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GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

BACKGROUND: State-of-the-art strain engineering techniques for the host Pichia pastoris (syn. Komagataella spp.) include overexpression of homologous and heterologous genes, and deletion of host genes. For metabolic and cell engineering purposes the simultaneous overexpression of more than one gene...

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Detalles Bibliográficos
Autores principales:	Prielhofer, Roland, Barrero, Juan J., Steuer, Stefanie, Gassler, Thomas, Zahrl, Richard, Baumann, Kristin, Sauer, Michael, Mattanovich, Diethard, Gasser, Brigitte, Marx, Hans
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723102/ https://www.ncbi.nlm.nih.gov/pubmed/29221460 http://dx.doi.org/10.1186/s12918-017-0492-3

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