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Comprehensive investigation of cytokine- and immune-related gene variants in HBV-associated hepatocellular carcinoma patients
Host genotype may be closely related to the different outcomes of Hepatitis B virus (HBV) infection. To identify the association of variants and HBV infection, we comprehensively investigated the cytokine- and immune-related gene mutations in patients with HBV associated hepatocellular carcinoma (HB...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725607/ https://www.ncbi.nlm.nih.gov/pubmed/29138264 http://dx.doi.org/10.1042/BSR20171263 |
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author | Yu, Fengxue Zhang, Xiaolin Tian, Suzhai Geng, Lianxia Xu, Weili Ma, Ning Wang, Mingbang Jia, Yuan Liu, Xuechen Ma, Junji Quan, Yuan Zhang, Chaojun Guo, Lina An, Wenting Liu, Dianwu |
author_facet | Yu, Fengxue Zhang, Xiaolin Tian, Suzhai Geng, Lianxia Xu, Weili Ma, Ning Wang, Mingbang Jia, Yuan Liu, Xuechen Ma, Junji Quan, Yuan Zhang, Chaojun Guo, Lina An, Wenting Liu, Dianwu |
author_sort | Yu, Fengxue |
collection | PubMed |
description | Host genotype may be closely related to the different outcomes of Hepatitis B virus (HBV) infection. To identify the association of variants and HBV infection, we comprehensively investigated the cytokine- and immune-related gene mutations in patients with HBV associated hepatocellular carcinoma (HBV-HCC). Fifty-three HBV-HCC patients, 53 self-healing cases (SH) with HBV infection history and 53 healthy controls (HCs) were recruited, the whole exon region of 404 genes were sequenced at >900× depth. Comprehensive variants and gene levels were compared between HCC and HC, and HCC and SH. Thirty-nine variants (adjusted P<0.0001, Fisher’s exact test) and 11 genes (adjusted P<0.0001, optimal unified approach for rare variant association test (SKAT-O) gene level test) were strongly associated with HBV-HCC. Thirty-four variants were from eight human leukocyte antigen (HLA) genes that were previously reported to be associated with HBV-HCC. The novelties of our study are: five variants (rs579876, rs579877, rs368692979, NM_145007:c.*131_*130delTG, NM_139165:exon5:c.623-2->TT) from three genes (REAT1E, NOD-like receptor (NLR) protein 11 (NLRP11), hydroxy-carboxylic acid receptor 2 (HCAR2)) were found strongly associated with HBV-HCC. We found 39 different variants in 11 genes that were significantly related to HBV-HCC. Five of them were new findings. Our data implied that chronic hepatitis B patients who carry these variants are at a high risk of developing HCC. |
format | Online Article Text |
id | pubmed-5725607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57256072017-12-13 Comprehensive investigation of cytokine- and immune-related gene variants in HBV-associated hepatocellular carcinoma patients Yu, Fengxue Zhang, Xiaolin Tian, Suzhai Geng, Lianxia Xu, Weili Ma, Ning Wang, Mingbang Jia, Yuan Liu, Xuechen Ma, Junji Quan, Yuan Zhang, Chaojun Guo, Lina An, Wenting Liu, Dianwu Biosci Rep Research Articles Host genotype may be closely related to the different outcomes of Hepatitis B virus (HBV) infection. To identify the association of variants and HBV infection, we comprehensively investigated the cytokine- and immune-related gene mutations in patients with HBV associated hepatocellular carcinoma (HBV-HCC). Fifty-three HBV-HCC patients, 53 self-healing cases (SH) with HBV infection history and 53 healthy controls (HCs) were recruited, the whole exon region of 404 genes were sequenced at >900× depth. Comprehensive variants and gene levels were compared between HCC and HC, and HCC and SH. Thirty-nine variants (adjusted P<0.0001, Fisher’s exact test) and 11 genes (adjusted P<0.0001, optimal unified approach for rare variant association test (SKAT-O) gene level test) were strongly associated with HBV-HCC. Thirty-four variants were from eight human leukocyte antigen (HLA) genes that were previously reported to be associated with HBV-HCC. The novelties of our study are: five variants (rs579876, rs579877, rs368692979, NM_145007:c.*131_*130delTG, NM_139165:exon5:c.623-2->TT) from three genes (REAT1E, NOD-like receptor (NLR) protein 11 (NLRP11), hydroxy-carboxylic acid receptor 2 (HCAR2)) were found strongly associated with HBV-HCC. We found 39 different variants in 11 genes that were significantly related to HBV-HCC. Five of them were new findings. Our data implied that chronic hepatitis B patients who carry these variants are at a high risk of developing HCC. Portland Press Ltd. 2017-12-12 /pmc/articles/PMC5725607/ /pubmed/29138264 http://dx.doi.org/10.1042/BSR20171263 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Yu, Fengxue Zhang, Xiaolin Tian, Suzhai Geng, Lianxia Xu, Weili Ma, Ning Wang, Mingbang Jia, Yuan Liu, Xuechen Ma, Junji Quan, Yuan Zhang, Chaojun Guo, Lina An, Wenting Liu, Dianwu Comprehensive investigation of cytokine- and immune-related gene variants in HBV-associated hepatocellular carcinoma patients |
title | Comprehensive investigation of cytokine- and immune-related gene variants in HBV-associated hepatocellular carcinoma patients |
title_full | Comprehensive investigation of cytokine- and immune-related gene variants in HBV-associated hepatocellular carcinoma patients |
title_fullStr | Comprehensive investigation of cytokine- and immune-related gene variants in HBV-associated hepatocellular carcinoma patients |
title_full_unstemmed | Comprehensive investigation of cytokine- and immune-related gene variants in HBV-associated hepatocellular carcinoma patients |
title_short | Comprehensive investigation of cytokine- and immune-related gene variants in HBV-associated hepatocellular carcinoma patients |
title_sort | comprehensive investigation of cytokine- and immune-related gene variants in hbv-associated hepatocellular carcinoma patients |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5725607/ https://www.ncbi.nlm.nih.gov/pubmed/29138264 http://dx.doi.org/10.1042/BSR20171263 |
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