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The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair

Combinations of fibroblasts (Fbs) and corresponding epithelial cells have been widely used in many tissues, such as the skin and breast tissues, to augment tissue repair and remodeling. Recently, a large amount of new data has indicated that nerve Fbs play critical roles in Schwann cells (SCs) and a...

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Autores principales: Wang, Yang, Li, Dong, Wang, Gangyang, Chen, Lulu, Chen, Jun, Liu, Zhangyin, Zhang, Zhaofeng, Shen, Hua, Jin, Yuqing, Shen, Zunli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723917/
https://www.ncbi.nlm.nih.gov/pubmed/29230099
http://dx.doi.org/10.7150/ijbs.21976
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author Wang, Yang
Li, Dong
Wang, Gangyang
Chen, Lulu
Chen, Jun
Liu, Zhangyin
Zhang, Zhaofeng
Shen, Hua
Jin, Yuqing
Shen, Zunli
author_facet Wang, Yang
Li, Dong
Wang, Gangyang
Chen, Lulu
Chen, Jun
Liu, Zhangyin
Zhang, Zhaofeng
Shen, Hua
Jin, Yuqing
Shen, Zunli
author_sort Wang, Yang
collection PubMed
description Combinations of fibroblasts (Fbs) and corresponding epithelial cells have been widely used in many tissues, such as the skin and breast tissues, to augment tissue repair and remodeling. Recently, a large amount of new data has indicated that nerve Fbs play critical roles in Schwann cells (SCs) and axons in vitro. However, little is known regarding the effects of co-transplanting nerve Fbs and SCs on peripheral nerve repair in vivo. The aim of this study was to investigate the effect of co-transplanting sciatic nerve Fbs (SN-Fbs) and sciatic nerve SCs (SN-SCs) on nerve regeneration. We developed a 5 mm nerve-defect model in mice using a polyurethane (PUR) catheter and then injected one of four different mixtures of cells into the catheters to form the following four groups: pure Matrigel (Control group), SN-Fbs alone (SN-Fb group), SN-Fbs combined with SN-SCs at a ratio of 1:2 (Fb&SC group) and SN-SCs alone (SN-SC group). Histological and functional analyses were performed 3 months later. The results indicated that in vitro, the expression levels of NGF, BDNF and GDNF were significantly higher, and in vivo, a more moderate amount of extracellular matrix was produced in the Fb&SC group than in the SN-SC group. Compared to the other groups, co-transplanting SN-Fbs with SCs at a 1:2 ratio had significantly positive effects on nerve regeneration and functional recovery.
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spelling pubmed-57239172017-12-11 The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair Wang, Yang Li, Dong Wang, Gangyang Chen, Lulu Chen, Jun Liu, Zhangyin Zhang, Zhaofeng Shen, Hua Jin, Yuqing Shen, Zunli Int J Biol Sci Research Paper Combinations of fibroblasts (Fbs) and corresponding epithelial cells have been widely used in many tissues, such as the skin and breast tissues, to augment tissue repair and remodeling. Recently, a large amount of new data has indicated that nerve Fbs play critical roles in Schwann cells (SCs) and axons in vitro. However, little is known regarding the effects of co-transplanting nerve Fbs and SCs on peripheral nerve repair in vivo. The aim of this study was to investigate the effect of co-transplanting sciatic nerve Fbs (SN-Fbs) and sciatic nerve SCs (SN-SCs) on nerve regeneration. We developed a 5 mm nerve-defect model in mice using a polyurethane (PUR) catheter and then injected one of four different mixtures of cells into the catheters to form the following four groups: pure Matrigel (Control group), SN-Fbs alone (SN-Fb group), SN-Fbs combined with SN-SCs at a ratio of 1:2 (Fb&SC group) and SN-SCs alone (SN-SC group). Histological and functional analyses were performed 3 months later. The results indicated that in vitro, the expression levels of NGF, BDNF and GDNF were significantly higher, and in vivo, a more moderate amount of extracellular matrix was produced in the Fb&SC group than in the SN-SC group. Compared to the other groups, co-transplanting SN-Fbs with SCs at a 1:2 ratio had significantly positive effects on nerve regeneration and functional recovery. Ivyspring International Publisher 2017-11-02 /pmc/articles/PMC5723917/ /pubmed/29230099 http://dx.doi.org/10.7150/ijbs.21976 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Wang, Yang
Li, Dong
Wang, Gangyang
Chen, Lulu
Chen, Jun
Liu, Zhangyin
Zhang, Zhaofeng
Shen, Hua
Jin, Yuqing
Shen, Zunli
The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair
title The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair
title_full The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair
title_fullStr The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair
title_full_unstemmed The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair
title_short The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair
title_sort effect of co-transplantation of nerve fibroblasts and schwann cells on peripheral nerve repair
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5723917/
https://www.ncbi.nlm.nih.gov/pubmed/29230099
http://dx.doi.org/10.7150/ijbs.21976
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