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A comprehensive study of eco-friendly natural pigment and its applications
Actinomycetes, a large group of filamentous bacteria account for 70–80% of secondary metabolites available commercially. The present investigation was undertaken with an aim to identify and characterize pigment from actinomycetes. Actinomycetes were isolated from rhizosphere soil samples collected f...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723356/ https://www.ncbi.nlm.nih.gov/pubmed/29234729 http://dx.doi.org/10.1016/j.bbrep.2017.11.002 |
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author | Parmar, Ramendra Singh Singh, Charu |
author_facet | Parmar, Ramendra Singh Singh, Charu |
author_sort | Parmar, Ramendra Singh |
collection | PubMed |
description | Actinomycetes, a large group of filamentous bacteria account for 70–80% of secondary metabolites available commercially. The present investigation was undertaken with an aim to identify and characterize pigment from actinomycetes. Actinomycetes were isolated from rhizosphere soil samples collected from different regions of Madhya Pradesh state. Out of 85 actinomycetes, only 5 actinomycetes showed pigment production and based on diffusible pigment production ability one actinomycete ARITM02 was selected. The extraction of pigment was done by solvent extraction method using methanol and purified by TLC and column chromatography. The pigment was characterized by UV–Vis spectroscopy which showed the lamda maximum of 277.44. FTIR spectroscopy suggested various functional groups like amino group, amide group, hydroxide, benzene and lactone group. The Mass spectroscopy and NMR spectroscopy showed that the molecular mass of pigment is 621.7 and molecular formula is C34H43N3O8. The pigment was also tested for Antimicrobial activity against broad spectrum human pathogens, antioxidant test and toxicity test for safe use as a natural colorant in cosmetic, food, pharmaceutical and textile industries. The conclusion of study suggested that this novel pigment could be a versatile natural, safe and multipurpose. |
format | Online Article Text |
id | pubmed-5723356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-57233562017-12-11 A comprehensive study of eco-friendly natural pigment and its applications Parmar, Ramendra Singh Singh, Charu Biochem Biophys Rep Research Article Actinomycetes, a large group of filamentous bacteria account for 70–80% of secondary metabolites available commercially. The present investigation was undertaken with an aim to identify and characterize pigment from actinomycetes. Actinomycetes were isolated from rhizosphere soil samples collected from different regions of Madhya Pradesh state. Out of 85 actinomycetes, only 5 actinomycetes showed pigment production and based on diffusible pigment production ability one actinomycete ARITM02 was selected. The extraction of pigment was done by solvent extraction method using methanol and purified by TLC and column chromatography. The pigment was characterized by UV–Vis spectroscopy which showed the lamda maximum of 277.44. FTIR spectroscopy suggested various functional groups like amino group, amide group, hydroxide, benzene and lactone group. The Mass spectroscopy and NMR spectroscopy showed that the molecular mass of pigment is 621.7 and molecular formula is C34H43N3O8. The pigment was also tested for Antimicrobial activity against broad spectrum human pathogens, antioxidant test and toxicity test for safe use as a natural colorant in cosmetic, food, pharmaceutical and textile industries. The conclusion of study suggested that this novel pigment could be a versatile natural, safe and multipurpose. Elsevier 2017-12-01 /pmc/articles/PMC5723356/ /pubmed/29234729 http://dx.doi.org/10.1016/j.bbrep.2017.11.002 Text en © 2017 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Parmar, Ramendra Singh Singh, Charu A comprehensive study of eco-friendly natural pigment and its applications |
title | A comprehensive study of eco-friendly natural pigment and its applications |
title_full | A comprehensive study of eco-friendly natural pigment and its applications |
title_fullStr | A comprehensive study of eco-friendly natural pigment and its applications |
title_full_unstemmed | A comprehensive study of eco-friendly natural pigment and its applications |
title_short | A comprehensive study of eco-friendly natural pigment and its applications |
title_sort | comprehensive study of eco-friendly natural pigment and its applications |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723356/ https://www.ncbi.nlm.nih.gov/pubmed/29234729 http://dx.doi.org/10.1016/j.bbrep.2017.11.002 |
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