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Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke

BACKGROUND: Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukoc...

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Autores principales: Lien, Li‐Ming, Chiou, Hung‐Yi, Yeh, Hsu‐Ling, Chiu, Shang‐Yen, Jeng, Jiann‐Shing, Lin, Huey‐Juan, Hu, Chaur‐Jong, Hsieh, Fang‐I, Wei, Yau‐Huei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721740/
https://www.ncbi.nlm.nih.gov/pubmed/29151031
http://dx.doi.org/10.1161/JAHA.117.006157
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author Lien, Li‐Ming
Chiou, Hung‐Yi
Yeh, Hsu‐Ling
Chiu, Shang‐Yen
Jeng, Jiann‐Shing
Lin, Huey‐Juan
Hu, Chaur‐Jong
Hsieh, Fang‐I
Wei, Yau‐Huei
author_facet Lien, Li‐Ming
Chiou, Hung‐Yi
Yeh, Hsu‐Ling
Chiu, Shang‐Yen
Jeng, Jiann‐Shing
Lin, Huey‐Juan
Hu, Chaur‐Jong
Hsieh, Fang‐I
Wei, Yau‐Huei
author_sort Lien, Li‐Ming
collection PubMed
description BACKGROUND: Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. METHODS AND RESULTS: A total of 350 patients with first‐ever ischemic stroke and 350 healthy controls were recruited in this case‐control study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real‐time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8‐hydroxy‐2′‐deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8‐hydroxy‐2′‐deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. CONCLUSIONS: Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke.
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spelling pubmed-57217402017-12-12 Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke Lien, Li‐Ming Chiou, Hung‐Yi Yeh, Hsu‐Ling Chiu, Shang‐Yen Jeng, Jiann‐Shing Lin, Huey‐Juan Hu, Chaur‐Jong Hsieh, Fang‐I Wei, Yau‐Huei J Am Heart Assoc Original Research BACKGROUND: Cumulative evidence has shown that low mitochondrial DNA (mtDNA) content is related to elevated oxidative stress and atherosclerosis, which play important roles in ischemic stroke. The objective of this study was to explore the association between mtDNA content in peripheral blood leukocytes and ischemic stroke. METHODS AND RESULTS: A total of 350 patients with first‐ever ischemic stroke and 350 healthy controls were recruited in this case‐control study. The mtDNA content in peripheral blood leukocytes was determined by quantitative real‐time polymerase chain reaction. The levels of oxidized glutathione, reduced glutathione, and 8‐hydroxy‐2′‐deoxyguanosine were measured by ELISA kits. Multivariate logistic regression models were used to analyze the relationship between mtDNA content in peripheral blood leukocytes and ischemic stroke. Our results show that mtDNA content of patients with ischemic stroke was notably lower compared with controls. A significant association was found between low mtDNA content and ischemic stroke. Furthermore, significant interactions were identified between low mtDNA and proven risk factors in patients with ischemic stroke. The levels of oxidized glutathione and 8‐hydroxy‐2′‐deoxyguanosine were significantly greater in patients with ischemic stroke compared with controls. CONCLUSIONS: Our results demonstrate that low mtDNA content in peripheral blood leukocytes is associated with ischemic stroke. The relationship of low mtDNA content and ischemic stroke was particularly notable in individuals who had low mtDNA content combined with diabetes mellitus, metabolic syndrome, or cigarette smoking. Oxidative stress may be one of the contributory factors to decreased mtDNA content in patients with ischemic stroke. John Wiley and Sons Inc. 2017-11-18 /pmc/articles/PMC5721740/ /pubmed/29151031 http://dx.doi.org/10.1161/JAHA.117.006157 Text en © 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Lien, Li‐Ming
Chiou, Hung‐Yi
Yeh, Hsu‐Ling
Chiu, Shang‐Yen
Jeng, Jiann‐Shing
Lin, Huey‐Juan
Hu, Chaur‐Jong
Hsieh, Fang‐I
Wei, Yau‐Huei
Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke
title Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke
title_full Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke
title_fullStr Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke
title_full_unstemmed Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke
title_short Significant Association Between Low Mitochondrial DNA Content in Peripheral Blood Leukocytes and Ischemic Stroke
title_sort significant association between low mitochondrial dna content in peripheral blood leukocytes and ischemic stroke
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721740/
https://www.ncbi.nlm.nih.gov/pubmed/29151031
http://dx.doi.org/10.1161/JAHA.117.006157
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