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Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli

In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands...

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Detalles Bibliográficos
Autores principales: Karadjian, Grégory, Heckmann, Aurélie, Rosa, Giuseppe La, Pozio, Edoardo, Boireau, Pascal, Vallée, Isabelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721686/
https://www.ncbi.nlm.nih.gov/pubmed/29219110
http://dx.doi.org/10.1051/parasite/2017053
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author Karadjian, Grégory
Heckmann, Aurélie
Rosa, Giuseppe La
Pozio, Edoardo
Boireau, Pascal
Vallée, Isabelle
author_facet Karadjian, Grégory
Heckmann, Aurélie
Rosa, Giuseppe La
Pozio, Edoardo
Boireau, Pascal
Vallée, Isabelle
author_sort Karadjian, Grégory
collection PubMed
description In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands of different sizes, resulting in a specific band pattern. By multiplex PCR, Trichinella murrelli shows two bands of 127 bp and 316 bp. However, a third band of 256 bp can occur. This band can lead to misidentification, since it is similar to the 253 bp band displayed by Trichinella britovi. BLAST analysis confirmed that the 256 bp band is from T. murrelli. The aim of this short note is to inform analysts that T. murrelli larvae may display either two- or three-band patterns.
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spelling pubmed-57216862017-12-19 Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli Karadjian, Grégory Heckmann, Aurélie Rosa, Giuseppe La Pozio, Edoardo Boireau, Pascal Vallée, Isabelle Parasite Short Note In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands of different sizes, resulting in a specific band pattern. By multiplex PCR, Trichinella murrelli shows two bands of 127 bp and 316 bp. However, a third band of 256 bp can occur. This band can lead to misidentification, since it is similar to the 253 bp band displayed by Trichinella britovi. BLAST analysis confirmed that the 256 bp band is from T. murrelli. The aim of this short note is to inform analysts that T. murrelli larvae may display either two- or three-band patterns. EDP Sciences 2017-12-08 /pmc/articles/PMC5721686/ /pubmed/29219110 http://dx.doi.org/10.1051/parasite/2017053 Text en © G. Karadjian et al., published by EDP Sciences, 2017 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Note
Karadjian, Grégory
Heckmann, Aurélie
Rosa, Giuseppe La
Pozio, Edoardo
Boireau, Pascal
Vallée, Isabelle
Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli
title Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli
title_full Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli
title_fullStr Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli
title_full_unstemmed Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli
title_short Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli
title_sort molecular identification of trichinella species by multiplex pcr: new insight for trichinella murrelli
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721686/
https://www.ncbi.nlm.nih.gov/pubmed/29219110
http://dx.doi.org/10.1051/parasite/2017053
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