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Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells
Human adult skeletal muscle has a limited ability to regenerate after injury and therapeutic options for volumetric muscle loss are few. Technologies to enhance regeneration of tissues generally rely upon bioscaffolds to mimic aspects of the tissue extracellular matrix (ECM). In the present study, s...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724504/ https://www.ncbi.nlm.nih.gov/pubmed/27878977 http://dx.doi.org/10.1002/term.2227 |
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author | Chaturvedi, Vishal Naskar, Deboki Kinnear, Beverley F. Grenik, Elizabeth Dye, Danielle E. Grounds, Miranda D. Kundu, Subhas C. Coombe, Deirdre R. |
author_facet | Chaturvedi, Vishal Naskar, Deboki Kinnear, Beverley F. Grenik, Elizabeth Dye, Danielle E. Grounds, Miranda D. Kundu, Subhas C. Coombe, Deirdre R. |
author_sort | Chaturvedi, Vishal |
collection | PubMed |
description | Human adult skeletal muscle has a limited ability to regenerate after injury and therapeutic options for volumetric muscle loss are few. Technologies to enhance regeneration of tissues generally rely upon bioscaffolds to mimic aspects of the tissue extracellular matrix (ECM). In the present study, silk fibroins from four Lepidoptera (silkworm) species engineered into three‐dimensional scaffolds were examined for their ability to support the differentiation of primary human skeletal muscle myoblasts. Human skeletal muscle myoblasts (HSMMs) adhered, spread and deposited extensive ECM on all the scaffolds, but immunofluorescence and quantitative polymerase chain reaction analysis of gene expression revealed that myotube formation occurred differently on the various scaffolds. Bombyx mori fibroin scaffolds supported formation of long, well‐aligned myotubes, whereas on Antheraea mylitta fibroin scaffolds the myotubes were thicker and shorter. Myotubes were oriented in two perpendicular layers on Antheraea assamensis scaffolds, and scaffolds of Philosamia/Samia ricini (S. ricini) fibroin poorly supported myotube formation. These differences were not caused by fibroin composition per se, as HSMMs adhered to, proliferated on and formed striated myotubes on all four fibroins presented as two‐dimensional fibroin films. The Young's modulus of A. mylitta and B. mori scaffolds mimicked that of normal skeletal muscle, but A. assamensis and S. ricini scaffolds were more flexible. The present study demonstrates that although myoblasts deposit matrix onto fibroin scaffolds and create a permissive environment for cell proliferation, a scaffold elasticity resembling that of normal muscle is required for optimal myotube length, alignment, and maturation. © 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. StartCopTextStartCopText© 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. |
format | Online Article Text |
id | pubmed-5724504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57245042017-12-12 Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells Chaturvedi, Vishal Naskar, Deboki Kinnear, Beverley F. Grenik, Elizabeth Dye, Danielle E. Grounds, Miranda D. Kundu, Subhas C. Coombe, Deirdre R. J Tissue Eng Regen Med Research Articles Human adult skeletal muscle has a limited ability to regenerate after injury and therapeutic options for volumetric muscle loss are few. Technologies to enhance regeneration of tissues generally rely upon bioscaffolds to mimic aspects of the tissue extracellular matrix (ECM). In the present study, silk fibroins from four Lepidoptera (silkworm) species engineered into three‐dimensional scaffolds were examined for their ability to support the differentiation of primary human skeletal muscle myoblasts. Human skeletal muscle myoblasts (HSMMs) adhered, spread and deposited extensive ECM on all the scaffolds, but immunofluorescence and quantitative polymerase chain reaction analysis of gene expression revealed that myotube formation occurred differently on the various scaffolds. Bombyx mori fibroin scaffolds supported formation of long, well‐aligned myotubes, whereas on Antheraea mylitta fibroin scaffolds the myotubes were thicker and shorter. Myotubes were oriented in two perpendicular layers on Antheraea assamensis scaffolds, and scaffolds of Philosamia/Samia ricini (S. ricini) fibroin poorly supported myotube formation. These differences were not caused by fibroin composition per se, as HSMMs adhered to, proliferated on and formed striated myotubes on all four fibroins presented as two‐dimensional fibroin films. The Young's modulus of A. mylitta and B. mori scaffolds mimicked that of normal skeletal muscle, but A. assamensis and S. ricini scaffolds were more flexible. The present study demonstrates that although myoblasts deposit matrix onto fibroin scaffolds and create a permissive environment for cell proliferation, a scaffold elasticity resembling that of normal muscle is required for optimal myotube length, alignment, and maturation. © 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. StartCopTextStartCopText© 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. John Wiley and Sons Inc. 2016-11-22 2017-11 /pmc/articles/PMC5724504/ /pubmed/27878977 http://dx.doi.org/10.1002/term.2227 Text en © 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Chaturvedi, Vishal Naskar, Deboki Kinnear, Beverley F. Grenik, Elizabeth Dye, Danielle E. Grounds, Miranda D. Kundu, Subhas C. Coombe, Deirdre R. Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells |
title | Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells |
title_full | Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells |
title_fullStr | Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells |
title_full_unstemmed | Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells |
title_short | Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells |
title_sort | silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724504/ https://www.ncbi.nlm.nih.gov/pubmed/27878977 http://dx.doi.org/10.1002/term.2227 |
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