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Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans
There is considerable insight into pathways and genes associated with heat-stress conditions. Most genes involved in stress response have been identified using mutant screens or gene knockdowns. Yet, there is limited understanding of the temporal dynamics of global gene expression in stressful envir...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724892/ https://www.ncbi.nlm.nih.gov/pubmed/29228038 http://dx.doi.org/10.1371/journal.pone.0189445 |
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author | Jovic, Katharina Sterken, Mark G. Grilli, Jacopo Bevers, Roel P. J. Rodriguez, Miriam Riksen, Joost A. G. Allesina, Stefano Kammenga, Jan E. Snoek, L. Basten |
author_facet | Jovic, Katharina Sterken, Mark G. Grilli, Jacopo Bevers, Roel P. J. Rodriguez, Miriam Riksen, Joost A. G. Allesina, Stefano Kammenga, Jan E. Snoek, L. Basten |
author_sort | Jovic, Katharina |
collection | PubMed |
description | There is considerable insight into pathways and genes associated with heat-stress conditions. Most genes involved in stress response have been identified using mutant screens or gene knockdowns. Yet, there is limited understanding of the temporal dynamics of global gene expression in stressful environments. Here, we studied global gene expression profiles during 12 hours of heat stress in the nematode C. elegans. Using a high-resolution time series of increasing stress exposures, we found a distinct shift in gene expression patterns between 3–4 hours into the stress response, separating an initially highly dynamic phase from a later relatively stagnant phase. This turning point in expression dynamics coincided with a phenotypic turning point, as shown by a strong decrease in movement, survival and, progeny count in the days following the stress. Both detectable at transcriptional and phenotypic level, this study pin-points a relatively small time frame during heat stress at which enough damage is accumulated, making it impossible to recover the next few days. |
format | Online Article Text |
id | pubmed-5724892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57248922017-12-15 Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans Jovic, Katharina Sterken, Mark G. Grilli, Jacopo Bevers, Roel P. J. Rodriguez, Miriam Riksen, Joost A. G. Allesina, Stefano Kammenga, Jan E. Snoek, L. Basten PLoS One Research Article There is considerable insight into pathways and genes associated with heat-stress conditions. Most genes involved in stress response have been identified using mutant screens or gene knockdowns. Yet, there is limited understanding of the temporal dynamics of global gene expression in stressful environments. Here, we studied global gene expression profiles during 12 hours of heat stress in the nematode C. elegans. Using a high-resolution time series of increasing stress exposures, we found a distinct shift in gene expression patterns between 3–4 hours into the stress response, separating an initially highly dynamic phase from a later relatively stagnant phase. This turning point in expression dynamics coincided with a phenotypic turning point, as shown by a strong decrease in movement, survival and, progeny count in the days following the stress. Both detectable at transcriptional and phenotypic level, this study pin-points a relatively small time frame during heat stress at which enough damage is accumulated, making it impossible to recover the next few days. Public Library of Science 2017-12-11 /pmc/articles/PMC5724892/ /pubmed/29228038 http://dx.doi.org/10.1371/journal.pone.0189445 Text en © 2017 Jovic et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Jovic, Katharina Sterken, Mark G. Grilli, Jacopo Bevers, Roel P. J. Rodriguez, Miriam Riksen, Joost A. G. Allesina, Stefano Kammenga, Jan E. Snoek, L. Basten Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans |
title | Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans |
title_full | Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans |
title_fullStr | Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans |
title_full_unstemmed | Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans |
title_short | Temporal dynamics of gene expression in heat-stressed Caenorhabditis elegans |
title_sort | temporal dynamics of gene expression in heat-stressed caenorhabditis elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724892/ https://www.ncbi.nlm.nih.gov/pubmed/29228038 http://dx.doi.org/10.1371/journal.pone.0189445 |
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