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Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System
BACKGROUND: Hormesis is considered a dose–response phenomenon characterized by growth stimulation at low doses and inhibition at high doses. The hormetic response by silver nanoparticles (AgNPs) on in vitro multiplication of sugarcane was evaluated using a temporary immersion system. METHODS: Sugarc...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721974/ https://www.ncbi.nlm.nih.gov/pubmed/29238274 http://dx.doi.org/10.1177/1559325817744945 |
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author | Bello-Bello, Jericó J. Chavez-Santoscoy, Rocío A. Lecona-Guzmán, Carlos A. Bogdanchikova, Nina Salinas-Ruíz, Josafhat Gómez-Merino, Fernando Carlos Pestryakov, Alexey |
author_facet | Bello-Bello, Jericó J. Chavez-Santoscoy, Rocío A. Lecona-Guzmán, Carlos A. Bogdanchikova, Nina Salinas-Ruíz, Josafhat Gómez-Merino, Fernando Carlos Pestryakov, Alexey |
author_sort | Bello-Bello, Jericó J. |
collection | PubMed |
description | BACKGROUND: Hormesis is considered a dose–response phenomenon characterized by growth stimulation at low doses and inhibition at high doses. The hormetic response by silver nanoparticles (AgNPs) on in vitro multiplication of sugarcane was evaluated using a temporary immersion system. METHODS: Sugarcane shoots were used as explants cultured in Murashige and Skoog medium with AgNPs at concentrations of 0, 25, 50, 100, and 200 mg/L. Shoot multiplication rate and length were used to determine hormetic response. Total content of phenolic compounds of sugarcane, mineral nutrition, and reactive oxygen species (ROS) was determined. RESULTS: Results were presented as a dose–response curve. Stimulation phase growth was observed at 50 mg/L AgNPs, whereas inhibition phase was detected at 200 mg/L AgNPs. Mineral nutrient analysis showed changes in macronutrient and micronutrient contents due to the effect of AgNPs. Moreover, AgNPs induced ROS production and increased total phenolic content, with a dose-dependent effect. CONCLUSION: Results suggested that the production of ROS and mineral nutrition are key mechanisms of AgNP-induced hormesis and that phenolic accumulation was obtained as a response of the plant to stress produced by high doses of AgNPs. Therefore, small doses of AgNPs in the culture medium could be an efficient strategy for commercial micropropagation. |
format | Online Article Text |
id | pubmed-5721974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-57219742017-12-13 Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System Bello-Bello, Jericó J. Chavez-Santoscoy, Rocío A. Lecona-Guzmán, Carlos A. Bogdanchikova, Nina Salinas-Ruíz, Josafhat Gómez-Merino, Fernando Carlos Pestryakov, Alexey Dose Response Original Article BACKGROUND: Hormesis is considered a dose–response phenomenon characterized by growth stimulation at low doses and inhibition at high doses. The hormetic response by silver nanoparticles (AgNPs) on in vitro multiplication of sugarcane was evaluated using a temporary immersion system. METHODS: Sugarcane shoots were used as explants cultured in Murashige and Skoog medium with AgNPs at concentrations of 0, 25, 50, 100, and 200 mg/L. Shoot multiplication rate and length were used to determine hormetic response. Total content of phenolic compounds of sugarcane, mineral nutrition, and reactive oxygen species (ROS) was determined. RESULTS: Results were presented as a dose–response curve. Stimulation phase growth was observed at 50 mg/L AgNPs, whereas inhibition phase was detected at 200 mg/L AgNPs. Mineral nutrient analysis showed changes in macronutrient and micronutrient contents due to the effect of AgNPs. Moreover, AgNPs induced ROS production and increased total phenolic content, with a dose-dependent effect. CONCLUSION: Results suggested that the production of ROS and mineral nutrition are key mechanisms of AgNP-induced hormesis and that phenolic accumulation was obtained as a response of the plant to stress produced by high doses of AgNPs. Therefore, small doses of AgNPs in the culture medium could be an efficient strategy for commercial micropropagation. SAGE Publications 2017-12-04 /pmc/articles/PMC5721974/ /pubmed/29238274 http://dx.doi.org/10.1177/1559325817744945 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Bello-Bello, Jericó J. Chavez-Santoscoy, Rocío A. Lecona-Guzmán, Carlos A. Bogdanchikova, Nina Salinas-Ruíz, Josafhat Gómez-Merino, Fernando Carlos Pestryakov, Alexey Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System |
title | Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System |
title_full | Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System |
title_fullStr | Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System |
title_full_unstemmed | Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System |
title_short | Hormetic Response by Silver Nanoparticles on In Vitro Multiplication of Sugarcane (Saccharum spp. Cv. Mex 69-290) Using a Temporary Immersion System |
title_sort | hormetic response by silver nanoparticles on in vitro multiplication of sugarcane (saccharum spp. cv. mex 69-290) using a temporary immersion system |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721974/ https://www.ncbi.nlm.nih.gov/pubmed/29238274 http://dx.doi.org/10.1177/1559325817744945 |
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