Cargando…

Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65

OBJECTIVE: This study was designed to investigate the effect of combination of ursolic acid (UA) with cisplatin (DDP) on cervical cancer cell proliferation and apoptosis. METHODS: The mRNA and protein expressions of nuclear factor-kappa B (NF-κB) p65 in cervical cancer cells were examined using RT-P...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Lan, Hou, Yu, Yu, Jing, Lu, Yulin, Chang, Li, Jiang, Meiping, Wu, Xingrao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5722573/
https://www.ncbi.nlm.nih.gov/pubmed/29228621
http://dx.doi.org/10.18632/oncotarget.22133
_version_ 1783285044654112768
author Li, Lan
Hou, Yu
Yu, Jing
Lu, Yulin
Chang, Li
Jiang, Meiping
Wu, Xingrao
author_facet Li, Lan
Hou, Yu
Yu, Jing
Lu, Yulin
Chang, Li
Jiang, Meiping
Wu, Xingrao
author_sort Li, Lan
collection PubMed
description OBJECTIVE: This study was designed to investigate the effect of combination of ursolic acid (UA) with cisplatin (DDP) on cervical cancer cell proliferation and apoptosis. METHODS: The mRNA and protein expressions of nuclear factor-kappa B (NF-κB) p65 in cervical cancer cells were examined using RT-PCR and western blot. MTT and colony formation assays were performed to examine the DDP toxicity and the proliferation ability of cervical cancer cells. Cell morphology was observed by means of Hoechst33258 and transmission electron microscopy (TEM). The apoptosis rate and cell cycle were assessed through flow cytometry assay. Western blot was used to detect the expression of apoptosis-related molecules. RESULTS: The mRNA and protein expressions of NF-κB p65 in cervical cancer cells were significantly higher than that in cervical epithelial cells. The combined treatment of UA and DDP inhibited cervical cancer cell growth and promoted apoptosis more effectively than DDP treatment or UA treatment alone (P < 0.05). Compared with the DDP group and UA group, the expressions of Bcl-2 and NF-κB p65 in DDP +UA group were decreased, while the expressions of Bax, Caspase-3 and PARP cleavage were observably increased. The expression of nuclear NF-κB p65 significantly reduced in UA group and DDP +UA group. si-p65 group displayed a decrease of cell proliferation ability and led to a significant reduction in the number of SiHa cell colony formation. CONCLUSION: The combination of UA with DDP could more effectively inhibit SiHa cells proliferation and facilitate cell apoptosis through suppressing NF-κB p65.
format Online
Article
Text
id pubmed-5722573
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-57225732017-12-10 Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65 Li, Lan Hou, Yu Yu, Jing Lu, Yulin Chang, Li Jiang, Meiping Wu, Xingrao Oncotarget Research Paper OBJECTIVE: This study was designed to investigate the effect of combination of ursolic acid (UA) with cisplatin (DDP) on cervical cancer cell proliferation and apoptosis. METHODS: The mRNA and protein expressions of nuclear factor-kappa B (NF-κB) p65 in cervical cancer cells were examined using RT-PCR and western blot. MTT and colony formation assays were performed to examine the DDP toxicity and the proliferation ability of cervical cancer cells. Cell morphology was observed by means of Hoechst33258 and transmission electron microscopy (TEM). The apoptosis rate and cell cycle were assessed through flow cytometry assay. Western blot was used to detect the expression of apoptosis-related molecules. RESULTS: The mRNA and protein expressions of NF-κB p65 in cervical cancer cells were significantly higher than that in cervical epithelial cells. The combined treatment of UA and DDP inhibited cervical cancer cell growth and promoted apoptosis more effectively than DDP treatment or UA treatment alone (P < 0.05). Compared with the DDP group and UA group, the expressions of Bcl-2 and NF-κB p65 in DDP +UA group were decreased, while the expressions of Bax, Caspase-3 and PARP cleavage were observably increased. The expression of nuclear NF-κB p65 significantly reduced in UA group and DDP +UA group. si-p65 group displayed a decrease of cell proliferation ability and led to a significant reduction in the number of SiHa cell colony formation. CONCLUSION: The combination of UA with DDP could more effectively inhibit SiHa cells proliferation and facilitate cell apoptosis through suppressing NF-κB p65. Impact Journals LLC 2017-10-30 /pmc/articles/PMC5722573/ /pubmed/29228621 http://dx.doi.org/10.18632/oncotarget.22133 Text en Copyright: © 2017 Li et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Li, Lan
Hou, Yu
Yu, Jing
Lu, Yulin
Chang, Li
Jiang, Meiping
Wu, Xingrao
Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65
title Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65
title_full Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65
title_fullStr Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65
title_full_unstemmed Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65
title_short Synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing NF-κB p65
title_sort synergism of ursolic acid and cisplatin promotes apoptosis and enhances growth inhibition of cervical cancer cells via suppressing nf-κb p65
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5722573/
https://www.ncbi.nlm.nih.gov/pubmed/29228621
http://dx.doi.org/10.18632/oncotarget.22133
work_keys_str_mv AT lilan synergismofursolicacidandcisplatinpromotesapoptosisandenhancesgrowthinhibitionofcervicalcancercellsviasuppressingnfkbp65
AT houyu synergismofursolicacidandcisplatinpromotesapoptosisandenhancesgrowthinhibitionofcervicalcancercellsviasuppressingnfkbp65
AT yujing synergismofursolicacidandcisplatinpromotesapoptosisandenhancesgrowthinhibitionofcervicalcancercellsviasuppressingnfkbp65
AT luyulin synergismofursolicacidandcisplatinpromotesapoptosisandenhancesgrowthinhibitionofcervicalcancercellsviasuppressingnfkbp65
AT changli synergismofursolicacidandcisplatinpromotesapoptosisandenhancesgrowthinhibitionofcervicalcancercellsviasuppressingnfkbp65
AT jiangmeiping synergismofursolicacidandcisplatinpromotesapoptosisandenhancesgrowthinhibitionofcervicalcancercellsviasuppressingnfkbp65
AT wuxingrao synergismofursolicacidandcisplatinpromotesapoptosisandenhancesgrowthinhibitionofcervicalcancercellsviasuppressingnfkbp65