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Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis

OBJECTIVES: Fecal calprotectin (FC) is widely used to monitor the activity of inflammatory bowel disease (IBD) and to tailor medical treatment to disease activity. Laboratory testing of fecal samples may have a turnaround time of 1–2 weeks, whereas FC home testing allows results within hours and thu...

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Autores principales: Hejl, Julie, Theede, Klaus, Møllgren, Brian, Madsen, Kirsten Vikkelsø, Heidari, Ashraf, á Steig, Anna, Fenger, Mogens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721267/
https://www.ncbi.nlm.nih.gov/pubmed/29234707
http://dx.doi.org/10.1016/j.plabm.2017.11.002
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author Hejl, Julie
Theede, Klaus
Møllgren, Brian
Madsen, Kirsten Vikkelsø
Heidari, Ashraf
á Steig, Anna
Fenger, Mogens
author_facet Hejl, Julie
Theede, Klaus
Møllgren, Brian
Madsen, Kirsten Vikkelsø
Heidari, Ashraf
á Steig, Anna
Fenger, Mogens
author_sort Hejl, Julie
collection PubMed
description OBJECTIVES: Fecal calprotectin (FC) is widely used to monitor the activity of inflammatory bowel disease (IBD) and to tailor medical treatment to disease activity. Laboratory testing of fecal samples may have a turnaround time of 1–2 weeks, whereas FC home testing allows results within hours and thus enables a rapid response to clinical deterioration. DESIGN AND METHODS: Fifty-five stool samples were analyzed by the IBDoc(®) Calprotectin Home Testing kit and the BÜHLMANN fCAL(®) turbo assay on a Roche Cobas 6000 c501. The correlation between the assays was assessed using Spearman's Rho correlation coefficient and the intermediate imprecision of both assays was calculated. RESULTS: We found a strong correlation coefficient of 0.887 between FC measured on IBDoc® and the laboratory assay BÜHLMANN fCAL(®) turbo. The coefficients of variation (CVs) at three different FC levels were in the range 2.3–5.5% (BÜHLMANN fCAL(®) turbo) and in the range of 4.8–26.6% (IBDoc(®)). CONCLUSIONS: This study suggests that IBDoc® is a suitable alternative for the assessment of disease activity in IBD patients.
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spelling pubmed-57212672017-12-11 Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis Hejl, Julie Theede, Klaus Møllgren, Brian Madsen, Kirsten Vikkelsø Heidari, Ashraf á Steig, Anna Fenger, Mogens Pract Lab Med Article OBJECTIVES: Fecal calprotectin (FC) is widely used to monitor the activity of inflammatory bowel disease (IBD) and to tailor medical treatment to disease activity. Laboratory testing of fecal samples may have a turnaround time of 1–2 weeks, whereas FC home testing allows results within hours and thus enables a rapid response to clinical deterioration. DESIGN AND METHODS: Fifty-five stool samples were analyzed by the IBDoc(®) Calprotectin Home Testing kit and the BÜHLMANN fCAL(®) turbo assay on a Roche Cobas 6000 c501. The correlation between the assays was assessed using Spearman's Rho correlation coefficient and the intermediate imprecision of both assays was calculated. RESULTS: We found a strong correlation coefficient of 0.887 between FC measured on IBDoc® and the laboratory assay BÜHLMANN fCAL(®) turbo. The coefficients of variation (CVs) at three different FC levels were in the range 2.3–5.5% (BÜHLMANN fCAL(®) turbo) and in the range of 4.8–26.6% (IBDoc(®)). CONCLUSIONS: This study suggests that IBDoc® is a suitable alternative for the assessment of disease activity in IBD patients. Elsevier 2017-11-21 /pmc/articles/PMC5721267/ /pubmed/29234707 http://dx.doi.org/10.1016/j.plabm.2017.11.002 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Hejl, Julie
Theede, Klaus
Møllgren, Brian
Madsen, Kirsten Vikkelsø
Heidari, Ashraf
á Steig, Anna
Fenger, Mogens
Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis
title Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis
title_full Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis
title_fullStr Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis
title_full_unstemmed Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis
title_short Point of care testing of fecal calprotectin as a substitute for routine laboratory analysis
title_sort point of care testing of fecal calprotectin as a substitute for routine laboratory analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721267/
https://www.ncbi.nlm.nih.gov/pubmed/29234707
http://dx.doi.org/10.1016/j.plabm.2017.11.002
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