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Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques

Herpes simplex virus 1 and 2 (HSV-1/2) similarly initiate infection in mucosal epithelia and establish lifelong neuronal latency. Anogenital HSV-2 infection augments the risk for sexual human immunodeficiency virus (HIV) transmission and is associated with higher HIV viral loads. However, whether or...

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Autores principales: Aravantinou, Meropi, Mizenina, Olga, Calenda, Giulia, Kenney, Jessica, Frank, Ines, Lifson, Jeffrey D., Szpara, Moriah, Jing, Lichen, Koelle, David M., Teleshova, Natalia, Grasperge, Brooke, Blanchard, James, Gettie, Agegnehu, Martinelli, Elena, Derby, Nina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723348/
https://www.ncbi.nlm.nih.gov/pubmed/29259582
http://dx.doi.org/10.3389/fmicb.2017.02342
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author Aravantinou, Meropi
Mizenina, Olga
Calenda, Giulia
Kenney, Jessica
Frank, Ines
Lifson, Jeffrey D.
Szpara, Moriah
Jing, Lichen
Koelle, David M.
Teleshova, Natalia
Grasperge, Brooke
Blanchard, James
Gettie, Agegnehu
Martinelli, Elena
Derby, Nina
author_facet Aravantinou, Meropi
Mizenina, Olga
Calenda, Giulia
Kenney, Jessica
Frank, Ines
Lifson, Jeffrey D.
Szpara, Moriah
Jing, Lichen
Koelle, David M.
Teleshova, Natalia
Grasperge, Brooke
Blanchard, James
Gettie, Agegnehu
Martinelli, Elena
Derby, Nina
author_sort Aravantinou, Meropi
collection PubMed
description Herpes simplex virus 1 and 2 (HSV-1/2) similarly initiate infection in mucosal epithelia and establish lifelong neuronal latency. Anogenital HSV-2 infection augments the risk for sexual human immunodeficiency virus (HIV) transmission and is associated with higher HIV viral loads. However, whether oral HSV-1 infection contributes to oral HIV susceptibility, viremia, or oral complications of HIV infection is unknown. Appropriate non-human primate (NHP) models would facilitate this investigation, yet there are no published studies of HSV-1/SIV co-infection in NHPs. Thus, we performed a pilot study for an oral HSV-1 infection model in SIV-infected rhesus macaques to describe the feasibility of the modeling and resultant immunological changes. Three SIV-infected, clinically healthy macaques became HSV-1-infected by inoculation with 4 × 10(8) pfu HSV-1 McKrae on buccal, tongue, gingiva, and tonsils after gentle abrasion. HSV-1 DNA was shed in oral swabs for up to 21 days, and shedding recurred in association with intra-oral lesions after periods of no shedding during 56 days of follow up. HSV-1 DNA was detected in explant cultures of trigeminal ganglia collected at euthanasia on day 56. In the macaque with lowest baseline SIV viremia, SIV plasma RNA increased following HSV-1 infection. One macaque exhibited an acute pro-inflammatory response, and all three animals experienced T cell activation and mobilization in blood. However, T cell and antibody responses to HSV-1 were low and atypical. Through rigorous assessesments, this study finds that the virulent HSV-1 strain McKrae resulted in a low level HSV-1 infection that elicited modest immune responses and transiently modulated SIV infection.
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spelling pubmed-57233482017-12-19 Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques Aravantinou, Meropi Mizenina, Olga Calenda, Giulia Kenney, Jessica Frank, Ines Lifson, Jeffrey D. Szpara, Moriah Jing, Lichen Koelle, David M. Teleshova, Natalia Grasperge, Brooke Blanchard, James Gettie, Agegnehu Martinelli, Elena Derby, Nina Front Microbiol Microbiology Herpes simplex virus 1 and 2 (HSV-1/2) similarly initiate infection in mucosal epithelia and establish lifelong neuronal latency. Anogenital HSV-2 infection augments the risk for sexual human immunodeficiency virus (HIV) transmission and is associated with higher HIV viral loads. However, whether oral HSV-1 infection contributes to oral HIV susceptibility, viremia, or oral complications of HIV infection is unknown. Appropriate non-human primate (NHP) models would facilitate this investigation, yet there are no published studies of HSV-1/SIV co-infection in NHPs. Thus, we performed a pilot study for an oral HSV-1 infection model in SIV-infected rhesus macaques to describe the feasibility of the modeling and resultant immunological changes. Three SIV-infected, clinically healthy macaques became HSV-1-infected by inoculation with 4 × 10(8) pfu HSV-1 McKrae on buccal, tongue, gingiva, and tonsils after gentle abrasion. HSV-1 DNA was shed in oral swabs for up to 21 days, and shedding recurred in association with intra-oral lesions after periods of no shedding during 56 days of follow up. HSV-1 DNA was detected in explant cultures of trigeminal ganglia collected at euthanasia on day 56. In the macaque with lowest baseline SIV viremia, SIV plasma RNA increased following HSV-1 infection. One macaque exhibited an acute pro-inflammatory response, and all three animals experienced T cell activation and mobilization in blood. However, T cell and antibody responses to HSV-1 were low and atypical. Through rigorous assessesments, this study finds that the virulent HSV-1 strain McKrae resulted in a low level HSV-1 infection that elicited modest immune responses and transiently modulated SIV infection. Frontiers Media S.A. 2017-12-05 /pmc/articles/PMC5723348/ /pubmed/29259582 http://dx.doi.org/10.3389/fmicb.2017.02342 Text en Copyright © 2017 Aravantinou, Mizenina, Calenda, Kenney, Frank, Lifson, Szpara, Jing, Koelle, Teleshova, Grasperge, Blanchard, Gettie, Martinelli and Derby. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Aravantinou, Meropi
Mizenina, Olga
Calenda, Giulia
Kenney, Jessica
Frank, Ines
Lifson, Jeffrey D.
Szpara, Moriah
Jing, Lichen
Koelle, David M.
Teleshova, Natalia
Grasperge, Brooke
Blanchard, James
Gettie, Agegnehu
Martinelli, Elena
Derby, Nina
Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques
title Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques
title_full Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques
title_fullStr Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques
title_full_unstemmed Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques
title_short Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques
title_sort experimental oral herpes simplex virus-1 (hsv-1) co-infection in simian immunodeficiency virus (siv)-infected rhesus macaques
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723348/
https://www.ncbi.nlm.nih.gov/pubmed/29259582
http://dx.doi.org/10.3389/fmicb.2017.02342
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