochemical and structural characteristics. An in-vitro 3-dimensional (3-D) wound model was established and incubated without and with acute and chronic wound fluid (AWF, CWF), respectively. Hyperspectral images of each individual specimen of this 3-D wound model were assessed at day 0/5/10 in vitro, and reflectance spectra were evaluated. For analysing the complex hyperspectral data, an efficient unsupervised approach for clustering massive hyperspectral data was designed, based on efficient hierarchical decomposition of spectral information according to archetypal data points. It represents, to the best of our knowledge, the first application of an advanced Data Mining approach in context of non-invasive analysis of wounds using hyperspectral imagery. By this, temporal and spatial pattern of hyperspectral clusters were determined within the tissue discs and among the different treatments. Results from non-invasive imaging were compared to the number of cells in the various clusters, assessed by Hematoxylin/Eosin (H/E) staining. It was possible to correlate cell quantity and spectral reflectance during wound closure in a 3-D wound model in vitro. Public Library of Science 2017-12-07 /pmc/articles/PMC5720791/ /pubmed/29216188 http://dx.doi.org/10.1371/journal.pone.0186425 Text en © 2017 Wahabzada et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wahabzada, Mirwaes Besser, Manuela Khosravani, Milad Kuska, Matheus Thomas Kersting, Kristian Mahlein, Anne-Katrin Stürmer, Ewa Monitoring wound healing in a 3D wound model by hyperspectral imaging and efficient clustering |
title | Monitoring wound healing in a 3D wound model by hyperspectral imaging and efficient clustering |
title_full | Monitoring wound healing in a 3D wound model by hyperspectral imaging and efficient clustering |
title_fullStr | Monitoring wound healing in a 3D wound model by hyperspectral imaging and efficient clustering |
title_full_unstemmed | Monitoring wound healing in a 3D wound model by hyperspectral imaging and efficient clustering |
title_short | Monitoring wound healing in a 3D wound model by hyperspectral imaging and efficient clustering |
title_sort | monitoring wound healing in a 3d wound model by hyperspectral imaging and efficient clustering |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720791/ https://www.ncbi.nlm.nih.gov/pubmed/29216188 http://dx.doi.org/10.1371/journal.pone.0186425 |
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