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Virulence evolution of a sterilizing plant virus: Tuning multiplication and resource exploitation
Virulence evolution may have far-reaching consequences for virus epidemiology and emergence, and virologists have devoted increasing effort to understand the modulators of this process. However, still little is known on the mechanisms and determinants of virulence evolution in sterilizing viruses th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724401/ https://www.ncbi.nlm.nih.gov/pubmed/29250431 http://dx.doi.org/10.1093/ve/vex033 |
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author | Vijayan, Viji López-González, Silvia Sánchez, Flora Ponz, Fernando Pagán, Israel |
author_facet | Vijayan, Viji López-González, Silvia Sánchez, Flora Ponz, Fernando Pagán, Israel |
author_sort | Vijayan, Viji |
collection | PubMed |
description | Virulence evolution may have far-reaching consequences for virus epidemiology and emergence, and virologists have devoted increasing effort to understand the modulators of this process. However, still little is known on the mechanisms and determinants of virulence evolution in sterilizing viruses that, as they prevent host reproduction, may have devastating effects on host populations. Theory predicts that sterilizing parasites, including viruses, would evolve towards lower virulence and absolute host sterilization to optimize the exploitation of host resources and maximize fitness. However, this hypothesis has seldom been analyzed experimentally. We investigated the evolution of virulence of the sterilizing plant virus Turnip mosaic virus (TuMV) in its natural host Arabidopsis thaliana by serial passage experiments. After passaging, we quantified virus accumulation and infectivity, the effect of infection on plant growth and development, and virulence of the ancestral and passaged viral genotypes in A. thaliana. Results indicated that serial passaging increased the proportion of infected plants showing absolute sterility, reduced TuMV virulence, and increased virus multiplication and infectivity. Genomic comparison of the ancestral and passaged TuMV genotypes identified significant mutation clustering in the P1, P3, and 6K2 proteins, suggesting a role of these viral proteins in the observed phenotypic changes. Our results support theoretical predictions on the evolution of virulence of sterilizing parasites and contribute to better understand the phenotypic and genetic changes associated with this process. |
format | Online Article Text |
id | pubmed-5724401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-57244012017-12-15 Virulence evolution of a sterilizing plant virus: Tuning multiplication and resource exploitation Vijayan, Viji López-González, Silvia Sánchez, Flora Ponz, Fernando Pagán, Israel Virus Evol Research Article Virulence evolution may have far-reaching consequences for virus epidemiology and emergence, and virologists have devoted increasing effort to understand the modulators of this process. However, still little is known on the mechanisms and determinants of virulence evolution in sterilizing viruses that, as they prevent host reproduction, may have devastating effects on host populations. Theory predicts that sterilizing parasites, including viruses, would evolve towards lower virulence and absolute host sterilization to optimize the exploitation of host resources and maximize fitness. However, this hypothesis has seldom been analyzed experimentally. We investigated the evolution of virulence of the sterilizing plant virus Turnip mosaic virus (TuMV) in its natural host Arabidopsis thaliana by serial passage experiments. After passaging, we quantified virus accumulation and infectivity, the effect of infection on plant growth and development, and virulence of the ancestral and passaged viral genotypes in A. thaliana. Results indicated that serial passaging increased the proportion of infected plants showing absolute sterility, reduced TuMV virulence, and increased virus multiplication and infectivity. Genomic comparison of the ancestral and passaged TuMV genotypes identified significant mutation clustering in the P1, P3, and 6K2 proteins, suggesting a role of these viral proteins in the observed phenotypic changes. Our results support theoretical predictions on the evolution of virulence of sterilizing parasites and contribute to better understand the phenotypic and genetic changes associated with this process. Oxford University Press 2017-11-06 /pmc/articles/PMC5724401/ /pubmed/29250431 http://dx.doi.org/10.1093/ve/vex033 Text en © The Author 2017. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Vijayan, Viji López-González, Silvia Sánchez, Flora Ponz, Fernando Pagán, Israel Virulence evolution of a sterilizing plant virus: Tuning multiplication and resource exploitation |
title | Virulence evolution of a sterilizing plant virus: Tuning multiplication and resource exploitation |
title_full | Virulence evolution of a sterilizing plant virus: Tuning multiplication and resource exploitation |
title_fullStr | Virulence evolution of a sterilizing plant virus: Tuning multiplication and resource exploitation |
title_full_unstemmed | Virulence evolution of a sterilizing plant virus: Tuning multiplication and resource exploitation |
title_short | Virulence evolution of a sterilizing plant virus: Tuning multiplication and resource exploitation |
title_sort | virulence evolution of a sterilizing plant virus: tuning multiplication and resource exploitation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724401/ https://www.ncbi.nlm.nih.gov/pubmed/29250431 http://dx.doi.org/10.1093/ve/vex033 |
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