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A comprehensive study of eco-friendly natural pigment and its applications

Actinomycetes, a large group of filamentous bacteria account for 70–80% of secondary metabolites available commercially. The present investigation was undertaken with an aim to identify and characterize pigment from actinomycetes. Actinomycetes were isolated from rhizosphere soil samples collected f...

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Autores principales: Parmar, Ramendra Singh, Singh, Charu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723356/
https://www.ncbi.nlm.nih.gov/pubmed/29234729
http://dx.doi.org/10.1016/j.bbrep.2017.11.002
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author Parmar, Ramendra Singh
Singh, Charu
author_facet Parmar, Ramendra Singh
Singh, Charu
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description Actinomycetes, a large group of filamentous bacteria account for 70–80% of secondary metabolites available commercially. The present investigation was undertaken with an aim to identify and characterize pigment from actinomycetes. Actinomycetes were isolated from rhizosphere soil samples collected from different regions of Madhya Pradesh state. Out of 85 actinomycetes, only 5 actinomycetes showed pigment production and based on diffusible pigment production ability one actinomycete ARITM02 was selected. The extraction of pigment was done by solvent extraction method using methanol and purified by TLC and column chromatography. The pigment was characterized by UV–Vis spectroscopy which showed the lamda maximum of 277.44. FTIR spectroscopy suggested various functional groups like amino group, amide group, hydroxide, benzene and lactone group. The Mass spectroscopy and NMR spectroscopy showed that the molecular mass of pigment is 621.7 and molecular formula is C34H43N3O8. The pigment was also tested for Antimicrobial activity against broad spectrum human pathogens, antioxidant test and toxicity test for safe use as a natural colorant in cosmetic, food, pharmaceutical and textile industries. The conclusion of study suggested that this novel pigment could be a versatile natural, safe and multipurpose.
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spelling pubmed-57233562017-12-11 A comprehensive study of eco-friendly natural pigment and its applications Parmar, Ramendra Singh Singh, Charu Biochem Biophys Rep Research Article Actinomycetes, a large group of filamentous bacteria account for 70–80% of secondary metabolites available commercially. The present investigation was undertaken with an aim to identify and characterize pigment from actinomycetes. Actinomycetes were isolated from rhizosphere soil samples collected from different regions of Madhya Pradesh state. Out of 85 actinomycetes, only 5 actinomycetes showed pigment production and based on diffusible pigment production ability one actinomycete ARITM02 was selected. The extraction of pigment was done by solvent extraction method using methanol and purified by TLC and column chromatography. The pigment was characterized by UV–Vis spectroscopy which showed the lamda maximum of 277.44. FTIR spectroscopy suggested various functional groups like amino group, amide group, hydroxide, benzene and lactone group. The Mass spectroscopy and NMR spectroscopy showed that the molecular mass of pigment is 621.7 and molecular formula is C34H43N3O8. The pigment was also tested for Antimicrobial activity against broad spectrum human pathogens, antioxidant test and toxicity test for safe use as a natural colorant in cosmetic, food, pharmaceutical and textile industries. The conclusion of study suggested that this novel pigment could be a versatile natural, safe and multipurpose. Elsevier 2017-12-01 /pmc/articles/PMC5723356/ /pubmed/29234729 http://dx.doi.org/10.1016/j.bbrep.2017.11.002 Text en © 2017 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Parmar, Ramendra Singh
Singh, Charu
A comprehensive study of eco-friendly natural pigment and its applications
title A comprehensive study of eco-friendly natural pigment and its applications
title_full A comprehensive study of eco-friendly natural pigment and its applications
title_fullStr A comprehensive study of eco-friendly natural pigment and its applications
title_full_unstemmed A comprehensive study of eco-friendly natural pigment and its applications
title_short A comprehensive study of eco-friendly natural pigment and its applications
title_sort comprehensive study of eco-friendly natural pigment and its applications
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723356/
https://www.ncbi.nlm.nih.gov/pubmed/29234729
http://dx.doi.org/10.1016/j.bbrep.2017.11.002
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